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                  基因商城首页 > 试剂 > 分子生物学 > PCR/RT-PCR > 热启动PCR

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                  不限 Biotechrabbit GmbH Bioline Agilent SMOBIO LGC Genomics Ltd NEB
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                  2X 多重 PCR热启动预混液, 50 μl体系(50 rxn):2X Multiplex PCR Hot-Start Master Mix 50 reactions of 50 µl 50 rxn of 50 μl

                  价格:

                  货号:BR0200801
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  Biotechrabbit 2X 多重热启动PCR mix适用于多重终点PCR ,独特优化的缓冲液组成,单次反应可实现10种以上的靶标在50bp 至2kb快速扩增,目前已有测试到560重以上,是超多重PCR的理想选择。Mix中包含Enhangcers可有效扩增低丰度和高GC含量模板优化mix帮助快速建立反应。
                  特点:
                  专用性:配方经过优化,以达到超多重PCR最佳效果;
                  热启动:高灵敏度和特异性;
                  通用性:在多种多重模板表现良好。

                  biotechrabbit™ Multiplex PCR Master Mix is a perfect choice for endpoint multiplex PCR. The unique buffer composition is optimized for robust simultaneous amplification of 10 or more targets from 50 bp – 2 kb in a single reaction. The Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized Multiplex PCR buffer; thus, only template, PCR primers and PCR-grade water are added. The enhancers included in the mix enable efficient amplification of low abundant or GC-rich templates. Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. The hot-start efficiently minimizes primer–dimers and mispriming.

                  ApStarTaq™ DNA聚合酶:ApStarTaq™ Hot-Start DNA Polymerase 5 U/µl 250 Units 5 U/μl, 250 Units

                  价格:

                  货号:BR0201501
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit ApStarTaq™ DNA Polymerase is an aptamer based hot-start enzyme and the first-choice for fast PCR reactions. The exceptional quality and purity of the enzyme ensures highest performance, suitable for standard and fast PCR cycling in both, endpoint and real-time assays. It ensures high product yields with low background and without primer–dimer formation or non-specific priming. The aptamer binds to Taq DNA Polymerase and inhibits the enzyme activity at temperatures below 45°C. This ensures full hot-start functionality. The enzyme is released during standard PCR cycling conditions. There is no need for separate heating or denaturation steps, allowing fast PCR reactions.

                  YourTaq™热启动DNA聚合酶:YourTaq™ Hot-Start DNA Polymerase 5 U/µl 250 Units 5 U/μl, 250 Units

                  价格:

                  货号:BR0202101
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit™ YourTaq Hot Start DNA Polymerase is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start DNA Polymerase is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. YourTaq Hot Start DNA Polymerase is a highly pure enzyme. Together with the optimized buffer and high-quality dNTPs (BR0600202) a mix is achieved for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).

                  热启动Taq DNA聚合酶(野生型):Hot-Start Taq DNA Polymerase 5 U/µl 500 Units 5 U/μl, 500 Units

                  价格:

                  货号:BR0200102
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit™ Hot Start Taq DNA Polymerase is a first-choice hot-start PCR enzyme for all demanding PCR applications. The enzyme ensures high product yields with low background and without primer–dimer formation and nonspecific priming. The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps. The optional use of 5× PCR Enhancer improves PCR results in many cases, including impure template or low template abundance.

                  热启动 Taq 2X Master Mix:Hot Start Taq 2X Master Mix 100 reactions (50 ul vol) 100 rxns

                  价格:

                  货号:M0496S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  为了加样方便,Taq 与热启动 Taq DNA 聚合酶均有预混液剂型可供选择。Taq 2X Master Mix 还有 Quick-Load 的形式,可用于直接凝胶上样。Taq PCR 试剂盒包含 Taq DNA 聚合酶、dNTP 混合液、缓冲液、MgCl2 和Quick-Load 2-Log DNA Ladder。

                  OneTaq® 热启动 2X Master Mix(提供标准缓冲液):OneTaq Hot Start 2X Master Mix with Standard Buffer 100 reactions (50 ul vol) 100 rxns

                  价格:

                  货号:M0484S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  为了加样方便,OneTaq DNA 聚合酶与 OneTaq 热启动 DNA 聚合酶均有预混液剂型。预混液有标准或 GC 缓冲液可供选择,High GC Enhancer 随 GC 缓冲液一同提供。此外这些预混液还有 Quick-Load 的形式,可用于直接凝胶上样。

                  OneTaq® 热启动 2X Master Mix(提供 GC 缓冲液):OneTaq Hot Start 2X Master Mix with GC Buffer 100 reactions (50 ul vol) 100 rxns

                  价格:

