Monarch Gel Dissolving Buffer is designed for use with the Monarch DNA Gel Extraction Kit (T1020S/L). This is the buffer used to dissolve the agarose containing the target DNA. The buffer also conditions the DNA for subsequent binding to the column.
DNA纯化和胶回收试剂盒，超高性价比，优秀的实验表现，独特的环保设计 The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging
PCR产物纯化和胶回收试剂盒，超高性价比，优秀的实验表现，独特的环保设计 The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
EpiMark 甲基化 DNA 富集试剂盒可有效的富集基于 CpG 密度的双链 CpG 甲基化 DNA。利用人MBD2a蛋白的甲基-CpG结合域作为捕获诱饵，形成 MBD2a-Fc/Protein A磁珠复合物，可以选择性地结合CpG甲基化的双链DNA，高灵敏度和精确性。2小时内就能完成4步富集反应。提供MBD2-Fc蛋白，Protein A 磁珠，结合/洗涤缓冲液，高盐洗脱缓冲液，片段化 HeLa DNA，Line element 引物（作为甲基化基因座对照），RPL30 引物（作为非甲基化基因座对照），MirA 基因座对照引物
DNA纯化和胶回收试剂盒，超高性价比，优秀的实验表现，独特的环保设计 The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging
PCR产物纯化和胶回收试剂盒，超高性价比，优秀的实验表现，独特的环保设计 The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
NEBNext 微生物 DNA 富集试剂盒；该试剂盒适用于从甲基化宿主 DNA 的样品中（包括人源样品）富集微生物 DNA，主要是通过选择性结合并移除带有 CpG 甲基化的宿主 DNA 来达到富集微生物 DNA 的目的 The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment.
从组织和细胞中快速提取高浓度基因组DNA。5ul洗脱体积适用于微量样品。 Purification of genomic, bacterial, and viral DNA from smallest samples. • High sensitivity for DNA isolation from small sample quantities, like biopsy materials, forensic samples, or FACS sorted cells • Elution in as little as 5 μL resulting in highly concentrated DNA • Excellent DNA recovery and purity for reliable results • DNA ready to use for multiplex PCR and all common downstream applications
Applications* • DNA isolation from tissue (e.g., mouse kidney, laser micro-dissections) • DNA isolation from cells (e.g., bacteria, yeast, cultured cells) • DNA isolation from clinical samples (e.g., biopsy samples, fine needle aspirates) • DNA isolation from forensic samples (e.g., dried blood spots, buccal swabs, finger prints) • DNA suitable for sensitive applications (e.g., multiplex PCR, real-time PCR) NucleoSpin® Tissue XS Columns, Collection Tubes (2 mL), buffers, Proteinase K 10 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K
微量的DNA大片段快速回收浓缩，洗脱体积仅为6–10 µL Post clean-up and concentration of small amounts of large DNA fragments • Efficient post clean-up of large DNA fragments for successful downstream applications • Recommended for small DNA quantities extracted from, e.g., forensic samples • Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA • Easier and faster than microdialysis filtration units • 20 min/6 preps – time-saving procedure
快速从食物或饲料中小规模提取基因组DNA。能从复杂成分中分离微量降解的DNA，适用范围广泛，完全去除PCR抑制剂，得到高质量的DNA。 For rapid small-scale isolation of genomic DNA from food and feed • Complete removal of PCR inhibitors → get high quality DNA • Even low amounts of partially degraded DNA can be purified from complex matrices • Fast and easy procedure • DNA from various sample materials → highest flexibility
Technology: Silica-membrane technology Format: Mini spin columns Sample material: 5–200 mg Fragment size: 300 bp–approx. 50 kbp Typical yield: 0.1–10 µg A260/A280: 1.6–1.9 Elution volume: 100 µL Preparation time: 30 min/6 preps Binding capacity: 30 µg Applications • DNA from complex matrices, e.g., processed food, soy (milk and flour), chocolate, cereals, meat, honey or pollen, animal feed • Typical downstream applications: real-time PCR, Southern blotting, enzymatic reactions NucleoSpin® Food Columns, Collection Tubes (2 mL), buffers, Proteinase K 10 preps for the isolation of genomic DNA from food - NucleoSpin Food Columns, Collection Tubes, buffers, Proteinase K
For rapid purification of high-quality DNA from blood • Ready-to-use DNA in less than 30 min • Complete removal of PCR inhibitors • Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA • Consistently high DNA yields • Also suitable for isolation of viral DNA, or bacterial DNA from blood samples • Also available for QIAcube®
• Highest sensitivity for viral DNA • Proven for a large variety of viruses including HBV, CMV, HPV, TTV, EBV
Applications* • Genomic, bacterial, viral DNA isolation • DNA from whole blood (human or animal blood, fresh or frozen) • DNA from whole blood treated with citrate, EDTA, heparin, CPDA • DNA from serum, plasma, buffy coat, platelets, body fluids (e.g. amniotic fluid) • DNA from cultured cells • Viral DNA from blood, serum, plasma, and biological fluids • Genomic and bacterial DNA from blood and biological fluids • HBV, CMV, HPV, TTV, EBV • DNA suitable for (PCR, Southern blotting, enzymatic reactions, etc.) NucleoSpin® Blood Columns with Collection Tubes, Collection Tubes (2 mL), buffers, Proteinase K 10 preps for the isolation of genomic DNA from blood - NucleoSpin Blood Columns, Collection Tubes, buffers, Proteinase K
All-round kit with highest sensitivity for purification of total DNA from clinical or forensic samples, tissues, cells, yeast, bacteria, or viruses • High DNA recovery and purity • Reliable DNA purification for reproducible results • Suitable for a huge variety of starting materials –more than 16 optimized support protocols available
