化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Trityl-Thiohexyl Amidite is used to functionalize the 5' end of an oligonucleotide with a thiol group. Thiol functionalization allows attachment to fluorescent dyes, maleimide compounds or conjugation to proteins through disulfide linkages. The lipophilic trityl group can be used as a handle for RP purification. It cannot be removed using normal acidic deblock methods.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 5' Phosphate Amidite enables the introduction of a phosphate group to the 5' terminus of an oligonucleotide. Oligonucleotides having a 5'-phosphate group are valuable tools for many molecular biology applications including gene construction, cloning, mutagensis, and ligation chain reaction. Quantification of the DMT protecting group removed during the standard synthesis deblocking step will yield phosphorylation efficiency.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 该化合物通常用于向寡核苷酸中添加氨基官能团。为了获得最佳结果，在氨脱保护期间将MMT留在寡核苷酸上。MMT基团可以通过溶液相方法使用2.5％TFA水溶液或通过柱纯化（参见产品用法）在后面进行切割。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. This compound is typically used to add amino functionality to oligonucleotides. For best results leave the MMT on the oligonucleotide during ammonia deprotection. Subsequent cleavage of the MMT group can be accomplished by solution phase methods using 2.5% aqueous TFA or by column cartridge purification (see product usage). https://www.biosearchtech.com/products/synthesis-reagents/amidites/5-mmtamino-c6-modifier-mmt6aminohexyl-amidite

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Trityl-Thiohexyl Amidite is used to functionalize the 5' end of an oligonucleotide with a thiol group. Thiol functionalization allows attachment to fluorescent dyes, maleimide compounds or conjugation to proteins through disulfide linkages. The lipophilic trityl group can be used as a handle for RP purification. It cannot be removed using normal acidic deblock methods.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 5'-DMT-dU amidite is used for the placement of deoxy uridine either internally or on the 5' end of oligonucleotides.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Oligonucleotides having a 5'-phosphate group are valuable tools for many molecular biology applications including gene construction, cloning, mutagensis, and ligation chain reaction. Several methods have been developed to allow for the 5' phosphorylation of synthetic oligonucleotides. The most common is through the use of a 5'Phosphate Amidite (2-O-4,4'-dimethoxytrityl-2'-oxydiethane sulfonyl)-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite (BNS-5010). Unfortunately, the DMT group cannot be left on for DMT-ON purification due to the elimination reaction of the DMT and sulfonyl ethyl group during normal ammonium hydroxide deprotection and cleavage. Phosphorylating Amidite II contains a DMT group that can be removed to determine the coupling efficiency of the amidite or it may be left on to allow for reverse phase cartridge purification. To determine the coupling efficiency, the DMT group is removed on the synthesizer. The side chain is eliminated, during ammonium hydroxide deprotection and cleavage, to yield a 5'Phosphate. For DMT-ON purification, the DMT group is removed with aqueous acid (3% TFA) and the side chain is eliminated after treatment with ammonium hydroxide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 5' Phosphate Amidite enables the introduction of a phosphate group to the 5' terminus of an oligonucleotide. Oligonucleotides having a 5'-phosphate group are valuable tools for many molecular biology applications including gene construction, cloning, mutagensis, and ligation chain reaction. Quantification of the DMT protecting group removed during the standard synthesis deblocking step will yield phosphorylation efficiency.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 次黄嘌呤是一种通用碱基，因此可被掺入到合成寡核苷酸的任意位置。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide. Inosine is used as a universal base, therefore it can be incorporated into any position in a synthetic oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 该化合物通常用于向寡核苷酸中添加氨基官能团。为了获得最佳结果，在氨脱保护期间将MMT留在寡核苷酸上。MMT基团可以通过溶液相方法使用2.5％TFA水溶液或通过柱纯化（参见产品用法）在后面进行切割。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. This compound is typically used to add amino functionality to oligonucleotides. For best results leave the MMT on the oligonucleotide during ammonia deprotection. Subsequent cleavage of the MMT group can be accomplished by solution phase methods using 2.5% aqueous TFA or by column cartridge purification (see product usage). https://www.biosearchtech.com/products/synthesis-reagents/amidites/5-mmtamino-c6-modifier-mmt6aminohexyl-amidite

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 生物素用于标记合成寡核苷酸探针的5'端，可以通过酶标记的抗生物素蛋白或链霉亲和素偶联物进行检测。5'生物素亚酰胺只能添加到寡核苷酸的5'末端来终止合成。该亚酰胺具有DMT保护基团，DMT可以留在寡核苷酸上以备后续RP柱纯化。 Biotin is used in the 5' labeling of synthetic oligonucleotide probes. Biotin labeling can be detected through the use of enzyme labeled avidin or streptavidin conjugates. 5' Biotin amidite can only be added to the 5' terminus of the oligonucleotide, thus terminating the synthesis. This amidite has a DMT group which may be left on for RP cartridge purification. https://www.biosearchtech.com/products/synthesis-reagents/amidites/5-biotin-amidite-ndmtbiotinyl2aminoethoxyethanol

