细胞或者组织RNA稳定剂，保持核酸的完整性。≤ 24 hours at 37 °C ≤ 7 days at 18–25 °C ≤ 1 month at 4 °C Long term at -20 °C or lower

快速高效地从血浆/血清样本中提取外泌体。 Exosome Precipitation Solutions – fast and efficient enrichment of exosomes from blood serum and plasma samples • Most efficient enrichment of exosomes for any downstream application; • Simple and fast precipitation of exosomes – avoid tedious ultra centrifugation; • Flexibility in sample amount – procedure can be scaled up or down depending on sample volume.

快速高效地从尿液样本中提取外泌体。 Exosome Precipitation Solutions – fast and efficient enrichment of exosomes from urine samples • Most efficient enrichment of exosomes for any downstream application; • Simple and fast precipitation of exosomes – avoid tedious ultra centrifugation; • Flexibility in sample amount – procedure can be scaled up or down depending on sample volume.

配套NucleoZOL试剂使用的RNA纯化柱，含buffer。 Combine NucleoZOL with proven NucleoSpin® technology;   • Total RNA (incl. miRNA) with a simple bind-wash-elute procedure; • Superior RNA yields due to the efficient NucleoZOL* lysis; • Save time and benefit from the easy and proven handling.

简单快速纯化或浓缩各种RNA样品。不含裂解试剂。柱载量200ug Simple, fast, and convenient clean-up of RNA; • Complete removal of RT-PCR inhibitors; • Time-saving procedure based on NucleoSpin® RNA II, without DNase digestions and homogenization steps; • RNA clean-up from pre-purified RNA (phenol/chloroform), enzymatic reactions (e.g. amplification reactions, labeling reactions.

快速高效浓缩纯化各种RNA样品。柱载量110ug，洗脱体积5-30ul. Highly efficient clean-up and concentration of RNA samples; • Complete removal of RT-PCR inhibitors; • Very high RNA recovery and concentration; • RNA clean-up from pre-purified RNA (phenol / chloroform), enzymatic reactions (e.g., amplification reactions, labeling reactions, DNase digestions). • Time-saving procedure based on NucleoSpin® RNA XS technology. • Input volume up to 300 μL • Elution in as little as 5 μL possible

The Monarch RNA Cleanup Wash Buffer, a component of the Monarch RNA Cleanup Kits (NEB #T2030, T2040, T2050), is used to wash RNA after binding to ensure the removal of contaminants such as enzyme, NTPs, and short RNA fragments. It is supplied as a 5X concentrate and requires dilution with ethanol (≥95%) before use. The product is supplied in 2 bottles, each with 20 ml of concentrated buffer.

从组织和细胞中快速提取6-700ug的更完整的RNA。离心柱式，载量达700ug。提供NucleoSpin® Filters Midi (shredders)有效降低裂解液粘稠度以均质化。提供rDNase实现纯化柱上的DNA降解，有效去除DNA污染。 Midi spin kit for the isolation of RNA of highest integrity; • Efficient removal of genomic DNA – rDNase included for on-column digestion; • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters Midi (shredders) included; • Up to 600 μg ready-to-use RNA.

采用单相提取技术，在使用时无需加入氯仿。NucleoZOL能够从各方面改善试剂法抽提RNA的操作体验，同时得到更好的抽提效果。 1.无需氯仿，无需分相，操作简单； 2.更少DNA/protein污染，更少的苯酚残留，RNA质量高； 3.适合多种样本(动植物细胞、组织、体液；酵母；细菌），RNA得率高；室温离心； 4.可以同时分离miRNA和长片段RNA 。

快速简洁地从1.3ml全血中提取高质量的RNA。无需预处理红细胞，产量高，无DNA污染，兼容常规采血管抗凝剂。 Midi spin kit for the isolation of RNA from fresh or frozen blood (human or animal); • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at room temperature); • Superior RNA yield and quality from up to 1.3 mL whole blood; • Efficient on-column DNA removal for an increased sensitivity in downstream applications; • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin).

快速从血浆中提取游离DNA，独特主体设计，可将洗脱体积减少到5ul. 更大上样量 720ul. For rapid purification of circulating DNA from plasma and serum; • High recovery even of fragmented DNA > 50 bp; • Elution in as little as 5 μL; • Concentrated DNA even from dilute samples; • DNA ready to use for real-time PCR.

简单快速提取植物样本中RNA，柱上消化gDNA。 Plant RNA mini spin kit – for all kinds of plant material. • Efficient removal of contaminating DNA – rDNase included for on-column digestion; • Efficient sample homogenization and reduction of viscosity –NucleoSpin® Filters (shredders) included; • Up to 70 μg ready-to-use RNA; • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set.

使用DNA结合柱去除基因组DNA，相对于使用DNA酶去除基因组DNA的试剂盒，DNA去除效果更好，速度更快。
New generation for efficient and quick RNA isolation from cells and tissues; • NucleoSpin® gDNA Removal Column – no time consuming rDNase digestion; • Convenient handling – lysate clearing and gDNA removal with one column in one step; • New efficient lysis buffer – no b-mercapthoethanol or TCEP necessary.

福尔马林固定石蜡包被的样本中轻松提取RNA。一盒两用，可提取miRNA及mRNA。试剂轻松去除石蜡，无需二甲苯。离心柱式提取，柱上去除DNA污染，操作简便。
Two-in-one kit for the isolation of small (e.g., miRNA) and large RNA from even very limited formalin-fixed, paraffin-embedded samples. • Blue colored Paraffin Dissolver (patent pending) included - very easy and convenient paraffin removal. • rDNase included – efficient on-column DNA removal. • High quality RNA for enhanced RT-PCR performance.

