The Monarch RNA Cleanup Wash Buffer, a component of the Monarch RNA Cleanup Kits (NEB #T2030, T2040, T2050), is used to wash RNA after binding to ensure the removal of contaminants such as enzyme, NTPs, and short RNA fragments. It is supplied as a 5X concentrate and requires dilution with ethanol (≥95%) before use. The product is supplied in 2 bottles, each with 20 ml of concentrated buffer.

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (50 µg) rapidly and reliably purifies up to 50 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (10 µg) rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (500 µg) reliably purifies up to 500 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nt).

氧钒核糖核苷复合物是通过 Berger 改良方法将四种 rNTP 的等摩尔混合物与氧化钒 IV 混合而成 。每一批复合物都要经过氧化钒 V 成分以及 RNase 活性抑制的检测。 氧钒复合物可用在 mRNA 的纯化过程中，作为外源 RNase 的抑制剂。也可在细胞裂解和蔗糖梯度细胞质成分分离过程中，抑制 RNase 活性。

The Monarch RNA Cleanup Binding Buffer, a component of the Monarch RNA Cleanup Kits (NEB #T2030, T2040, T2050), is a guanidine-based buffer designed to dilute the sample and prepare the RNA sample to bind to the silica columns.

The Monarch RNA Cleanup Columns (50 µg) are a component of the Monarch RNA Cleanup Kit (50 µg) (NEB #T2040) and can be used to purify up to 50 µg of RNA from enzymatic reactions including DNase treatment (NEB #M0303), labeling, and capping. The columns are ideal for cleaning up RNA after in vitro transcription reactions such as sgRNA synthesis reactions (NEB #E3322). The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 20 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and transfection.

The Monarch RNA Cleanup Columns (10 µg) are a component of the Monarch RNA Cleanup Kit (10 µg) (NEB #T2030) and can be used to purify up to 10 µg of RNA from enzymatic reactions. The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 6 μl and is ready for use in a variety of downstream applications including RT-PCR, RNA library prep for NGS, and labelling.

该产品是用于从细胞裂解液和组织中小规模分离 mRNA 的亲和基质。此分离是通过 Oligo d(T)25 与大部分真核生物 mRNA 中携带的 ploy(A) 形成共价杂交耦联实现的。既可用于细胞裂解后 mRNA 的直接分离，也可用于从总 RNA 中进行第二轮纯化。磁珠分离技术还能放大体系并将分离后的 mRNA 终体积进行调整，从而得到较小体积的完整RNA 洗脱液，不需要再从洗脱液中沉淀 poly(A)+ 转录物。磁珠能再生 3 次，实验者可选择将分离到的 mRNA 洗脱或直接以结合 mRNA 上的 d(T)25 DNA 为引物来进行 cDNA 链合成。

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (10 µg) rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (50 µg) rapidly and reliably purifies up to 50 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

链霉亲和素磁珠由 1 μm 超顺磁微粒与高纯度链霉亲和素共价结合而成。磁珠可用于捕获生物素标记的底物，包括生物素标记的抗原、抗体和核酸。由于生物素-链霉亲和素的相互作用很强，并且链霉亲和素的非特异性结合率很低，被捕获的底物可完全满足后续实验要求，包括 mRNA 的分离和一抗、二抗的获得。

NEB 的 mRNA 磁性分离试剂盒可用于从细胞或组织中分离完整的 poly(A)+ RNA，此过程不需要酚和其它有机溶剂。该技术的原理是将 Oligo d(T)25 与 1 μm 顺磁性磁珠耦联后，以此为固相支持物直接结合 poly(A)+ RNA。利用此方法可以进行多个样本的分离，也适用于自动化高通量分离。此外，磁性分离技术可得到较小体积的完整 RNA 洗脱液，不需要再从洗脱液中沉淀 poly(A)+ 转录物。因此在 1 小时内就能分离得到体现原始样品中 mRNA 分布特性的完整 poly(A)+ RNA。Oligo d(T)25 磁珠可再生 3 次，实验者可选择将分离到的 mRNA 洗脱下来或直接以结合于 mRNA 上的 dT DNA 为引物来进行 cDNA 链合成。

The Monarch RNA Cleanup Columns (500 µg) are a component of the Monarch RNA Cleanup Kit (500 µg) (NEB #T2050) and can be used to purify up to 500 µg of RNA from enzymatic reactions including high-yield RNA synthesis reactions. The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 50 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and transfection.

EpiMark® N6 甲基腺嘌呤富集试剂盒包含：一个兔源单克隆抗体和两组对照 RNA。抗体能特异性结合 N6 甲基腺嘌呤（m6A），其中一组对照 RNA 带有 m6A 修饰（Gaussia 荧光素酶），另一组对照 RNA 无 m6A 修饰 (Cypridina 荧光素酶)。对照 Gluc RNA 是由20% m6ATP 和 80% ATP 转录生成的，用来监测富集效果。

该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (500 µg) reliably purifies up to 500 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nt).

