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                基因商城首页 > 试剂 > 分子生物学 > 核酸提取纯化 > RNA提取纯化

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                Monarch® RNA纯化洗液:Monarch RNA Cleanup Wash Buffer, 40 ml (2 x 20 ml of 5x concentrate) Kit

                价格:¥

                货号:T2042L
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                Monarch® RNA纯化试剂盒(10 µg):Monarch RNA Cleanup Kit (10 µg) 10 preps Kit

                价格:¥

                货号:T2030S
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                Monarch® RNA纯化试剂盒(50 µg):Monarch RNA Cleanup Kit (50 µg), 10 preps Kit

                价格:¥

                货号:T2040S
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                Monarch® RNA纯化试剂盒(500 µg):Monarch RNA Cleanup Kit (500 µg), 10 preps Kit

                价格:¥

                货号:T2050S
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                氧钒核糖核苷复合物:Ribonucleoside-Vanadyl Complex Kit

                价格:¥

                货号:S1402S
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                氧钒核糖核苷复合物是通过 Berger 改良方法将四种 rNTP 的等摩尔混合物与氧化钒 IV 混合而成(1)。每一批复合物都要经过氧化钒 V 成分以及 RNase 活性抑制的检测。氧钒复合物可用在 mRNA 的纯化过程中,作为外源 RNase 的抑制剂。也可在细胞裂解和蔗糖梯度细胞质成分分离过程中,抑制 RNase 活性。10 ml (200 mM)

                Monarch® RNA 纯化结合缓冲液:Monarch® RNA Cleanup Binding Buffer , 80 ml Kit

                价格:¥

                货号:T2041L
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                Monarch® RNA纯化柱(10 µg):Monarch RNA Cleanup Columns (10 µg) 100 columns + collection tubes Kit

                价格:¥

                货号:T2037L
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                Monarch® RNA纯化柱(50 µg):Monarch RNA Cleanup Columns (50 µg) , 100 columns + collection tubes Kit

                价格:¥

                货号:T2047L
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                Oligo d(T)25 磁珠:Oligo d(T)25 Magnetic Beads Kit

                价格:¥

                货号:S1419S
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                用于从细胞裂解液和组织中小规模分离mRNA的亲和基质。此分离是通过 Oligo d(T)25与大部分真核生物mRNA中携带的ploy(A)形成共价杂交耦联实现的。既可用于细胞裂解后mRNA的直接分离,也可用于从总RNA中进行第二轮纯化。磁珠分离技术还能放大体系并将分离后的mRNA终体积进行调整,从而得到较小体积的完整RNA 洗脱液,不需要再从洗脱液中沉淀 poly(A)+ 转录物。磁珠能再生3次,实验者可选择将分离到的mRNA洗脱或直接以结合mRNA上的d(T)25 DNA为引物来进行cDNA 链合成。1 mg Oligo d(T)25能结合10 μg poly(A)+ RNA。

                Monarch® RNA纯化试剂盒(50 µg):Monarch RNA Cleanup Kit (50 µg), 100 preps Kit

                价格:¥

                货号:T2040L
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                Monarch® RNA纯化试剂盒(10 µg):Monarch RNA Cleanup Kit (10 µg) 100 preps Kit

                价格:¥

                货号:T2030L
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                链霉亲和素磁珠:Streptavidin Magnetic Beads Kit

                价格:¥

                货号:S1420S
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                链霉亲和素磁珠由1μm超顺磁微粒与高纯度链霉亲和素共价结合而成。磁珠可用于捕获生物素标记的底物,包括生物素标记的抗原、抗体和核酸。由于生物素-链霉亲和素的相互作用很强,并且链霉亲和素的非特异性结合率很低,被捕获的底物可完全满足后续实验要求,包括mRNA的分离和一抗、二抗的获得。1 μm无孔超顺磁微粒(#S1420)1 mg S1420可结合 1000 pmol以上的游离生物素,或500 pmol以上的生物素标记的20 bp单链寡核苷酸。

