用于PCR分析的BHQ（Black Hole Quencher，黑洞淬灭剂）标记的Scorpions引物跟双标记探针和分子信标探针不一样，其将引物和探针结合在一个分子里面，引物在3'末端，探针包含在5‘端发夹环结构中。 Scorpions引物利用单分子机制，在实时定量PCR用于瞬时荧光的溶液中能够更快地起作用。与分子信标类似，这些引物在PCR循环期间不需要酶切割探针。这些特点使Scorpions引物成为快速实时定量PCR，终点PCR，SNP检测和基因定量的重要工具。 相比其它PCR探针形式，Scorpion引物具有许多优势： 1. 很好的特异性 2. 极好的信噪比 3. 容易实现多重 蝎形结构：Scorpions引物是与其它FRET探针不同的探针/引物杂交体，其设计使得它们只有在扩增期间掺入PCR产物时才发光。这些引物结合了两个不同的结构：1）靶特异性DNA探针序列和2）靶特异性PCR引物。 DNA探针序列：DNA探针元件在3'和5'末端具有一段自身互补配对的茎序列，这段茎序列将目标特异性区域维持在中间的发夹状茎环中。荧光基团分子共价连接到5'端，黑暗猝灭剂（即黑洞猝灭剂BHQ染料）连在茎环结构的3'端。荧光基团和BHQ淬灭染料通过茎结构紧密相邻，确保在热循环之前的荧光能够被有效猝灭。 靶特异性引物：靶特异性PCR引物通过一个blocking moiety与DNA探针序列的3'端共价连接。该moiety能够阻止聚合酶延伸到探针元件中，从而避免由此造成的假阳性。 BHQ® (Black Hole Quencher®)-labeled Scorpions primers for PCR analysis are unlike dual-labeled probes and Molecular Beacons because they combine primer and probe in one molecule, with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end. Scorpions primers utilize a uni-molecular mechanism that acts faster in solution for instantaneous fluorescence in real-time PCR. Similar to Molecular Beacons, these primers do not require enzymatic cleavage of the probe during PCR cycling. These qualities make Scorpions primers valuable tools for rapid, real-time PCR, endpoint PCR, SNP detection, and gene quantification. Scorpions primers provide a number of important advantages over other PCR probe formats: Exceptional Specificity Excellent S:N Easily Multiplexed Scorpions Architecture: Scorpions primers are probe/primer hybrids unlike other FRET probes, whose design is such that they emit light only when incorporated into the PCR product during amplification. These primers incorporate two distinct structures: 1) a target-specific DNA probing sequence and 2) a target-specific PCR primer. DNA Probing Sequence: The DNA probing element incorporates a self-complementary stem sequence at the 3' and 5' ends that holds the target specific region in a hairpin loop. A fluorophore molecule is covalently linked to the 5' end and, the dark quencher, a Black Hole Quencher dye, is linked to the 3' end of the loop structure. The fluorophore and BHQ dye are held in close physical proximity by the stem structure to ensure efficient quenching prior to thermal cycling. Target-specific Primer: The target-specific PCR primer is covalently linked to the 3' end of the DNA probing sequence through a blocking moiety. This moiety prevents polymerase extension into the probing element which could manifest as false positives.

