Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 1000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-TED 1000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 1000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-IDA 1000 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 2000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-TED 2000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 2000 Packed Columns, buffers 5 preps for the purification of His-tag proteins - Protino Ni-IDA 2000 Packed Columns, buffers
Ni-TED纯化填料螯合Ted基团，可耐受高压高流速，可用于FPLC，高亲和高选择性得到更纯的His-tag蛋白，在还原和螯合试剂中亦能保持高度稳定，极低金属离子解离。干粉形式提供更易保存。有配套的空纯化柱和96孔纯化板提供，灵活多用。 High specificity for Purification of His-tag proteins, the purest alternative for His-tag protein purification • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-TED Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-TED Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin
Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples • High protein concentration • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-IDA Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-IDA Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin
Technology: IMAC, immobilized metal ion affinity chromatography Chelating ligand: IDA (iminodiacetic acid) Format: Bulk matrix Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Binding capacity*: 20 mg/g resin, 10 mg/mL bed volume Max. pressure 145 psi (10 bar)
* Binding capacity will vary for each polyhistidin-tagged protein
Applications • Purification of polyhistidine-tagged proteins • Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples • High protein concentration • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-IDA Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-IDA Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin
Technology: IMAC, immobilized metal ion affinity chromatography Chelating ligand: IDA (iminodiacetic acid) Format: Bulk matrix Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Binding capacity*: 20 mg/g resin, 10 mg/mL bed volume Max. pressure 145 psi (10 bar)
* Binding capacity will vary for each polyhistidin-tagged protein
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 150 Packed Columns, buffers 10 preps for the purification of His-tag proteins - Protino Ni-IDA 150 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 150 Packed Columns, buffers 10 preps for the purification of His-tag proteins - Protino Ni-TED 150 Packed Columns, buffers buffers
预装Ni-IDA的96孔预装板，高通量纯化His-tag蛋白。 • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield/recovery even from diluted samples • High protein concentration • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin ® storage at room temperature • Buffers included
Technology: IMAC (immobilized metal ion affinity chromatography) Chelating group: IDA (iminodiacetic acid) Format: ready-to-use 96-well plates Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Amount of resin per well: 50 mg Bed volume: 100 µL Binding capacity*: 1 mg/well (* refers to 6xHis-GFPuv)
Applications • Purification of polyhistidine-tagged proteins • 96-well format gravity flow chromatography Protino® 96 Ni-IDA Plates, buffers 1 x 96 preps for the purification of His-tag proteins - Protino Ni-IDA Plate, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 150 Packed Columns, buffers 50 preps for the purification of His-tag proteins - Protino Ni-TED 150 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 150 Packed Columns, buffers 50 preps for the purification of His-tag proteins - Protino Ni-IDA 150 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 1000 Packed Columns, buffers 50 preps for the purification of His-tag Proteins - Protino Ni-IDA 1000 Packed Columns, buffers
Ni-IDA预装纯化柱，提供缓冲液。IDA螯合基团，3个蛋白结合位点提供更高结合载量 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-IDA 2000 Packed Columns, buffers 25 preps for the purification of His-tag proteins - Protino Ni-IDA 2000 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 1000 Packed Columns, buffers 50 preps for the purification of His-tag proteins - Protino Ni-TED 1000 Packed Columns, buffers
Ni-TED预装纯化柱，提供缓冲液。Ted螯合基团，单个蛋白结合位点实现更高纯度和选择性 • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin • Buffers included • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices Protino® Ni-TED 2000 Packed Columns, buffers 25 preps for the purification of His-tag proteins - Protino Ni-TED 2000 Packed Columns, buffers
Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples • High protein concentration • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-IDA Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-IDA Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin
Technology: IMAC, immobilized metal ion affinity chromatography Chelating ligand: IDA (iminodiacetic acid) Format: Bulk matrix Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Binding capacity*: 20 mg/g resin, 10 mg/mL bed volume Max. pressure 145 psi (10 bar)
* Binding capacity will vary for each polyhistidin-tagged protein
Applications • Purification of polyhistidine-tagged proteins • Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield / recovery even from diluted samples • High protein concentration • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-IDA Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-IDA Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin
Technology: IMAC, immobilized metal ion affinity chromatography Chelating ligand: IDA (iminodiacetic acid) Format: Bulk matrix Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Binding capacity*: 20 mg/g resin, 10 mg/mL bed volume Max. pressure 145 psi (10 bar)
* Binding capacity will vary for each polyhistidin-tagged protein
Ni-TED纯化填料螯合Ted基团，可耐受高压高流速，可用于FPLC，高亲和高选择性得到更纯的His-tag蛋白，在还原和螯合试剂中亦能保持高度稳定，极低金属离子解离。干粉形式提供更易保存。有配套的空纯化柱和96孔纯化板提供，灵活多用。 High specificity for Purification of His-tag proteins, the purest alternative for His-tag protein purification • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin → storage at room temperature
Protino® Ni-TED Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-TED Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin
性价比的His-tag蛋白亲和纯化填料。性能出众价格低廉 The cost-effective NTA products for His-tag protein purification. Leading performance, unbeatable price • Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies) • High capacity and high affinity • Simply replace your current Ni-NTA products, no optimization or protocol change necessary • Purification under native and denaturing conditions • Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use • Market-leading performance for an unbeatable price
Protino® Ni-NTA Agarose • Aqueous suspension • For batch binding, gravity flow, and FPLC™ → highest flexibility of applications • Empty Protino® Columns for gravity flow available
Technology: IMAC (immobilized metal ion affinity chromatography) Chelating ligand NTA (nitrilotriacetic acid) Format: 50 % (v/v) aqueous suspension containing 30 % ethanol Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+ Bead size: 45–165 µm Binding capacity* ≤ 50 mg/mL Storage temperature: 4–8 °C
* Binding capacity will vary for each polyhistidine-tagged protein
Protino Ni-NTA suspension for the purification of His-tag proteins pack of 25 mL settled beaded agarose
预装FPLC柱。预装Ni-NTA Agarose，用于纯化His-tag蛋白。 Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose Applications • Purification of polyhistidine-tagged proteins • Compatible with automated liquid chromatography systems (MPLC, FPLC™, ÄKTAdesign™, etc.) as above FPLC columns (5 mL) for the purification of His-tag proteins pack of 5
预装Ni-IDA的96孔预装板，高通量纯化His-tag蛋白。 • Chelating group IDA (iminodiacetic acid), three protein binding sites • High protein yield/recovery even from diluted samples • High protein concentration • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices • Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin ® storage at room temperature • Buffers included
Technology: IMAC (immobilized metal ion affinity chromatography) Chelating group: IDA (iminodiacetic acid) Format: ready-to-use 96-well plates Matrix: Macroporous silica Physical form: Dry matrix, precharged with Ni2+ Amount of resin per well: 50 mg Bed volume: 100 µL Binding capacity*: 1 mg/well (* refers to 6xHis-GFPuv)
Applications • Purification of polyhistidine-tagged proteins • 96-well format gravity flow chromatography Protino® 96 Ni-IDA Plates, buffers 4 x 96 preps for the purification of His-tag proteins - Protino Ni-IDA Plates, buffers