                mRNA 磁性分离试剂盒:Magnetic mRNA Isolation Kit Kit

                价格:¥

                货号:S1550S
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                用于从细胞或组织中分离完整的poly(A)+RNA,不需酚和其它有机溶剂。将Oligo d(T)25与1 μm顺磁性磁珠耦联后,以此为固相支持物直接结合poly(A)+ RNA。可进行多个样本的分离,自动化高通量分离。磁性分离技术可得到较小体积的完整RNA洗脱液,不需要再从洗脱液中沉淀 poly(A)+ 转录物。因此在 1 小时内就能分离得到体现原始样品中mRNA分布特性的完整poly(A)+ RNA。Oligo d(T)25 磁珠可再生3次,实验者可选择将分离到的 mRNA 洗脱下来或直接以结合于mRNA上的dT DNA 为引物来进行cDNA 链合成。

                Monarch® RNA纯化柱(500 µg):Monarch® RNA Cleanup Columns (500 µg) , 100 columns + collection tubes Kit

                价格:¥

                货号:T2057L
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                EpiMark® N6 甲基腺嘌呤富集试剂盒:EpiMark N6-Methyladenosine Enrichment Kit  Kit

                价格:¥

                货号:E1610S
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                Monarch® RNA纯化试剂盒(500 µg):Monarch RNA Cleanup Kit (500 µg), 100 preps Kit

                价格:¥

                货号:T2050L
                品牌:NEB

                经销:基因生物技术国际贸易(上海)有限公司

                血浆/血清外泌体提取试剂:Exosome Precipitation Solution (Plasma) 2ml

                价格:¥

                货号:740398.2
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                从血清或血浆样本中抽提外泌体RNA;需配合NucleoSpin@miRNA Plasma Kit 配合使用

                尿液外泌体提取试剂:Exosome Precipitation Solution (Urine) 12ml

                价格:¥

                货号:740399.12
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                从微量FFPE样本中抽提总RNA,小于5mgFFPE样本

                NucleoSpin RNA Blood Midi (2) 1Pack

                价格:¥

                货号:740210.2
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                NucleoBond RNA/DNA Combi Sample (4) 1Pack

                价格:¥

                货号:740653.MUSTER
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                NucleoSpin 微量血浆游离 DNA 提取试剂盒:NucleoSpin Plasma XS 10 preps

                价格:¥

                货号:740900.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                Ideal for rapid purification of circulating DNA from serum and plasma
                • High recovery even of fragmented DNA > 50 bp
                • Elution in as little as 5 μL
                • Concentrated DNA even from dilute samples
                • DNA ready to use for real-time PCR

                Technology: Silica-membrane technology
                Format: XS spin columns – XS design
                Sample material: 720 µL plasma or serum (multiple loading steps)
                Fragment size: > 50 bp
                Typical yield: 0.1–100 ng per mL plasma, depending on sample
                Typical concentration: 1.2–12000 pg/µL, depending on sample
                Elution volume: 5–30 µL
                Preparation time High sensitivity procedure: 22–27 min/6 preps
                Rapid procedure: 15–20 min/6 preps

                Applications*
                • Circulating DNA from plasma or serum
                • Ideal for analysis of fetal DNA from maternal plasma
                • Ideal for detection of tumor markers in plasma
                NucleoSpin® Plasma XS Columns with Collection Tubes, Collection Tubes (2 mL), buffers, Proteinase K
                10 preps for the isolation of circulating DNA from plasma - NucleoSpin Plasma XS Columns, Collection Tubes, buffers, Proteinase K