细胞或者组织RNA稳定剂，保持核酸的完整性。≤ 24 hours at 37 °C ≤ 7 days at 18–25 °C ≤ 1 month at 4 °C Long term at -20 °C or lower

快速高效地从血浆/血清样本中提取外泌体。 Exosome Precipitation Solutions – fast and efficient enrichment of exosomes from blood serum and plasma samples • Most efficient enrichment of exosomes for any downstream application; • Simple and fast precipitation of exosomes – avoid tedious ultra centrifugation; • Flexibility in sample amount – procedure can be scaled up or down depending on sample volume.

快速高效地从尿液样本中提取外泌体。 Exosome Precipitation Solutions – fast and efficient enrichment of exosomes from urine samples • Most efficient enrichment of exosomes for any downstream application; • Simple and fast precipitation of exosomes – avoid tedious ultra centrifugation; • Flexibility in sample amount – procedure can be scaled up or down depending on sample volume.

采用离子交换技术，提取高质量的RNA，更多400ug。 Anion-exchange chromatography – extra high purity for up to 400 μg RNA; • Ultra-pure RNA from different samples; • Separate isolation of different RNA species (tRNA, rRNA, mRNA) possible; • Separate elution of genomic DNA.

配套NucleoZOL试剂使用的RNA纯化柱，含buffer。 Combine NucleoZOL with proven NucleoSpin® technology;   • Total RNA (incl. miRNA) with a simple bind-wash-elute procedure; • Superior RNA yields due to the efficient NucleoZOL* lysis; • Save time and benefit from the easy and proven handling.

简单快速纯化或浓缩各种RNA样品。不含裂解试剂。柱载量200ug Simple, fast, and convenient clean-up of RNA; • Complete removal of RT-PCR inhibitors; • Time-saving procedure based on NucleoSpin® RNA II, without DNase digestions and homogenization steps; • RNA clean-up from pre-purified RNA (phenol/chloroform), enzymatic reactions (e.g. amplification reactions, labeling reactions.

快速高效浓缩纯化各种RNA样品。柱载量110ug，洗脱体积5-30ul. Highly efficient clean-up and concentration of RNA samples; • Complete removal of RT-PCR inhibitors; • Very high RNA recovery and concentration; • RNA clean-up from pre-purified RNA (phenol / chloroform), enzymatic reactions (e.g., amplification reactions, labeling reactions, DNase digestions). • Time-saving procedure based on NucleoSpin® RNA XS technology. • Input volume up to 300 μL • Elution in as little as 5 μL possible

快速从血浆中提取游离DNA，独特主体设计，可将洗脱体积减少到5ul. 更大上样量 720ul. For rapid purification of circulating DNA from plasma and serum; • High recovery even of fragmented DNA > 50 bp; • Elution in as little as 5 μL; • Concentrated DNA even from dilute samples; • DNA ready to use for real-time PCR.

采用单相提取技术，在使用时无需加入氯仿。NucleoZOL能够从各方面改善试剂法抽提RNA的操作体验，同时得到更好的抽提效果。 1.无需氯仿，无需分相，操作简单； 2.更少DNA/protein污染，更少的苯酚残留，RNA质量高； 3.适合多种样本(动植物细胞、组织、体液；酵母；细菌），RNA得率高；室温离心； 4.可以同时分离miRNA和长片段RNA 。

快速简洁地从1.3ml全血中提取高质量的RNA。无需预处理红细胞，产量高，无DNA污染，兼容常规采血管抗凝剂。 Midi spin kit for the isolation of RNA from fresh or frozen blood (human or animal); • Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysis at 4 °C – complete processing at room temperature); • Superior RNA yield and quality from up to 1.3 mL whole blood; • Efficient on-column DNA removal for an increased sensitivity in downstream applications; • Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin).

从组织和细胞中快速提取6-700ug的更完整的RNA。离心柱式，载量达700ug。提供NucleoSpin® Filters Midi (shredders)有效降低裂解液粘稠度以均质化。提供rDNase实现纯化柱上的DNA降解，有效去除DNA污染。 Midi spin kit for the isolation of RNA of highest integrity; • Efficient removal of genomic DNA – rDNase included for on-column digestion; • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters Midi (shredders) included; • Up to 600 μg ready-to-use RNA.

Absolutely RNA Lysis Buffer

简单快速提取植物样本中RNA，柱上消化gDNA。 Plant RNA mini spin kit – for all kinds of plant material. • Efficient removal of contaminating DNA – rDNase included for on-column digestion; • Efficient sample homogenization and reduction of viscosity –NucleoSpin® Filters (shredders) included; • Up to 70 μg ready-to-use RNA; • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set.