用于PCR分析的BHQ（Black Hole Quencher，黑洞淬灭剂）标记的Scorpions引物跟双标记探针和分子信标探针不一样，其将引物和探针结合在一个分子里面，引物在3'末端，探针包含在5‘端发夹环结构中。 Scorpions引物利用单分子机制，在实时定量PCR用于瞬时荧光的溶液中能够更快地起作用。与分子信标类似，这些引物在PCR循环期间不需要酶切割探针。这些特点使Scorpions引物成为快速实时定量PCR，终点PCR，SNP检测和基因定量的重要工具。 相比其它PCR探针形式，Scorpion引物具有许多优势： 1. 很好的特异性 2. 极好的信噪比 3. 容易实现多重 蝎形结构：Scorpions引物是与其它FRET探针不同的探针/引物杂交体，其设计使得它们只有在扩增期间掺入PCR产物时才发光。这些引物结合了两个不同的结构：1）靶特异性DNA探针序列和2）靶特异性PCR引物。 DNA探针序列：DNA探针元件在3'和5'末端具有一段自身互补配对的茎序列，这段茎序列将目标特异性区域维持在中间的发夹状茎环中。荧光基团分子共价连接到5'端，黑暗猝灭剂（即黑洞猝灭剂BHQ染料）连在茎环结构的3'端。荧光基团和BHQ淬灭染料通过茎结构紧密相邻，确保在热循环之前的荧光能够被有效猝灭。 靶特异性引物：靶特异性PCR引物通过一个blocking moiety与DNA探针序列的3'端共价连接。该moiety能够阻止聚合酶延伸到探针元件中，从而避免由此造成的假阳性。 BHQ® (Black Hole Quencher®)-labeled Scorpions primers for PCR analysis are unlike dual-labeled probes and Molecular Beacons because they combine primer and probe in one molecule, with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end. Scorpions primers utilize a uni-molecular mechanism that acts faster in solution for instantaneous fluorescence in real-time PCR. Similar to Molecular Beacons, these primers do not require enzymatic cleavage of the probe during PCR cycling. These qualities make Scorpions primers valuable tools for rapid, real-time PCR, endpoint PCR, SNP detection, and gene quantification. Scorpions primers provide a number of important advantages over other PCR probe formats: Exceptional Specificity Excellent S:N Easily Multiplexed Scorpions Architecture: Scorpions primers are probe/primer hybrids unlike other FRET probes, whose design is such that they emit light only when incorporated into the PCR product during amplification. These primers incorporate two distinct structures: 1) a target-specific DNA probing sequence and 2) a target-specific PCR primer. DNA Probing Sequence: The DNA probing element incorporates a self-complementary stem sequence at the 3' and 5' ends that holds the target specific region in a hairpin loop. A fluorophore molecule is covalently linked to the 5' end and, the dark quencher, a Black Hole Quencher dye, is linked to the 3' end of the loop structure. The fluorophore and BHQ dye are held in close physical proximity by the stem structure to ensure efficient quenching prior to thermal cycling. Target-specific Primer: The target-specific PCR primer is covalently linked to the 3' end of the DNA probing sequence through a blocking moiety. This moiety prevents polymerase extension into the probing element which could manifest as false positives.

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

CAL Fluor®和Quasar®染料校准标准品可用于改进需要光谱校准的qPCR仪中的信号解卷积。它们使仪器能够存储每种染料的荧光图谱并控制荧光之间的相互串扰。串扰是指某种荧光信号泄漏到相邻滤光器中的一种现象。多重定量中多个靶点同时扩增但独立检测，这种串扰问题尤其需要注意。不同荧光报告基团的信号代表不同靶标分子的表达量，使用标准染料进行校准能够解决仪器的光谱重叠问题。 染料校准标准与荧光基团一起共价连接到oligo-dT（10-mer）上，以更好地模拟荧光探针的信号。使用染料校准作为参考，分析软件会分析在扩增期间每个荧光基团预期发出的荧光，然后减去从不适当的滤光器组件中的信号值。因此，使用T10-染料标准品进行校准后减少了串扰的幅度。 CAL Fluor®和Quasar®染料标准品跨越宽范围的发射波长，因此在实验设计中具有更大的灵活性。有关常用仪器光谱校准的说明请参阅以下链接：https://biosearch-cdn.azureedge.net/assetsv6/BTI_Spectral_Calibration_Instructions.pdf。在某种仪器上，用于多重定量的所有染料必须一起校准，因此了LGC Biosearch也提供FAM校准标准染料。若您需要定制合成某种校准染料，或者如果您对校准程序有任何疑问，请咨询info@genecompany.com。 CAL Fluor® and Quasar® dye calibration standards are available to improve signal deconvolution in real-time qPCR thermal cyclers that require spectral calibration. They enable the instrument to store the fluorescent profile of each dye and control for crosstalk. Crosstalk is the bleed-through of signal into adjacent filter-sets that are oriented to detect other fluorophores. This crosstalk is particularly consequential to a multiplexed assay, since multiple targets are amplified simultaneously but detected independently. Different fluorescent reporters are used to signal each target and so calibration with dye standards allows the instrument to resolve their overlapping spectra. Dye calibration standards are formulated with the fluorophore covalently attached to an oligo-dT (10-mer), to better mimic the signal from a fluorescent probe. Using the dye calibration as a reference, the analysis software anticipates how much fluorescence to expect from each fluorophore during amplification, and will subtract out signal from inappropriate filter-sets. Calibration with the T10-dye standards therefore reduces the magnitude of crosstalk. CAL Fluor and Quasar dye standards span a wide range of emission wavelengths for maximum flexibility in experimental design. Instructions on spectral calibration are linked below for a number of common instruments. On certain instruments, all dyes that will be multiplexed together must be calibrated together, and so a FAM standard is available as well. Please inquire with info@genecompany.com to discuss the custom synthesis of any dye not currently available as a calibration standard, or if you have any questions on the calibration procedure.