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 DABCYL is a quencher which absorbs between 400 to 525 nm and has maximum quenching at 474 nm. DABCYL Amidite is used for 5' labeling of oligonucleotides and can be used to synthesize dual labeled probes and molecular beacons™ by starting with a 3' fluorescent CPG.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 5'-DMT-dU amidite is used for the placement of deoxy uridine either internally or on the 5' end of oligonucleotides.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 次黄嘌呤是一种通用碱基，因此可被掺入到合成寡核苷酸的任意位置。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide. Inosine is used as a universal base, therefore it can be incorporated into any position in a synthetic oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Chemically synthesized DNA in the form of probes, primers and other oligonucleotides are used in a variety of applications such as PCR, genome sequencing, SNP genotyping, and gene synthesis. To enable the synthesis of sophisticated probes for q-PCR, LGC Biosearch offers a broad range of, fluorophore reporter and non-fluorescing quencher phosphoramidites. Our proprietary Black Hole Quencher®(BHQ®)dye phosphoramidites are designed to permit efficient quenching of all fluorophores making it possible to utilize reporter dyes that emit anywhere across the visible spectrum from 480 nm into the near IR. Our CAL Fluor® and Quasar® dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. LGC Biosearch also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. These amidites can be used for direct incorporation into the modified oligonucleotide.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 Thiol Modifier C6-S-S Amidite is used to functionalize the 5' terminus of an oligonucleotide with a thiol group. Thiol modifications have become significant in the production of diagnostic probes. Thiol functionalization allows attachment to fluorescent dyes, maleimide compounds or conjugation to proteins through disulfide linkages. The DMT group can be used as a handle for RP purification and later removed with 100 mM DTT.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 CAL Fluor® Gold 540是一种在可见光谱的黄绿色区域具有发射荧光的染料。CAL Fluor® Gold 540亚磷酰胺可用在5'核酸酶检测、分子信标、Scorpions®引物以及其它检测实验中用来标记荧光探针的5'端。该亚酰胺不含有DMT保护基团，并且只能加到寡核苷酸的5'末端。CAL Fluor® Gold 540荧光基团可用来替代TET，且可被BHQ-1黑洞淬灭剂淬灭。 CAL Fluor® Gold 540 dye fluoresces in the yellow green region of the visible spectrum. CAL Fluor Gold 540 amidite is used for the 5' labeling of fluorogenic probes used in 5' nuclease assays, Molecular Beacons, Scorpions® primers and other detection assays. This amidite does not contain a DMT protecting group and can only be added to the 5' terminus of the oligo. CAL Fluor Gold 540 fluorophore is an alternative for TET and is quenched by BHQ®-1.

化学合成的DNA如探针、引物和其它寡核苷酸具有广泛的应用，可用于PCR，基因组测序，SNP基因分型和基因合成等。 LGC Biosearch提供多种亚磷酰胺修饰的荧光报告染料和非荧光淬灭染料，可用于合成复杂的qPCR探针。 其专利的Black Hole Quencher®（BHQ®）染料亚磷酰胺能够有效淬灭所有荧光基团，因此从480nm到近红外可见光谱范围内发射荧光的任何报告染料都可以与其搭配使用。 CAL Fluor®和Quasar®荧光基团亚磷酰胺的发射光谱跨越可见光和近红外光谱，是多重实时qPCR的理想选择。 此外，LGC Biosearch还提供多种修饰如生物素、氨基、巯基、间臂以及其它常用荧光染料的亚磷酰胺。这些亚酰胺可用于直接掺入修饰的寡核苷酸中。 CAL Fluor® Orange 560是一种在可见光谱的黄橙色区域具有发射荧光的染料。CAL Fluor® Orange 560亚磷酰胺可用在5'核酸酶检测、分子信标、Scorpions®引物以及其它检测实验中用来标记荧光探针的5'端。该亚酰胺不含有DMT保护基团，并且只能加到寡核苷酸的5'末端。CAL Fluor® Orange 560荧光基团可用来替代VIC和HEX染料，且可被BHQ-1黑洞淬灭剂淬灭。 CAL Fluor® Orange 560 is an amidite which fluoresces in the yellow-orange region of the visible spectrum. CAL Fluor Orange 560 amidite is used for the 5' labeling of fluorogenic probes used in 5' nuclease assays, Molecular Beacons™, and other genomic assays. This amidite does not contain a DMT protecting group and therefore can only be added to the 5' terminus of the oligo. BHQ®-1 will quench the CAL Fluor Orange 560 moiety. CAL Fluor Orange 560 is an alternative for VIC™ and HEX™.