                NucleoSpin 微量 RNA 纯化试剂盒:NucleoSpin RNA Clean-up XS 10 preps

                价格:¥

                货号:740903.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                快速高效浓缩纯化各种RNA样品。柱载量110ug,洗脱体积5-30ul
                Highly efficient clean-up and concentration of RNA samples
                • Complete removal of RT-PCR inhibitors
                • Very high RNA recovery and concentration
                • RNA clean-up from pre-purified RNA (phenol / chloroform), enzymatic reactions (e.g., amplification reactions, labeling reactions, DNase digestions)
                • Time-saving procedure based on NucleoSpin® RNA XS technology
                • Input volume up to 300 μL
                • Elution in as little as 5 μL possible

                Technology: Silica-membrane technology
                Format: XS spin columns
                Sample material: ≤ 300 µL RNA solution containing ≤ 90 µg RNA
                Fragment size: > 200 nt
                Typical recovery: 85–95%
                A260/A280: 1.9–2.1
                Typical RIN (RNA integrity number): Depending on sample quality (no significant loss of RIN detected)
                Elution volume: 5–30 µL
                Preparation time: 20 min/6 preps
                Binding capacity 110 µg

                Applications* RNA clean-up of
                • Pre-purified RNA (e.g., with TRIzol®)
                • DNase digestions (e.g., with MACHEREY-NAGEL rDNase Set)
                • Reaction mixtures
                • Up to 55-fold increase in concentration
                • Typical downstream applications: enzymatic labeling reactions, RT-PCR, DNA/RNA-based chip hybridization
                NucleoSpin® RNA XS Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers
                10 preps for the clean-up of total RNA - NucleoSpin RNA XS Columns, Collection Tubes, buffers

                NucleoSpin RNA 纯化试剂盒:NucleoSpin RNA Clean-up 10 preps

                价格:¥

                货号:740948.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                简单快速纯化或浓缩各种RNA样品。不含裂解试剂。柱载量200ug
                Simple, fast, and convenient clean-up of RNA
                • Complete removal of RT-PCR inhibitors
                • Time-saving procedure based on NucleoSpin® RNA II, without DNase digestions and homogenization steps
                • RNA clean-up from pre-purified RNA (phenol/chloroform), enzymatic reactions (e.g. amplification reactions, labeling reactions

                Technology: Silica-membrane technology
                Format: Mini spin columns
                Sample material: ≤ 200 µL phenol/chloroform extract or reaction mixture
                Fragment size: > 200 nt
                Typical recovery: 85–95%
                A260/A280: 1.9–2.1
                Typical RIN (RNA integrity number): Depending on sample quality (no significant loss of RIN detected)
                Elution volume: 40–120 µL
                Preparation time: 20 min/6 preps
                Binding capacity: 200 µg
                NucleoSpin® RNA II Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers
                10 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA II Columns, Collection Tubes, buffers

                NucleoSpin RNA Midi (2) 1Pack

                价格:¥

                货号:740962.2
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                NucleoSpin RNA Plus XS 10次

                价格:¥

                货号:740990.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                NucleoSpin 微量组织 RNA 提取试剂盒:NucleoSpin RNA XS 10 preps

                价格:¥

                货号:740902.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                从微量样品如单细胞、活检样品中提取高浓缩的RNA。<5 mg组织, 1-100000个细胞。操作简单,RNA回收率高,完整性好,洗脱体积仅需5ul。提供NucleoSpin® Filters (shredders)有效降低裂解液粘度和均质化。

                Purification of highly concentrated RNA from smallest samples. 5 µL elution volume → highly concentrated RNA
                • Isolation of RNA from small sample quantities like biopsy material or single cells
                • Excellent RNA recovery and integrity
                • Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
                • rDNase included for on-column DNA removal
                • Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
                • High quality RNA, ready to use for RT-PCR and other applications

                Technology: Silica-membrane technology
                Format: Mini spin column – XS design
                Sample material: Small amounts of tissue < 5 mg, 1-100000 cultured cells
                Fragment size: > 200 b
                Typical yield: 100 HeLa cells: 0.1–1.5 ng; 1000 HeLa cells: 10–15 ng
                A260/A280: 1.9–2.1
                Typical RIN (RNA integrity number): > 9 (depending on sample quality)
                Elution volume: 5–30 µL
                Preparation time: 40 min/12 preps
                Binding capacity: 110 µg