                  货号:M0485S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  为了加样方便,OneTaq DNA 聚合酶与 OneTaq 热启动 DNA 聚合酶均有预混液剂型。预混液有标准或 GC 缓冲液可供选择,High GC Enhancer 随 GC 缓冲液一同提供。此外这些预混液还有 Quick-Load 的形式,可用于直接凝胶上样。

                  OneTaq® 热启动 Quick-Load® 2X Master Mix(提供标准缓冲液):OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer 100 reactions (50 ul vol) 100 rxns

                  价格:

                  货号:M0488S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  为了加样方便,OneTaq DNA 聚合酶与 OneTaq 热启动 DNA 聚合酶均有预混液剂型。预混液有标准或 GC 缓冲液可供选择,High GC Enhancer 随 GC 缓冲液一同提供。此外这些预混液还有 Quick-Load 的形式,可用于直接凝胶上样。

                  OneTaq® 热启动 Quick-Load 2X Master Mix(提供 GC 缓冲液):OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer 100 reactions (50 ul vol) 100 rxns

                  价格:

                  货号:M0489S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  为了加样方便,OneTaq DNA 聚合酶与 OneTaq 热启动 DNA 聚合酶均有预混液剂型。预混液有标准或 GC 缓冲液可供选择,High GC Enhancer 随 GC 缓冲液一同提供。此外这些预混液还有 Quick-Load 的形式,可用于直接凝胶上样。

                  热启动 Taq DNA 聚合酶:Hot Start Taq DNA Polymerase 200 units 200 units

                  价格:

                  货号:M0495S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  超高的性价比、引人注目的商业宣传,NEB 的热启动 Taq 是分子诊断和其它应用的理想选择。与化学修饰或基于抗体结合的热启动聚合酶不同,NEB 的热启动 Taq 利用一种基于核酸适配体(aptamer)技术。这种独特的核酸适配体通过非共价键作用与聚合酶结合,从而抑制聚合酶在非许可温度下的聚合反应。这种新方法不需要激活步骤,减少样品降解的可能性、缩短整个反应时间。

                  OneTaq® 热启动 DNA 聚合酶:OneTaq Hot Start DNA Polymerase 200 units 200 units

                  价格:

                  货号:M0481S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  OneTaq 热启动 DNA 聚合酶利用核酸适配体(aptamer)作为抑制剂,不仅使用方便,而且减少了引物二聚体和其他次级产物对反应的干扰。这种核酸适配体抑制剂能够与聚合酶发生可逆结合,当温度低于 45℃ 时抑制聚合酶活性,因而能够在室温下建立反应。在正常的热循环条件下 OneTaq 热启动 DNA 聚合酶即能被激活,无需额外的高温启动步骤。因此 OneTaq 热启动 DNA 聚合酶能够替换常规的或现有的基于 Taq DNA 聚合酶的 PCR 反应。

                  YourTaq™热启动PCR预混液,2X:2X YourTaq™ Hot-Start PCR Master Mix 200 reactions of 50 µl 200 rxn of 50 μl

                  价格:

                  货号:BR0202201
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit™ YourTaq Hot Start PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start PCR Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. The 2× YourTaq Hot Start PCR Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).

                  YourTaq™快速上样热启动PCR预混液,2X:2X YourTaq™ Direct-Load Hot-Start PCR Master Mix 200 reactions of 50 µl 200 rxn of 50 μl

                  价格:

                  货号:BR0202301
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit™ YourTaq Direct-Load Hot Start PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. YourTaq Hot Start PCR Mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. The mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. The 2× YourTaq Direct-Load Hot Start PCR Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs, two dyes (blue and yellow) that separate during electrophoresis, allowing migration progress to be monitored, and sufficient buffer density for direct loading onto agarose gels. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).

                  LongAmp® 热启动 Taq DNA 聚合酶:LongAmp Hot Start Taq DNA Polymerase 500 units 500 units

                  价格:

                  货号:M0534S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  LongAmp 热启动 Taq DNA 聚合酶利用了一种特定合成的核酸适配体,它能够与酶可逆结合,当温度低于45℃ 时抑制聚合酶活性,正常的热循环条件下释放聚合酶。

                  YourTaq™热启动DNA聚合酶:YourTaq™ Hot-Start DNA Polymerase 5 U/µl 1250 Units 5 U/μl, 1250 Units

                  价格:

                  货号:BR0202102
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit™ YourTaq Hot Start DNA Polymerase is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start DNA Polymerase is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. YourTaq Hot Start DNA Polymerase is a highly pure enzyme. Together with the optimized buffer and high-quality dNTPs (BR0600202) a mix is achieved for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).

                  2X 多重 PCR热启动预混液, 50 μl体系(250 rxn):2X Multiplex PCR Hot-Start Master Mix 250 reactions of 50 µl 250 rxn of 50 μl