LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
LCG Biosearch Micropure purification columns are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a column or syringe barrel packed with high performance hydrophobic polymeric resin. The resin allows for strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product. The desired product (free from organic residues) is eluted with 20% acetonitrile. Using the DNA purification columns in conjunction with LGC Biosearch’s Microsync ll vacuum manifold system allows for rapid, efficient purification of many samples simultaneously. Micropure columns may also be used for the simple desalting of oligonucleotides.
Oligonucleotides (whether synthetic or natural) may be purified by a number of techniques, including polyacrylamide gel electrophoresis and high performance liquid chromatography (either by ion exchange or reverse-phase methods). Synthetic DNA can be conveniently de-salted and purified by reverse-phase low-pressure separation on BTI's SuperPure (SP-1000) columns. BTI's SuperPure Columns are intended for reverse-phase purification of 5'-DMT oligonucleotides followed by on-column detritylation. The method relies on strong binding between the 5'-DMT protected product and a hydrophobic optimized polymer packing (polystyrene), thus allowing separation from truncated sequences which, due to a lack of DMT group, do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and permits many samples to be purified simultaneously. The SuperPure column has capacity to handle crude, cleaved DNA from a 50 nmol scale synthesis. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
LCG Biosearch Micropure purification columns are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a column or syringe barrel packed with high performance hydrophobic polymeric resin. The resin allows for strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product. The desired product (free from organic residues) is eluted with 20% acetonitrile. Using the DNA purification columns in conjunction with LGC Biosearch’s Microsync ll vacuum manifold system allows for rapid, efficient purification of many samples simultaneously. Micropure columns may also be used for the simple desalting of oligonucleotides.
Oligonucleotides (whether synthetic or natural) may be purified by a number of techniques, including polyacrylamide gel electrophoresis and high performance liquid chromatography (either by ion exchange or reverse-phase methods). Synthetic DNA can be conveniently de-salted and purified by reverse-phase low-pressure separation on BTI's SuperPure Plus (SP-2000) columns . BTI's SuperPure Columns are intended for reverse-phase purification of 5'-DMT oligonucleotides followed by on-column detritylation. The method relies on strong binding between the 5'-DMT protected product and a hydrophobic optimized polymer packing (polystyrene), thus allowing separation from truncated sequences which, due to a lack of DMT group, do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and permits many samples to be purified simultaneously. Crude DNA from a 200 nmol synthesis scale can be loaded on the SuperPure Plus. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
BTI's MicroPure II columns (MP-1602) are intended for Reversed-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a 5 mL syringe barrel packed with 200 mg of high performance hydrophobic polymeric resin. The method relies on strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and many samples can be purified simultaneously. At least 1 µmol or 50 O.D's of crude DNA can be applied to the column. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
这些1/16和1/8英寸的聚乙烯玻璃料是一种多孔材料,用于维持柱内的合成支撑面。它们应与我们的标准DNA合成柱(CL-1501或CL-1502)一起使用,来制作您自己的特殊柱子。玻璃料孔径为15-45μm,与所有DNA合成试剂兼容。
These 1/16 and 1/8 inch polyethylene frits are a porous material designed to retain the synthesis support within the column. They should be used with our standard DNA synthesis columns (CL-1501 or CL-1502) to make your own specialty columns. The frit pore size is 15-45µm. They are compatible with all DNA synthesis reagents.
这些1/16和1/8英寸的聚乙烯玻璃料是一种多孔材料,用于维持柱内的合成支撑面。它们应与我们的标准DNA合成柱(CL-1501或CL-1502)一起使用,来制作您自己的特殊柱子。玻璃料孔径为15-45μm,与所有DNA合成试剂兼容。
These 1/16 and 1/8 inch polyethylene frits are a porous material designed to retain the synthesis support within the column. They should be used with our standard DNA synthesis columns (CL-1501 or CL-1502) to make your own specialty columns. The frit pore size is 15-45µm. They are compatible with all DNA synthesis reagents.
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
LCG Biosearch Micropure purification columns are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a column or syringe barrel packed with high performance hydrophobic polymeric resin. The resin allows for strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product. The desired product (free from organic residues) is eluted with 20% acetonitrile. Using the DNA purification columns in conjunction with LGC Biosearch’s Microsync ll vacuum manifold system allows for rapid, efficient purification of many samples simultaneously. Micropure columns may also be used for the simple desalting of oligonucleotides.
