ExcelRT一步法RT-PCR试剂盒 (ExcelRT One-Step RT-PCR Kit, RP1100),可在同一个反应管内一步完成逆转录与PCR反应实验,利用RNA作为模板以目标基因的正向和反向引物合成cDNA及进行PCR反应。 本产品为两倍预混(Premix)反应试剂,内含优化的缓冲液、dNTPs、Mg2+、stabilizer,及预混的逆转录酶与常规型DNA Taq酶,提供用户一个便捷的实验方式,且本产品仅需于PCR聚合酶反应器 (thermal cycler) 内设定一个程序依序进行逆转录与PCR反应,即便是微量的RNA也可被检测出来。The ExcelRTTM One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target gene from either total RNA or mRNA. The ExcelRTTM One-Step RT-PCR Kit provides users an alternative to two lengthy steps (first strand generation and amplification) by using a single mixture in a single tube for the one-step reaction. The ExcelRTTM One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+, enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRTTM One-Step RT-PCR Kit allows users to complete the RT-PCR process using a thermocycler in a single reaction setting and is ideal for target RNA amplification. This product is capable of detecting even trace amounts of target RNA.
biotechrabbit One Step RT-PCR Mix预混液,快速便捷、高得率、高特异性,一步实现逆转录和扩增。高效热稳定逆转录酶和专有的 核糖核酸抑制剂的结合确保cDNA的高产出。这款经优化的预混液可以实现高度特异性检测,甚至是复杂模板。
biotechrabbit™ One Step RT-PCR Kit provides an easy and efficient way to perform both reverse transcription of RNA and PCR amplification of cDNA in one step. Only RNA template, primers and PCR-grade water are added.
The 20× RT-RI Blend, which contains a blend of an efficient thermostable reverse transcriptase and a proprietary Ribonuclease Inhibitor, ensures high yields of cDNA.
The 2× One Step Mix contains unique Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers in an optimized buffer and provides high PCR product yields with minimal background even when using low-abundance and difficult templates. PCR enhancers included in the mix allow efficient amplification of complex templates including GC or AT-rich sequences.
biotechrabbit One Step RT-PCR Mix预混液,快速便捷、高得率、高特异性,一步实现逆转录和扩增。高效热稳定逆转录酶和专有的 核糖核酸抑制剂的结合确保cDNA的高产出。这款经优化的预混液可以实现高度特异性检测,甚至是复杂模板。
biotechrabbit™ One Step RT-PCR Kit provides an easy and efficient way to perform both reverse transcription of RNA and PCR amplification of cDNA in one step. Only RNA template, primers and PCR-grade water are added.
The 20× RT-RI Blend, which contains a blend of an efficient thermostable reverse transcriptase and a proprietary Ribonuclease Inhibitor, ensures high yields of cDNA.
The 2× One Step Mix contains unique Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers in an optimized buffer and provides high PCR product yields with minimal background even when using low-abundance and difficult templates. PCR enhancers included in the mix allow efficient amplification of complex templates including GC or AT-rich sequences.