Applications* • DNA from tissue (e.g., mouse tails) • DNA from cells (e.g., cultured human or animal cells, bacteria, yeast) • DNA from clinical samples (e.g., stool, urine, biopsy samples) • DNA from forensic samples (e.g., dried blood spots, hair, buccal swabs, cigarette stubs) • DNA from FFPE tissue • DNA from blood sample storage cards (e.g., NucleoCard®, page 145) • Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions NucleoSpin® Tissue Columns, Collection Tubes(2 mL), buffers, Proteinase K 10 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K
从福尔马林固定石蜡包埋样品中轻松快速地提取高质量DNA。脱石蜡技术，无需二甲苯，洗脱体积小，产物浓度高 Improved DNA quality from formalin-fixed, paraffin-embedded samples – no xylene needed • Very easy paraffin removal – Paraffin Dissolver (patent pending) included • Concentrated DNA by elution in small volume • Overcoming formalin crosslinking of DNA by incubation in decrosslinking buffer • High quality DNA for improved performance in PCR
Technology: Silica-membrane technology Format: Mini spin columns – XS design Sample material: ≤ 7 sections (10 µm) of 250 mm2 total area (< 15 mg paraffin*) Typical yield: Depending on amount and quality of the sample DNA quality: Strongly depending on sample quality Elution volume: 5–30 µL Preparation time: 70 min/6 preps (excl. lysis) Binding capacity: 110 µg
Applications • Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples • Isolation of DNA from fresh and archived FFPE samples • Isolation of DNA from specimen of object slides • Typical downstream application: PCR NucleoSpin® FFPE DNA Columns, Collection Tubes (2 mL), Paraffin Dissolver, buffers, Proteinase K 10 preps for the purification of DNA from FFPE samples - NucleoSpin FFPE DNA Columns, Collection Tubes, Paraffin Dissolver, buffers, Proteinase K
高分子量DNA的后继纯化浓缩。快速高效回收100 bp–50 kb DNA.不含裂解液。 Post clean-up and concentration of large sized DNA • Effective post clean-up and concentration of large-sized DNA for successful downstream applications • High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp • Concentrate your DNA sample easily and faster compared to microdialysis filtrations units • 15 min/10 preps – time-saving procedure
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 150 µL aqueous DNA solution (< 25 µg DNA) Fragment size: 100 bp–approx. 50 kbp Typical recovery: 80–90 % A260/A280: 1.8–1.9 Elution volume: 50–100 µL Preparation time: 15 min/10 preps Binding capacity: 50 µg Applications • Clean-up and concentration of pre-purified DNA (organic extractions) • Clean-up and concentration of DNA from enzymatic reactions • Not recommended for gel or PCR clean-up • Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification NucleoSpin® gDNA Clean-up Columns,Collection Tubes (2 mL), buffers 10 preps for clean-up of genomic DNA in aqueous solutions - NucleoSpin gDNA Clean-up Columns, Collection Tubes, buffers
Pre-filled single spin columns for dye-terminator removal • Efficient removal of dye terminators (e.g., BigDye™ terminators) without ethanol precipitation • Convenient spin column format • Long-term storage at room temperature
Technology: Gel-filtration technology Format: Mini spin columns Sample: material 20 µL sequencing reaction mix Preparation time: 5 min/prep (wihout hydration of matrix) Applications Dye-terminator removal pre-filled NucleoSEQ® Columns, Collection Tubes (2 mL) pre-filled NucleoSEQ Columns, Collection Tubes pack of 10
For ultra-fast purification of highly concentrated Genomic DNA from blood and biological fluids • Ultra-fast procedure • Washing and drying in one combined step (hands-on-time < 10 min) • Consistently high DNA yields • Elution volume only 30–50 μL • Highly concentrated DNA, ready to use for sensitive downstream applications
Technology： Silica-membrane technology Format： Mini spin columns Sample material： 5–200 µL whole blood (human or animal, fresh or frozen), body fluids；< 5 x 10(6) cultured cells (lymphocytes) Fragment size: 200 bp–approx. 50 kbp Typical yield: 4–6 µg A260/A280: 1.6–1.9 Typical concentration: 80–120 ng/µL Elution volume: 30–50 µL Preparation time: ~ 25 min/prep Hands-on-time < 10 min Binding capacity: 50 µg
Applications* • DNA from whole blood (human or animal, fresh or frozen) • DNA from whole blood treated with citrate, EDTA, heparin, CPDA • DNA from buffy coat, platelets, body fluids (e.g., amniotic fluid), serum, plasma • DNA from cultured cells • Typical downstream applications: PCR, Southern blotting, enzymatic reactions NucleoSpin® Blood QuickPure Columns with Collection Tubes, Collection Tubes (2 mL), buffers, Proteinase K 10 preps for the isolation of genomic DNA from blood - NucleoSpin Blood QuickPure Columns, Collection Tubes, buffers, Proteinase K