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!

BHQplus®探针是一种用于qPCR实验的新型探针，采用了双重稳定技术进行修饰，并用Biosearch的Black Hole Quencher®染料标记在探针末端进行淬灭。BHQplus探针与其他传统qPCR探针的区别在于其允许设计较短的寡核苷酸（通常长度为15-25个碱基），可用于检测更复杂困难的靶序列，特异性更强，是对单核苷酸多态性SNP进行基因分型的理想选择。 BHQplus探针提供两种形式：1）若需要BHQplus探针和引物分开单独包装，选择“BHQplus探针”；2）如果希望探针和引物混合在单管中，则选择“ValuMix Assay for SNP geneotyping”。 用于SNP基因分型的ValuMix试剂包含两个定制的BHQplus®探针和两个引物混合在一个单管中。这个混合mix形式能够更小化移液误差并简化实验流程，可直接用于SNP基因分型。 BHQplus探针特点： 小但强大：双稳态允许设计较短的双标记探针 高保真度：较短的序列特异性更强，能够确保错配杂交率更低 经过验证的性能：黑洞淬灭剂染料确保在目标杂交之前完全熄灭荧光 探针设计简便：通过Biosearch免费的在线工具RealTimeDesign™软件就可以设计得到BHQplus探针和引物 底线：基于探针的SNP基因分型的经济替代方法 请注意，长于25个碱基的序列可以被设计为传统探针，在B​​HQplus形式中可能不能正常工作。如果这样的序列已经具有70℃的预测熔融温度，则可以考虑订购BHQ®探针。相反，短于15个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且应该首先分析与BHQplus化学的相容性。有关探头设计的任何问题，请联系基因有限公司技术人员。 The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms. BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”. ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping. Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available. BHQplus probes feature: 1. Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes 2. High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch 3. Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization 4. A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign™ software 5. Bottom line: An economical alternative for probe-based SNP Genotyping Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB™) probe and should first be analyzed for compatibility with the BHQplus chemistry. Please contact info@genecompany.com with any questions regarding probe design. NOTES: Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Biosearch Technologies offers RealTimeDesign™ software, a FREE assay design program hosted on the web. Start designing your BHQplus probes and primer pairs today!

