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                  基因商城首页 > 试剂 > 分子生物学 > PCR/RT-PCR > cDNA一链合成试剂盒

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                  产品名/货号/品牌 简介 包装  
                  LunaScript RT SuperMix Kit 25 reactions 1Kit

                  价格:

                  货号:E3010S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  ProtoScript II cDNA 第一链合成试剂盒 :ProtoScript II First Strand cDNA Synthesis Kit 30 reactions 30 rxns

                  价格:

                  货号:E6560S
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  ProtoScript II cDNA 链合成试剂盒含有两种经过优化的混合液,ProtoScript II 酶混合液与 ProtoScript II 反应混合液。酶混合液含 ProtoScript II 反转录酶和小鼠 RNase 抑制剂;反应混合液含 dNTPs 与经过优化的缓冲液。ProtoScript II 反转录酶是重组的 M-MuLV 反转录酶,降低了 RNase H 活性且提高了热稳定性。与野生型 M-MuLV 反转录酶相比,ProtoScript II 可在更高的温度下合成链 cDNA。该酶活性温度高达50℃,且具有特异性高、cDNA 产量高的特点。

                  LunaScript™反转录试剂盒:LunaScript RT SuperMix Kit 100 reactions 100次

                  价格:

                  货号:E3010L
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  LunaScript SuperMix 反转录试剂盒是经过优化的预混液,用于两步法 RT–qPCR 的第一步 cDNA 第一链合成。该预混液最具特色的是采用了耐热 Luna 反转录酶,可在高温下合成 cDNA。预混液中的小鼠 RNase 抑制剂可保护模板 RNA 不被降解。在预混液中同时含有 6 碱基随机引物和 poly-dT 引物,可覆盖全长目标 RNA。 此外,LunaScript SuperMix 反转录试剂盒含有可视蓝色示踪染料,在两步法 RT–qPCR 实验中起到提示作用。无论起始量是高达 1 μg 的总 RNA,还是低到单拷贝的总 RNA,使用该试剂盒进行反转录,都能获得稳定的、线性的和高灵敏度的检测结果。 Luna+Q20:S20Script RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, the LunaScript RT SuperMix contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The LunaScript RT SuperMix offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.

                  ProtoScript II cDNA 第一链合成试剂盒 :ProtoScript II First Strand cDNA Synthesis Kit 150 reactions 150 rxns

                  价格:

                  货号:E6560L
                  品牌:NEB

                  经销:基因生物技术国际贸易(上海)有限公司

                  ProtoScript II cDNA 链合成试剂盒含有两种经过优化的混合液,ProtoScript II 酶混合液与 ProtoScript II 反应混合液。酶混合液含 ProtoScript II 反转录酶和小鼠 RNase 抑制剂;反应混合液含 dNTPs 与经过优化的缓冲液。ProtoScript II 反转录酶是重组的 M-MuLV 反转录酶,降低了 RNase H 活性且提高了热稳定性。与野生型 M-MuLV 反转录酶相比,ProtoScript II 可在更高的温度下合成链 cDNA。该酶活性温度高达50℃,且具有特异性高、cDNA 产量高的特点。

                  cDNA合成试剂盒:cDNA Synthesis Kit 10 reactions of 20 µl 10 rxn of 20 μl

                  价格:

                  货号:BR0400401
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit™ cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. biotechrabbit RevertUP™ II Reverse Transcriptase enables highly efficient reverse transcription with increased thermostability. biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb. For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.

                  逆转录试剂盒:ExcelRTTM Reverse Transcription kit 100 Rxn

                  价格:

                  货号:RP1300
                  品牌:SMOBIO

                  经销:基因生物技术国际贸易(上海)有限公司

                  ExcelRT™ 反转录试剂盒是一个完整、 高效、 简便的试剂盒,可合成高质量的条互补DNA链 (cDNA)。试剂盒包含可在37~50°C稳定合成条互补DNA链 (cDNA)的逆转录酶。从莫罗尼鼠白血病病毒(Moloney murine leukemin virus)的逆转录酶(简称M-MLV)重组改良而来,降低了核糖核酸酶H (RNase H)活性,增加其热稳定性。试剂盒还包括RNAok™ Rnase抑制剂,可以有效抵抗 RNase A, RNase B, and RNase C。同时提供 oligo (dT)20和随机引物,分别用来合成以 poly(A)结尾的mRNA 和 Totoal RNA的cDNA链。ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively. 

                  逆转录试剂盒 II:ExcelRTTM Reverse Transcription kit II 100 Rxn

                  价格:

                  货号:RP1400
                  品牌:SMOBIO

                  经销:基因生物技术国际贸易(上海)有限公司

                  ExcelRT™ 反转录试剂盒是一个完整、 高效、 简便的试剂盒,可合成高质量的条互补DNA链 (cDNA)。试剂盒包含可在37~50°C稳定合成条互补DNA链 (cDNA)的逆转录酶。从莫罗尼鼠白血病病毒(Moloney murine leukemin virus)的逆转录酶(简称M-MLV)重组改良而来,降低了核糖核酸酶H (RNase H)活性,增加其热稳定性。试剂盒还包括RNAok™ Rnase抑制剂,可以有效抵抗 RNase A, RNase B, and RNase C。同时提供优化的RT缓冲液,oligo (dT)20和随机引物的混合物,用来高效合成适合进行实时定量PCR的短链 cDNA。ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR. 

                  cDNA合成试剂盒:cDNA Synthesis Kit 125 reactions of 20 µl 125 rxn of 20 μl

                  价格:

                  货号:BR0400403
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit™ cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. biotechrabbit RevertUP™ II Reverse Transcriptase enables highly efficient reverse transcription with increased thermostability. biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb. For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.

                  cDNA合成试剂盒:cDNA Synthesis Kit 250 reactions of 20 µl 250 rxn of 20 μl

                  价格:

                  货号:BR0400404
                  品牌:Biotechrabbit GmbH

                  经销:基因生物技术国际贸易(上海)有限公司

                  biotechrabbit™ cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. biotechrabbit RevertUP™ II Reverse Transcriptase enables highly efficient reverse transcription with increased thermostability. biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb. For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.

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