ProtoScript II cDNA 链合成试剂盒含有两种经过优化的混合液，ProtoScript II 酶混合液与 ProtoScript II 反应混合液。酶混合液含 ProtoScript II 反转录酶和小鼠 RNase 抑制剂；反应混合液含 dNTPs 与经过优化的缓冲液。ProtoScript II 反转录酶是重组的 M-MuLV 反转录酶，降低了 RNase H 活性且提高了热稳定性。与野生型 M-MuLV 反转录酶相比，ProtoScript II 可在更高的温度下合成链 cDNA。该酶活性温度高达50℃，且具有特异性高、cDNA 产量高的特点。

LunaScript SuperMix 反转录试剂盒是经过优化的预混液，用于两步法 RT–qPCR 的第一步 cDNA 第一链合成。该预混液最具特色的是采用了耐热 Luna 反转录酶，可在高温下合成 cDNA。预混液中的小鼠 RNase 抑制剂可保护模板 RNA 不被降解。在预混液中同时含有 6 碱基随机引物和 poly-dT 引物，可覆盖全长目标 RNA。 此外，LunaScript SuperMix 反转录试剂盒含有可视蓝色示踪染料，在两步法 RT–qPCR 实验中起到提示作用。无论起始量是高达 1 μg 的总 RNA，还是低到单拷贝的总 RNA，使用该试剂盒进行反转录，都能获得稳定的、线性的和高灵敏度的检测结果。 Luna+Q20:S20Script RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, the LunaScript RT SuperMix contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The LunaScript RT SuperMix offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.

ProtoScript II cDNA 链合成试剂盒含有两种经过优化的混合液，ProtoScript II 酶混合液与 ProtoScript II 反应混合液。酶混合液含 ProtoScript II 反转录酶和小鼠 RNase 抑制剂；反应混合液含 dNTPs 与经过优化的缓冲液。ProtoScript II 反转录酶是重组的 M-MuLV 反转录酶，降低了 RNase H 活性且提高了热稳定性。与野生型 M-MuLV 反转录酶相比，ProtoScript II 可在更高的温度下合成链 cDNA。该酶活性温度高达50℃，且具有特异性高、cDNA 产量高的特点。

biotechrabbit™ cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. biotechrabbit RevertUP™ II Reverse Transcriptase enables highly efficient reverse transcription with increased thermostability. biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb. For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.

The RNAMaxx transcription kit uses T7 RNA polymerase to synthesize RNA from a double-stranded DNA template containing the T7 promoter. To generate high RNA yields, high concentrations of ribonucleotides are added to the reaction mixture. When a ribonucleotide is incorporated into RNA, a molecule of pyrophosphate is released. High concentrations of pyrophosphate have been shown to inhibit further polymerization. To remove this inhibition, yeast inorganic pyrophosphatase has been added to the reaction.1 The use of a higher concentration of ribonucleotides and the addition of yeast inorganic pyrophosphatase in the reaction mixture increases the RNA yield 10 to 30 times the yield produced by traditional in vitro transcription methods.

Easy-A high-fidelity cloning enzymes combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

AffinityScript Multiple Temperature Reverse Transcriptase (RT) produces high cDNA yields over the broadest temperature range with high affinity to primer/template complexes. It delivers high cDNA yield when amplifying low RNA input amounts and resolving RNA secondary structure using temperatures up to 55°C. Robust cDNA yield even with GC-rich sequences Increased activity at all temperatures, from 37-55°C Ideal sensitivity in detecting low input RNA Synthesizes cDNA up to 20 kb For a limited time only, receive a 30% discount on Agilent SureCycler PCR instrument. In addition, receive a 30% discount on PCR reagents, enzymes, and kits. Download Agilent’s brand new 2018 PCR Catalog . View the complete list of products here. For customers in the United States, Canada, and Korea, use promo code "1161" For customers in European countries, use promo code "1156" For customers purchasing through distributors in selected countries, use promo code "1157" Note: Discount may vary for different regions. Contact your local distributors for additional details. For Research Use Only. Not for Use in Diagnostic Procedures.

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers the highest reverse transcription accuracy currently available. This new MMLV-derived RT generates cDNA with 3 to 6-fold fewer errors, while promoting full-length cDNA synthesis and superior RT-PCR performance. Proofreading activity reduces errors Synthesize cDNA with 3 to 6-fold fewer errors Achieve up to 8-fold better RT-PCR accuracy Up to 3x faster RT-PCR reaction times High yields of full length cDNA up to 20 kb For Research Use Only. Not for use in diagnostic procedures.

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

AccuScript High-Fidelity Reverse Transcriptase (RT) delivers 3.7x more accuracy than other commercially available reverse transcriptases and 6.6x more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high-fidelity, specificity, and throughput.

ExcelRT™ 反转录试剂盒是一个完整、 高效、 简便的试剂盒，可合成高质量的条互补DNA链 (cDNA)。试剂盒包含可在37~50°C稳定合成条互补DNA链 (cDNA)的逆转录酶。从莫罗尼鼠白血病病毒(Moloney murine leukemin virus)的逆转录酶(简称M-MLV)重组改良而来，降低了核糖核酸酶H (RNase H)活性，增加其热稳定性。试剂盒还包括RNAok™ Rnase抑制剂，可以有效抵抗 RNase A, RNase B, and RNase C。同时提供 oligo (dT)20和随机引物，分别用来合成以 poly(A)结尾的mRNA 和 Totoal RNA的cDNA链。ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively. 

ExcelRT™ 反转录试剂盒是一个完整、 高效、 简便的试剂盒，可合成高质量的条互补DNA链 (cDNA)。试剂盒包含可在37~50°C稳定合成条互补DNA链 (cDNA)的逆转录酶。从莫罗尼鼠白血病病毒(Moloney murine leukemin virus)的逆转录酶(简称M-MLV)重组改良而来，降低了核糖核酸酶H (RNase H)活性，增加其热稳定性。试剂盒还包括RNAok™ Rnase抑制剂，可以有效抵抗 RNase A, RNase B, and RNase C。同时提供优化的RT缓冲液，oligo (dT)20和随机引物的混合物，用来高效合成适合进行实时定量PCR的短链 cDNA。ExcelRT™ Reverse Transcription Kit II is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with optimized RT Buffer and Oligo (dT)/Random Primer Mix for highly efficient synthesis of short chain cDNA suitable for real-time PCR. 

AffinityScript Multiple Temperature Reverse Transcriptase (RT) produces high cDNA yields over the broadest temperature range with high affinity to primer/template complexes. It delivers high cDNA yield when amplifying low RNA input amounts and resolving RNA secondary structure using temperatures up to 55°C.

biotechrabbit™ cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. biotechrabbit RevertUP™ II Reverse Transcriptase enables highly efficient reverse transcription with increased thermostability. biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb. For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.

biotechrabbit™ cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. biotechrabbit RevertUP™ II Reverse Transcriptase enables highly efficient reverse transcription with increased thermostability. biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb. For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.