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (50 µg) rapidly and reliably purifies up to 50 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (10 µg) rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

使用DNA结合柱去除基因组DNA，相对于使用DNA酶去除基因组DNA的试剂盒，DNA去除效果更好，速度更快。XS Column 更适合微量样本提取。
New generation for efficient and fast RNA isolation from smallest samples； • Developed for extra small samples down to single cell analysis； • gDNA removal column to omit time consuming DNA digestion； • Novel lysis buffer without mercapthoethanol or TCEP。

快速简便地从<400ul全血中提取高质量RNA。产量高，无需预处理红细胞，无DNA污染，兼容常规采血管和抗凝剂。 Isolation of RNA from fresh or frozen whole blood (human or animal) ; • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4°C – complete processing at room temperature); • Superior RNA yield and quality from up to 400 µL whole blood;; • Efficient on-column DNA removal for an increased sensitivity in downstream applications; • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin).

NucleoSpin RNA Plant 升级版，内有裂解管，更充分的裂解。特殊缓冲液可以更好的去除次生代谢产物，提高提取效率。
Universal kit for challenging plant and fungal samples: • Tailored protocols for diverse starting materials; • Convenient handling and efficient isolation of high integrity RNA; • NucleoSpin® RNA Plant and Fungi filter columns for lysate clearing included;

从福尔马林固定石蜡包被的样本中轻松提取RNA。试剂轻松去除石蜡，无需二甲苯。离心柱式提取，柱上去除DNA污染，操作简便，洗脱体积小，RNA浓度高，更完整。 Improved RNA quality from formalin-fixed, paraffin-embedded samples Paraffin Dissolver: no use of xylene required. • Enhanced RT-PCR performance – RNA is well decrosslinked and less fragmented; • Very easy paraffin removal – Paraffin Dissolver (patent pending) included; • Minimum number of working steps in a streamlined workflow; • Efficient on-column DNA removal, rDNase included; • Higher sensitivity with concentrated RNA – elution volume as little as 5 μL.

福尔马林固定石蜡包被的样本中轻松提取RNA。一盒两用，可提取miRNA及mRNA。试剂轻松去除石蜡，无需二甲苯。离心柱式提取，柱上去除DNA污染，操作简便。
Two-in-one kit for the isolation of small (e.g., miRNA) and large RNA from even very limited formalin-fixed, paraffin-embedded samples. • Blue colored Paraffin Dissolver (patent pending) included - very easy and convenient paraffin removal. • rDNase included – efficient on-column DNA removal. • High quality RNA for enhanced RT-PCR performance.

方便快速地从血清和血浆中分离小分子RNA和DNA. 样品体积300ul，重复上样900ul Isolation of small RNA and DNA from plasma and serum • Efficient and selective isolation of small RNA from plasma or serum samples • Superior recovery of small RNA > 15 nt • Optional on-column DNA digest for increased sensitivity in downstream applications • Simple and fast procedure – without phenol / chloroform

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等。 Mini spin kit for the isolation of RNA of highest integrity . • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion; • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included; • Up to 70 μg ready-to-use RNA; • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set; • Also available for QIAcube®.

从微量样品如单细胞、活检样品中提取高浓缩的RNA。<5 mg组织, 1-100000个细胞。操作简单，RNA回收率高，完整性好，洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。 Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA; • Isolation of RNA from small sample quantities like biopsy material or single cells; • Excellent RNA recovery and integrity; • Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL; • rDNase included for on-column DNA removal; • Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included; • High quality RNA, ready to use for RT-PCR and other applications.

通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化，柱上进行DNA消化可有效去除DNA污染，还可平行纯化基因组DNA。柱载量200ug，洗脱体积40-120ul，适用于组织，培养细胞，细菌和酵母等。 Mini spin kit for the isolation of RNA of highest integrity . • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion; • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included; • Up to 70 μg ready-to-use RNA; • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set; • Also available for QIAcube®.

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (500 µg) reliably purifies up to 500 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nt).

biotechrabbit™ GenUP Blood RNA Kit is designed for fast isolation of total RNA from up to 1 ml whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. Erythrocytes are removed in an initial lysis step. After an second lysis step, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. Including lysis, isolated RNA is available in approximately 45 min. The isolated RNA is suitable for a wide range of different molecular biology applications, including RT-PCR. The GenUP Blood RNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP Total RNA Kit (BR07009), GenUP Virus RNA Kit (BR07010), GenUP Virus DNA/RNA Kit (BR07011) or UPzol RNA Isolation Solution (BR07001).

biotechrabbit™ GenUP Total RNA Kit has been specially developed for a quick and easy purification of total RNA from eukaryotic cell suspensions, tissues and biopsies, Gram-negative (e.g., E. coli) and Gram-positive bacteria and other sources. After few initial procedures, the RNA is bound to a filter, washed and then eluted in a separate tube. DNA is removed physically by binding to a filter without any DNAse treatment or the use of toxic β‑mercaptoethanol. The purified RNA is ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis and others.

细胞或者组织RNA稳定剂，保持核酸的完整性。≤ 24 hours at 37 °C ≤ 7 days at 18–25 °C ≤ 1 month at 4 °C Long term at -20 °C or lower