biotechrabbit™ Multiplex PCR Master Mix is a perfect choice for endpoint multiplex PCR. The unique buffer composition is optimized for robust simultaneous amplification of 10 or more targets from 50 bp – 2 kb in a single reaction.
The Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized Multiplex PCR buffer; thus, only template, PCR primers and PCR-grade water are added. The enhancers included in the mix enable efficient amplification of low abundant or GC-rich templates.
Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. The hot-start efficiently minimizes primer–dimers and mispriming.
biotechrabbit ApStarTaq™ DNA Polymerase is an aptamer based hot-start enzyme and the first-choice for fast PCR reactions. The exceptional quality and purity of the enzyme ensures highest performance, suitable for standard and fast PCR cycling in both, endpoint and real-time assays. It ensures high product yields with low background and without primer–dimer formation or non-specific priming.
The aptamer binds to Taq DNA Polymerase and inhibits the enzyme activity at temperatures below 45°C. This ensures full hot-start functionality. The enzyme is released during standard PCR cycling conditions. There is no need for separate heating or denaturation steps, allowing fast PCR reactions.
biotechrabbit™ YourTaq Hot Start DNA Polymerase is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start DNA Polymerase is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
YourTaq Hot Start DNA Polymerase is a highly pure enzyme. Together with the optimized buffer and high-quality dNTPs (BR0600202) a mix is achieved for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
biotechrabbit™ Hot Start Taq DNA Polymerase is a first-choice hot-start PCR enzyme for all demanding PCR applications. The enzyme ensures high product yields with low background and without primer–dimer formation and nonspecific priming.
The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps.
The optional use of 5× PCR Enhancer improves PCR results in many cases, including impure template or low template abundance.
OneTaq 热启动 DNA 聚合酶利用核酸适配体（aptamer）作为抑制剂，不仅使用方便，而且减少了引物二聚体和其他次级产物对反应的干扰。这种核酸适配体抑制剂能够与聚合酶发生可逆结合，当温度低于 45℃ 时抑制聚合酶活性，因而能够在室温下建立反应。在正常的热循环条件下 OneTaq 热启动 DNA 聚合酶即能被激活，无需额外的高温启动步骤。因此 OneTaq 热启动 DNA 聚合酶能够替换常规的或现有的基于 Taq DNA 聚合酶的 PCR 反应。
biotechrabbit™ YourTaq Hot Start PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start PCR Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
The 2× YourTaq Hot Start PCR Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
biotechrabbit™ YourTaq Direct-Load Hot Start PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. YourTaq Hot Start PCR Mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. The mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
The 2× YourTaq Direct-Load Hot Start PCR Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs, two dyes (blue and yellow) that separate during electrophoresis, allowing migration progress to be monitored, and sufficient buffer density for direct loading onto agarose gels. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
biotechrabbit™ YourTaq Hot Start DNA Polymerase is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start DNA Polymerase is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
YourTaq Hot Start DNA Polymerase is a highly pure enzyme. Together with the optimized buffer and high-quality dNTPs (BR0600202) a mix is achieved for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
biotechrabbit™ Multiplex PCR Master Mix is a perfect choice for endpoint multiplex PCR. The unique buffer composition is optimized for robust simultaneous amplification of 10 or more targets from 50 bp – 2 kb in a single reaction.
The Master Mix contains pure biotechrabbit Hot Start Taq DNA Polymerase, extremely high-quality dNTPs and optimized Multiplex PCR buffer; thus, only template, PCR primers and PCR-grade water are added. The enhancers included in the mix enable efficient amplification of low abundant or GC-rich templates.
Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody, which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. The hot-start efficiently minimizes primer–dimers and mispriming.
biotechrabbit ApStarTaq™ DNA Polymerase is an aptamer based hot-start enzyme and the first-choice for fast PCR reactions. The exceptional quality and purity of the enzyme ensures highest performance, suitable for standard and fast PCR cycling in both, endpoint and real-time assays. It ensures high product yields with low background and without primer–dimer formation or non-specific priming.
The aptamer binds to Taq DNA Polymerase and inhibits the enzyme activity at temperatures below 45°C. This ensures full hot-start functionality. The enzyme is released during standard PCR cycling conditions. There is no need for separate heating or denaturation steps, allowing fast PCR reactions.
biotechrabbit™ Hot Start Taq DNA Polymerase is a first-choice hot-start PCR enzyme for all demanding PCR applications. The enzyme ensures high product yields with low background and without primer–dimer formation and nonspecific priming.
The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody which is quickly released at elevated temperatures, ensuring the enzyme is active only during PCR. There is no need for prolonged heating or denaturation steps.
The optional use of 5× PCR Enhancer improves PCR results in many cases, including impure template or low template abundance.
biotechrabbit™ YourTaq Hot Start PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start PCR Mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
The 2× YourTaq Hot Start PCR Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
biotechrabbit™ YourTaq Direct-Load Hot Start PCR Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. YourTaq Hot Start PCR Mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. The mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
The 2× YourTaq Direct-Load Hot Start PCR Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs, two dyes (blue and yellow) that separate during electrophoresis, allowing migration progress to be monitored, and sufficient buffer density for direct loading onto agarose gels. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
OneTaq 热启动 DNA 聚合酶利用核酸适配体（aptamer）作为抑制剂，不仅使用方便，而且减少了引物二聚体和其他次级产物对反应的干扰。这种核酸适配体抑制剂能够与聚合酶发生可逆结合，当温度低于 45℃ 时抑制聚合酶活性，因而能够在室温下建立反应。在正常的热循环条件下 OneTaq 热启动 DNA 聚合酶即能被激活，无需额外的高温启动步骤。因此 OneTaq 热启动 DNA 聚合酶能够替换常规的或现有的基于 Taq DNA 聚合酶的 PCR 反应。
biotechrabbit™ YourTaq Hot Start DNA Polymerase is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Hot Start DNA Polymerase is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.
YourTaq Hot Start DNA Polymerase is a highly pure enzyme. Together with the optimized buffer and high-quality dNTPs (BR0600202) a mix is achieved for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).
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