Protino® Ni-NTA 琼脂糖，用于His-Tag 蛋白纯化 Protino® Ni-NTA Agarose; • Aqueous suspension; • For batch binding, gravity flow, and FPLC™ – highest flexibility of applications; • Empty Protino® Columns for gravity flow available.

Protino® Glutathione Agarose 4B, best choice for cost-effective GST-tag protein purification; • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns; • Simply replace your current products, no optimization or protocol change necessary; • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use; • Market-leading performance for an unbeatable price; Protino® Glutathione Agarose 4B; • Aqueous suspension; • For batch binding, gravity flow, and FPLC™ – highest flexibility of applications; • Empty Protino® columns for gravity flow available;

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

噬菌体 M13 衍生噬菌体载体

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

Protino Ni-TED镍柱专用树脂， Protino® Ni-TED, the pure alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies). • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature ; Protino® Ni-TED Resin • Dry bulk material; • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility; • Batch procedure, gravity-flow column chromatography, FPLC™ applications; • Empty Protino® columns available for use with Protino® Ni-TED Resin; • Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin.

Protino Ni-IVD镍柱专用树脂， Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites; • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature. Protino® Ni-IDA Resin; • Dry bulk material; • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility; • Batch procedure, gravity-flow column chromatography, FPLC™ applications; • Empty Protino® columns available for use with Protino® Ni-IDA Resin; • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin.

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。 Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification; • Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies); • High capacity and high affinity; • Simply replace your current Ni-NTA products, no optimization or protocol change necessary; • Purification under native and denaturing conditions; • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use; • Market-leading performance for an unbeatable price ; Protino® Ni-NTA Columns; • Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.

Protino® Glutathione Agarose 4B, best choice for cost-effective GST-tag protein purification • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns • Simply replace your current products, no optimization or protocol change necessary • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use • Market-leading performance for an unbeatable price Protino® GST/4B Columns • Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B

抗 CBD 单克隆抗体

纯化融合蛋白的亲和基质（结合容量 = 2 mg/ml）

常用克隆载体

常用克隆载体

噬菌体 M13 衍生噬菌体载体

M13KO7 是 gII 基因具有 Met40IIe 突变的 M13 噬菌体。M13KO7 具有 P15A 的复制起点和 Tn903 卡那霉素抗性基因，这两个片段插入 M13 的复制起点。M13KO7 在没有噬菌粒 DNA 的条件下也能复制。当存在一个携带有野生型的 M13 或 f1 复制起点的噬菌粒时，单链噬菌粒可以完整包装，并分泌到培养基中。该特性可用于生产用作突变和测序的单链噬菌粒 DNA（在说明书中提供操作方法）。

一种亲和介质，能少量分离纯化与 CBD（Chitin Binding Domain）融合的目的蛋白。由于该磁珠有一个磁力核心，因此可以从细胞培养上清液中磁力分离与 CBD 融合的蛋白。再生之后磁珠不会影响结合能力。随后，被磁珠结合的融合蛋白可以从细胞裂解液中捕获与之发生相互作用的靶蛋白。

Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。 Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification; • Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies); • High capacity and high affinity; • Simply replace your current Ni-NTA products, no optimization or protocol change necessary; • Purification under native and denaturing conditions; • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use; • Market-leading performance for an unbeatable price ; Protino® Ni-NTA Columns; • Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.

纯化融合蛋白的亲和基质（结合容量 = 2 mg/ml）

pTXB1 是大肠杆菌表达载体，携带有一个小的蛋白自剪切元件即来自 Mycobacterium xenopi gyrA 基因的 mini 内含肽［Mxe GyrA 内含肽，22 kDa］。蛋白自剪切元件经修饰后与来自芽孢杆菌（Bacillus circulans）的几丁质结合域（CBD）一起共同形成一个内含肽标签。该标签可与几丁质珠（NEB #S6651）结合。使用硫醇试剂（如 DTT）或 MESNA（2-mercaptoethanesulfonic acid）（后续连接用）诱导内含肽自我剪切并释放出目的蛋白。

pTYB21 的内含肽标签包含酿酒酵母（Sce）VMA1 内含肽和几丁质结合域融合在目的蛋白的 N 末端。

Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。 Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification; • Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies); • High capacity and high affinity; • Simply replace your current Ni-NTA products, no optimization or protocol change necessary; • Purification under native and denaturing conditions; • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use; • Market-leading performance for an unbeatable price ; Protino® Ni-NTA Columns; • Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.

His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6×His-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators (10 µM). The binding of His-tags is stoichiometric and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA). biotechrabbit™ Tris-NTA is available with a free amine group or conjugated to biotin. It can be used in a large range of applications, including protein detection and labeling, coupling proteins, lipids or cells to surfaces, protein purification and reversible protein modification.