ExcelTaq DNA 聚合酶 (ExcelTaq DNA polymerase, TP1000)，是一种重组的热稳定DNA 聚合酶，这个基因是从耐热菌中克隆并在大肠杆菌经诱导表达后，再分离纯化得到。 该酶具高效率合成DNA之活性以及热稳定性，适合对例行性或特殊的基因进行扩增反应。ExcelTaq DNA Polymerase is a recombinant thermo-stable DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With high DNA synthesis rate and thermo-stability, ExcelTaq DNA Polymerase is suitable for general and specialized PCR applications.
ExcelTaq 5X PCR 预混试剂盒 (ExcelTaq 5X PCR Master Mix, TP1100) 为一组直接使用于扩增目标DNA片段的产品。 此产品设计的目的为提供给有PCR应用需求的用户可立即使用，不需额外准备其他试剂。 这组PCR反应预混液内含大部分PCR实验所需之成分，用户只需另外加入模板和特异性引物。 本产品节省许多操作实验时间，同时也减少吸取液体次数，进而降低操作所产生的误差。 本PCR预混试剂盒含有五倍立即使用预混液并附赠六倍DNA上样指示染剂(蓝)，其中五倍即用型混合液包含Taq DNA聚合酶、反应缓冲液、氯化镁、dNTsP和酶稳定剂；而DNA上样指示染剂 (蓝)含有二种追踪染剂 (Xylene cyanol FF 与 Bromophenol blue)可用于观察DNA片段在琼脂醣凝胶中的大略位置。The ExcelTaq 5× PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Mix is supplied as a 5× concentrated master mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, 200 μM dNTPs, enzyme stabilizer.
biotechrabbit™ Taq DNA polymerase is a first-choice enzyme for all routine and molecular diagnostics PCR applications . The exceptional quality and purity of the enzyme ensures highest performance that is utilized by the diagnostics industry and research labs. The polymerase is suitable for standard and fast PCR applications giving high product yields from various templates with targets of up to 3 kb in size.
Taq DNA polymerase is a thermostable, highly processive 5'-3' DNA polymerase that has low 5'- 3' exonuclease activity and lacks 3'-5' exonuclease (proofreading) activity. The latter allows incorporation of modified nucleotides.
The enzyme also exhibits deoxynucleotidyl transferase activity that results in the addition of extra A overhang at the 3'-ends of PCR products, allowing easy cloning of PCR products into vectors with T overhangs.
ExcelTaq 5X PCR 预混染剂试剂盒(ExcelTaq 5X PCR Master Dye Mix, TP1200)为一组直接使用于扩增目标DNA片段的产品。 此产品设计的目的为提供给有PCR应用需求的用户立即使用，不需额外准备其他试剂。 这组PCR反应混合液包含所有进行PCR所需的成分，用户只需另外加入模板和特异性引物。 这不仅节省了许多实验时间，同时也减少吸取液体次数进而降低操作所产生的误差。 本PCR预混试剂盒为五倍立即使用混合液，包含Taq DNA聚合酶、反应缓冲液、氯化镁、dNTPs、酶稳定剂和电泳追踪染剂(Bromophenol blue)，此PCR产物可直接上样于琼脂醣凝胶中，进行电泳试验。The ExcelTaq 5× PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5× concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA Polymerase, reaction buffer, MgSO4, 200 μM dNTP, enzyme stabilizer and PCR friendly loading dye solution containing tracking dyes (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent- loading dye master mix conveniently.
ExcelTaq 5X PCR 预染荧光试剂盒(ExcelTaq 5X Fluorescent PCR Master Mix, TP1260)为一组可立即使用于扩增目标DNA片段的产品。 此试剂被设计为即用型预染试剂，适用于所有之PCR应用。 ExcelTaq 5倍PCR预染荧光试剂盒提供五倍浓缩即用型试剂除模板及引物外，包含了所有进行PCR时所需要之成分。 此外，预染试剂中亦包含了电泳追踪染剂（Bromophenol blue）及DNA荧光染剂，因此可使操作者于电泳过程中，搭配蓝光观测仪或紫外光观测仪实时追踪电泳过程并省略DNA染色所需之时间。 PCR反应产物可直接上样于1 倍TAE或TBE 缓冲液中进行电泳侦测。The ExcelTaq 5× Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The ExcelTaq 5× Fluorescent PCR Master Mix is supplied as a 5× concentrated ready-to-use mixture containing all the essential ingredients for PCR with the exception of template and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue), as well as a safer fluorescent DNA staining dye which enables the user to track the electrophoresis process in real time as well as eliminating the need for staining process. The resultant PCR reaction mixture is sufficiently dense to be loaded directly into 1×TAE or 1×TBE buffer for electrophoresis.
OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentR™ DNA 聚合酶的混合物，可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´→5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低，都只需更小的优化即能获得更高的产量。
biotechrabbit™ YourTaq PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq PCR Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
The 2× YourTaq PCR Mix contains pure biotechrabbit YourTaq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
biotechrabbit™ YourTaq Direct-Load PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. YourTaq Hot Start PCR Mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. The mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
The 2× YourTaq Direct-Load PCR Mix contains pure biotechrabbit YourTaq DNA Polymerase, extremely high-quality dNTPs, two dyes (blue and yellow) that separate during electrophoresis, allowing migration progress to be monitored, and sufficient buffer density for direct loading onto agarose gels. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
biotechrabbit™ Taq DNA polymerase is a first-choice enzyme for all routine and molecular diagnostics PCR applications . The exceptional quality and purity of the enzyme ensures highest performance that is utilized by the diagnostics industry and research labs. The polymerase is suitable for standard and fast PCR applications giving high product yields from various templates with targets of up to 3 kb in size.
Taq DNA polymerase is a thermostable, highly processive 5'-3' DNA polymerase that has low 5'- 3' exonuclease activity and lacks 3'-5' exonuclease (proofreading) activity. The latter allows incorporation of modified nucleotides.
The enzyme also exhibits deoxynucleotidyl transferase activity that results in the addition of extra A overhang at the 3'-ends of PCR products, allowing easy cloning of PCR products into vectors with T overhangs.
OneTaq DNA 聚合酶是经过优化的 Taq DNA 聚合酶与 Deep VentR™ DNA 聚合酶的混合物，可用于常规与复杂的 PCR 实验。Deep Vent DNA 聚合酶的 3´→5´ 外切酶活性提升了 Taq DNA 聚合酶的保真性和聚合能力。OneTaq 反应缓冲液与 High GC Enhancer 的配方使得无论模板的 GC 含量高低，都只需更小的优化即能获得更高的产量。
biotechrabbit™ YourTaq PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq PCR Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
The 2× YourTaq PCR Mix contains pure biotechrabbit YourTaq DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
biotechrabbit™ YourTaq Direct-Load PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. YourTaq Hot Start PCR Mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. The mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
The 2× YourTaq Direct-Load PCR Mix contains pure biotechrabbit YourTaq DNA Polymerase, extremely high-quality dNTPs, two dyes (blue and yellow) that separate during electrophoresis, allowing migration progress to be monitored, and sufficient buffer density for direct loading onto agarose gels. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
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