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
LCG Biosearch Micropure purification columns are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a column or syringe barrel packed with high performance hydrophobic polymeric resin. The resin allows for strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product. The desired product (free from organic residues) is eluted with 20% acetonitrile. Using the DNA purification columns in conjunction with LGC Biosearch’s Microsync ll vacuum manifold system allows for rapid, efficient purification of many samples simultaneously. Micropure columns may also be used for the simple desalting of oligonucleotides.
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
BTI's MicroPure II columns (MP-1602) are intended for Reversed-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a 5 mL syringe barrel packed with 200 mg of high performance hydrophobic polymeric resin. The method relies on strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and many samples can be purified simultaneously. At least 1 µmol or 50 O.D's of crude DNA can be applied to the column. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
Oligonucleotides (whether synthetic or natural) may be purified by a number of techniques, including polyacrylamide gel electrophoresis and high performance liquid chromatography (either by ion exchange or reverse-phase methods). Synthetic DNA can be conveniently de-salted and purified by reverse-phase low-pressure separation on BTI's SuperPure (SP-1000) columns. BTI's SuperPure Columns are intended for reverse-phase purification of 5'-DMT oligonucleotides followed by on-column detritylation. The method relies on strong binding between the 5'-DMT protected product and a hydrophobic optimized polymer packing (polystyrene), thus allowing separation from truncated sequences which, due to a lack of DMT group, do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and permits many samples to be purified simultaneously. The SuperPure column has capacity to handle crude, cleaved DNA from a 50 nmol scale synthesis. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
Oligonucleotides (whether synthetic or natural) may be purified by a number of techniques, including polyacrylamide gel electrophoresis and high performance liquid chromatography (either by ion exchange or reverse-phase methods). Synthetic DNA can be conveniently de-salted and purified by reverse-phase low-pressure separation on BTI's SuperPure Plus (SP-2000) columns . BTI's SuperPure Columns are intended for reverse-phase purification of 5'-DMT oligonucleotides followed by on-column detritylation. The method relies on strong binding between the 5'-DMT protected product and a hydrophobic optimized polymer packing (polystyrene), thus allowing separation from truncated sequences which, due to a lack of DMT group, do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and permits many samples to be purified simultaneously. Crude DNA from a 200 nmol synthesis scale can be loaded on the SuperPure Plus. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
LCG Biosearch Micropure purification columns are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a column or syringe barrel packed with high performance hydrophobic polymeric resin. The resin allows for strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product. The desired product (free from organic residues) is eluted with 20% acetonitrile. Using the DNA purification columns in conjunction with LGC Biosearch’s Microsync ll vacuum manifold system allows for rapid, efficient purification of many samples simultaneously. Micropure columns may also be used for the simple desalting of oligonucleotides.
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
LCG Biosearch Micropure purification columns are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a column or syringe barrel packed with high performance hydrophobic polymeric resin. The resin allows for strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product. The desired product (free from organic residues) is eluted with 20% acetonitrile. Using the DNA purification columns in conjunction with LGC Biosearch’s Microsync ll vacuum manifold system allows for rapid, efficient purification of many samples simultaneously. Micropure columns may also be used for the simple desalting of oligonucleotides.
LCG Biosearch Micropure纯化柱通过进行柱上去三苯甲基化,用于反向纯化5'-DMT(5'-二甲氧基三苯甲基)保护的寡核苷酸。净化滤芯由填充有高性能疏水聚合树脂的柱子或注射筒组成。树脂能够强力结合5'-DMT保护的产物,从而将不结合的常见杂质和截短序列分离。随后用酸处理即可从产物中除去DMT基团。用20%乙腈洗脱所需产物(无有机残余物)。将DNA纯化柱与LGC Biosearch的Microsync ll真空歧管系统结合使用,可同时快速,高效地纯化大量样品。微纯化柱也可以用于寡核苷酸的简单脱盐。
LCG Biosearch Micropure purification columns are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a column or syringe barrel packed with high performance hydrophobic polymeric resin. The resin allows for strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product. The desired product (free from organic residues) is eluted with 20% acetonitrile. Using the DNA purification columns in conjunction with LGC Biosearch’s Microsync ll vacuum manifold system allows for rapid, efficient purification of many samples simultaneously. Micropure columns may also be used for the simple desalting of oligonucleotides.