双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

分子信标是一类具有猝灭基团和荧光报道基团的双标记FRET探针。其与传统的双标记探针的区别在于分子信标在3'和5'末端掺入了一段短的（约5 bp）互补的茎序列。 这些互补序列杂交能够形成报告基团和猝灭基团在空间上紧靠在一起的“茎环”结构。这种替代构象使分子信标具有很强的分辨能力，能够识别仅具有单个核苷酸差异的不同目标序列。分子信标另一个不同于传统双标记探针之处在于它们不依赖于由Taq聚合酶的5'外切核酸酶活性引起探针水解产生荧光，因此，PCR后的溶解曲线是可能的。 就像标准双标记BHQ®探针一样，分子信标也进行多重定量。多重定量使用多个光谱分辨的荧光探针能够同时检测几个靶标。 LGC Biosearch分子信标得到了来自新泽西州纽瓦克的公共健康研究所许可进行销售。有关分子信标探针设计信息，请参阅公共健康研究所的分子信标网站。 Molecular Beacons are a type dual-labeled FRET probes incorporating a quencher and a fluorophore reporter molecule. They differ from traditional dual-labeled probes, due to the incorporation of a short (~5 b.p.) complementary stem sequence at the 3' and 5' ends. These complementary sequences hybridize to form a “stem-loop” structure which holds the reporter and quencher close together in space. This alternate conformation makes Molecular Beacons more discriminatory, allowing them to resolve different target sequences differing by a single nucleotide. Molecular Beacons also differ from traditional dual-labeled probes because they do not rely on probe hydrolysis from the 5' exonuclease activity of Taq polymerase to generate its fluorescence, therefore, a post-PCR melt curve is possible. Just like standard Dual-labeled BHQ® probes it is possible to multiplex assays using Molecular Beacons. Multiplexing allows for the detection of several targets simultaneously using multiple spectrally resolved fluorescent probes. Molecular Beacons are sold under license from the Public Health Research Institute, Newark, NJ. For information on designing Molecular Beacons please refer to The Public Health Research Institute's Molecular Beacons website.

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

BHQplus®探针是一种用于qPCR实验的新型探针，采用了双重稳定技术进行修饰，并用Biosearch的Black Hole Quencher®染料标记在探针末端进行淬灭。BHQplus探针与其他传统qPCR探针的区别在于其允许设计较短的寡核苷酸（通常长度为15-25个碱基），可用于检测更复杂困难的靶序列，特异性更强，是对单核苷酸多态性SNP进行基因分型的理想选择。 BHQplus探针提供两种形式：1）若需要BHQplus探针和引物分开单独包装，选择“BHQplus探针”；2）如果希望探针和引物混合在单管中，则选择“ValuMix Assay for SNP geneotyping”。 用于SNP基因分型的ValuMix试剂包含两个定制的BHQplus®探针和两个引物混合在一个单管中。这个混合mix形式能够更小化移液误差并简化实验流程，可直接用于SNP基因分型。 BHQplus探针特点： 小但强大：双稳态允许设计较短的双标记探针 高保真度：较短的序列特异性更强，能够确保错配杂交率更低 经过验证的性能：黑洞淬灭剂染料确保在目标杂交之前完全熄灭荧光 探针设计简便：通过Biosearch免费的在线工具RealTimeDesign™软件就可以设计得到BHQplus探针和引物 底线：基于探针的SNP基因分型的经济替代方法 请注意，长于25个碱基的序列可以被设计为传统探针，在B​​HQplus形式中可能不能正常工作。如果这样的序列已经具有70℃的预测熔融温度，则可以考虑订购BHQ®探针。相反，短于15个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且应该首先分析与BHQplus化学的相容性。有关探头设计的任何问题，请联系基因有限公司技术人员。 The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms. BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”. ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping. Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available. BHQplus probes feature: 1. Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes 2. High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch 3. Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization 4. A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign™ software 5. Bottom line: An economical alternative for probe-based SNP Genotyping Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB™) probe and should first be analyzed for compatibility with the BHQplus chemistry. Please contact info@genecompany.com with any questions regarding probe design. NOTES: Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Biosearch Technologies offers RealTimeDesign™ software, a FREE assay design program hosted on the web. Start designing your BHQplus probes and primer pairs today!

BHQplus®探针是一种用于qPCR实验的新型探针，采用了双重稳定技术进行修饰，并用Biosearch的Black Hole Quencher®染料标记在探针末端进行淬灭。BHQplus探针与其他传统qPCR探针的区别在于其允许设计较短的寡核苷酸（通常长度为15-25个碱基），可用于检测更复杂困难的靶序列，特异性更强，是对单核苷酸多态性SNP进行基因分型的理想选择。 BHQplus探针提供两种形式：1）若需要BHQplus探针和引物分开单独包装，选择“BHQplus探针”；2）如果希望探针和引物混合在单管中，则选择“ValuMix Assay for SNP geneotyping”。 用于SNP基因分型的ValuMix试剂包含两个定制的BHQplus®探针和两个引物混合在一个单管中。这个混合mix形式能够更小化移液误差并简化实验流程，可直接用于SNP基因分型。 BHQplus探针特点： 小但强大：双稳态允许设计较短的双标记探针 高保真度：较短的序列特异性更强，能够确保错配杂交率更低 经过验证的性能：黑洞淬灭剂染料确保在目标杂交之前完全熄灭荧光 探针设计简便：通过Biosearch免费的在线工具RealTimeDesign™软件就可以设计得到BHQplus探针和引物 底线：基于探针的SNP基因分型的经济替代方法 请注意，长于25个碱基的序列可以被设计为传统探针，在B​​HQplus形式中可能不能正常工作。如果这样的序列已经具有70℃的预测熔融温度，则可以考虑订购BHQ®探针。相反，短于15个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且应该首先分析与BHQplus化学的相容性。有关探头设计的任何问题，请联系基因有限公司技术人员。 The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms. BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”. ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping. Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available. BHQplus probes feature: 1. Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes 2. High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch 3. Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization 4. A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign™ software 5. Bottom line: An economical alternative for probe-based SNP Genotyping Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB™) probe and should first be analyzed for compatibility with the BHQplus chemistry. Please contact info@genecompany.com with any questions regarding probe design. NOTES: Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Biosearch Technologies offers RealTimeDesign™ software, a FREE assay design program hosted on the web. Start designing your BHQplus probes and primer pairs today!