噬菌体 M13 衍生噬菌体载体

常用克隆载体

常用克隆载体

噬菌体 M13 衍生噬菌体载体

pTXB1 是大肠杆菌表达载体，携带有一个小的蛋白自剪切元件即来自 Mycobacterium xenopi gyrA 基因的 mini 内含肽［Mxe GyrA 内含肽，22 kDa］。蛋白自剪切元件经修饰后与来自芽孢杆菌（Bacillus circulans）的几丁质结合域（CBD）一起共同形成一个内含肽标签。该标签可与几丁质珠（NEB #S6651）结合。使用硫醇试剂（如 DTT）或 MESNA（2-mercaptoethanesulfonic acid）（后续连接用）诱导内含肽自我剪切并释放出目的蛋白。

pTYB21 的内含肽标签包含酿酒酵母（Sce）VMA1 内含肽和几丁质结合域融合在目的蛋白的 N 末端。

常用克隆载体，用于分子生物学实验中的分子克隆研究。

常用克隆载体

The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners. Purify interacting proteins from a broader range of mammalian cells Recombinant adenoviruses can infect either dividing or non-dividing cells For Research Use Only. Not for Use in Diagnostic Procedures.

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-4 plasmid, this featurൊൎൗൗൠൣ൦൵൹ൿංඃඅඖකනඳරලලවෆ්්ාුූූෘෟෲ෴࿕࿘᱐᱙ᱚ᱿​‍‐‐–—‘‚“„†•……‰‰‹›⁄⁄₨₨€€₹₹™™−−∕∕∙∙∶∶◌◌●●☬☬꠰꠹

Determine the direct or indirect involvement of new gene products, growth factors, and drug candidates in specific pathways with our PathDetect Trans-Reporting Systems.  Easily investigate whether your protein or compound influences the activation of any of the six transcription factors offered.  These systems are available as plasmid vectors with a luciferase reporter for co-transfection with your gene of interest into the cell line of your choice.  For ultimate flexibility, the pFA-CMV vector includes a multiple cloning site for inserting and expressing any transcription factor of interest. The system also includes positive and negative controls that ensure the success of your experiments.

Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems.  When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element For ultimate flexibility, our pLuc-MCS expression vector includes a multiple cloning site to facilitate insertion of your enhancer or transcription element. The system also includes positive and negative controls that ensure the success of your experiments.

The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.

The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.

The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.

The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.

The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.

The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-2 plasmid, this featur龜Ȭ윰ൎ煮Ȭ저ൎﳈȬ죐ൎ︈Ȭ즠ൎˆȭ쩰ൎLjȭ럠ᆍ맘ȫ룀ᆍ뻨ȫᆍ꾈ȫ｀ᆍ꟠ȫᆎ

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-3 plasmid, this featurൊൎൗൗൠൣ൦൵൹ൿංඃඅඖකනඳරලලවෆ්්ාුූූෘෟෲ෴࿕࿘᱐᱙ᱚ᱿​‍‐‐–—‘‚“„†•……‰‰‹›⁄⁄₨₨€€₹₹™™−−∕∕∙∙∶∶◌◌●●☬☬꠰꠹

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).