                Applications*
                • Total RNA isolation from cultured cells
                • Total RNA isolation from tissue
                • Total RNA isolation from cryosections
                • Total RNA isolation from laser captured cells
                • Total RNA isolation from small amounts of plant material
                • Total RNA isolation from samples stored in RNAlater®
                • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
                RNase protection assays
                NucleoSpin® RNA XS Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP
                10 preps for the isolation of total RNA - NucleoSpin RNA XS Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP

                NucleoSpin totalRNA FFPE XS (10) 1Pack

                价格:¥

                货号:740969.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                NucleoSpin RNA 提取试剂盒:NucleoSpin RNA 10 preps

                价格:¥

                货号:740955.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                通用快速离心柱式RNA提取试剂盒。保持RNA完整。提供NucleoSpin® Filters (shredders)方便裂解液的均质化,柱上进行DNA消化可有效去除DNA污染,还可平行纯化基因组DNA。柱载量200ug,洗脱体积40-120ul,适用于组织,培养细胞,细菌和酵母等
                Mini spin kit for the isolation of RNA of highest integrity
                • Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
                • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
                • Up to 70 μg ready-to-use RNA
                • Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set
                • Also available for QIAcube®

                Technology: Silica-membrane technology
                Format: Mini spin columns
                Sample material: < 5 x 10(6) cultured cells, < 10(9) bacterial cells, < 10(8) yeast cells, < 30 mg tissue
                Fragment size: > 200 b
                Typical yield: 14 μg from 106 HeLa cells, 70 μg from 109 bacterial cells
                A260/A280: 1.9–2.1
                Typical RIN (RNA integrity number): > 9
                Elution volume: 40–120 µL
                Preparation time: 30 min/6 preps
                Binding capacity: 200 µg

                Applications**
                • Total RNA isolation from cultured cells, tissue (standard protocol)
                • Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
                • Support protocol for total RNA from ≤ 100 μL biological fluids
                • Support protocol for RNA clean-up from reaction mixtures
                • Support protocol for total RNA from samples stored in RNAlater®
                • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
                RNase protection assays

                * The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
                – the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
                – decreasing PCR amplicon size
                NucleoSpin RNA® Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase
                10 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

                NucleoSpin 血浆miRNA 提取试剂盒:NucleoSpin miRNA Plasma 10 preps

                价格:¥

                货号:740981.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                方便快速地从血清和血浆中分离小分子RNA和DNA. 样品体积300ul,重复上样900ul
                Isolation of small RNA and DNA from plasma and serum
                • Efficient and selective isolation of small RNA from plasma or serum samples
                • Superior recovery of small RNA > 15 nt
                • Optional on-column DNA digest for increased sensitivity in downstream applications
                • Simple and fast procedure – without phenol / chloroform

                Technology: Silica-membrane technology
                Format: Mini spin columns
                Sample material: 300 µL plasma or serum (up to 900 µL with multiple loading)
                Fragment size: < 1000 nt / bp
                Elution volume: 20–50 µL
                Preparation time: 40 min/10 preps (without rDNase digestion); 70 min/10 preps (with rDNase digestion)
                Binding capacity: 200 µg

                Applications**
                • Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)
                • Typical downstream applications: real-time qRT-PCR, chip hybridisations
                NucleoSpin® miRNA Columns, Collection Tubes(2 mL), buffers, RNase-free rDNase
                10 preps for the isolation of small RNA and DNA from plasma - NucleoSpin miRNA Columns, Collection Tubes, buffers, RNase-free rDNase

                NucleoSpin FFPE 样本 RNA 提取试剂盒:NucleoSpin totalRNA FFPE 10 preps

                价格:¥

                货号:740982.10
                品牌:MNG

                经销:基因生物技术国际贸易(上海)有限公司

                一盒两用,可提取小RNA及大RNA,适用于FFPE样本

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