BHQplus®探针是一种用于qPCR实验的新型探针，采用了双重稳定技术进行修饰，并用Biosearch的Black Hole Quencher®染料标记在探针末端进行淬灭。BHQplus探针与其他传统qPCR探针的区别在于其允许设计较短的寡核苷酸（通常长度为15-25个碱基），可用于检测更复杂困难的靶序列，特异性更强，是对单核苷酸多态性SNP进行基因分型的理想选择。 BHQplus探针提供两种形式：1）若需要BHQplus探针和引物分开单独包装，选择“BHQplus探针”；2）如果希望探针和引物混合在单管中，则选择“ValuMix Assay for SNP geneotyping”。 用于SNP基因分型的ValuMix试剂包含两个定制的BHQplus®探针和两个引物混合在一个单管中。这个混合mix形式能够更小化移液误差并简化实验流程，可直接用于SNP基因分型。 BHQplus探针特点： 小但强大：双稳态允许设计较短的双标记探针 高保真度：较短的序列特异性更强，能够确保错配杂交率更低 经过验证的性能：黑洞淬灭剂染料确保在目标杂交之前完全熄灭荧光 探针设计简便：通过Biosearch免费的在线工具RealTimeDesign™软件就可以设计得到BHQplus探针和引物 底线：基于探针的SNP基因分型的经济替代方法 请注意，长于25个碱基的序列可以被设计为传统探针，在B​​HQplus形式中可能不能正常工作。如果这样的序列已经具有70℃的预测熔融温度，则可以考虑订购BHQ®探针。相反，短于15个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且应该首先分析与BHQplus化学的相容性。有关探头设计的任何问题，请联系基因有限公司技术人员。 The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms. BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”. ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping. Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available. BHQplus probes feature: 1. Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes 2. High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch 3. Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization 4. A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign™ software 5. Bottom line: An economical alternative for probe-based SNP Genotyping Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB™) probe and should first be analyzed for compatibility with the BHQplus chemistry. Please contact info@genecompany.com with any questions regarding probe design. NOTES: Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Biosearch Technologies offers RealTimeDesign™ software, a FREE assay design program hosted on the web. Start designing your BHQplus probes and primer pairs today!

BHQplus®探针是一种用于qPCR实验的新型探针，采用了双重稳定技术进行修饰，并用Biosearch的Black Hole Quencher®染料标记在探针末端进行淬灭。BHQplus探针与其他传统qPCR探针的区别在于其允许设计较短的寡核苷酸（通常长度为15-25个碱基），可用于检测更复杂困难的靶序列，特异性更强，是对单核苷酸多态性SNP进行基因分型的理想选择。 BHQplus探针提供两种形式：1）若需要BHQplus探针和引物分开单独包装，选择“BHQplus探针”；2）如果希望探针和引物混合在单管中，则选择“ValuMix Assay for SNP geneotyping”。 用于SNP基因分型的ValuMix试剂包含两个定制的BHQplus®探针和两个引物混合在一个单管中。这个混合mix形式能够更小化移液误差并简化实验流程，可直接用于SNP基因分型。 BHQplus探针特点： 小但强大：双稳态允许设计较短的双标记探针 高保真度：较短的序列特异性更强，能够确保错配杂交率更低 经过验证的性能：黑洞淬灭剂染料确保在目标杂交之前完全熄灭荧光 探针设计简便：通过Biosearch免费的在线工具RealTimeDesign™软件就可以设计得到BHQplus探针和引物 底线：基于探针的SNP基因分型的经济替代方法 请注意，长于25个碱基的序列可以被设计为传统探针，在B​​HQplus形式中可能不能正常工作。如果这样的序列已经具有70℃的预测熔融温度，则可以考虑订购BHQ®探针。相反，短于15个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且应该首先分析与BHQplus化学的相容性。有关探头设计的任何问题，请联系基因有限公司技术人员。 The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms. BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”. ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping. Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available. BHQplus probes feature: 1. Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes 2. High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch 3. Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization 4. A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign™ software 5. Bottom line: An economical alternative for probe-based SNP Genotyping Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB™) probe and should first be analyzed for compatibility with the BHQplus chemistry. Please contact info@genecompany.com with any questions regarding probe design. NOTES: Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Biosearch Technologies offers RealTimeDesign™ software, a FREE assay design program hosted on the web. Start designing your BHQplus probes and primer pairs today!

双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!