His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-TED, the purest alternative for His-tag protein purification.
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies);
• High stability against reducing / chelating agents;
• Low metal leaching;
• High protein purity;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature;
Protino® Ni-TED Packed Columns.
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin;
• Buffers included;
His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies);
• High protein yield / recovery even from diluted samples;
• High protein concentration;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature .
Protino® Ni-IDA Packed Column.
• Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin;
• Buffers included.
His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-TED, the purest alternative for His-tag protein purification.
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies);
• High stability against reducing / chelating agents;
• Low metal leaching;
• High protein purity;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature;
Protino® Ni-TED Packed Columns.
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin;
• Buffers included;
His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies);
• High protein yield / recovery even from diluted samples;
• High protein concentration;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature .
Protino® Ni-IDA Packed Column.
• Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin;
• Buffers included.
Protino Ni-TED镍柱专用树脂，
Protino® Ni-TED, the pure alternative for His-tag protein purification.
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies).
• High stability against reducing / chelating agents;
• Low metal leaching;
• High protein purity;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature ;
Protino® Ni-TED Resin
• Dry bulk material;
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility;
• Batch procedure, gravity-flow column chromatography, FPLC™ applications;
• Empty Protino® columns available for use with Protino® Ni-TED Resin;
• Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin.
Protino Ni-IVD镍柱专用树脂，
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites;
• High protein yield / recovery even from diluted samples;
• High protein concentration;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature.
Protino® Ni-IDA Resin;
• Dry bulk material;
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility;
• Batch procedure, gravity-flow column chromatography, FPLC™ applications;
• Empty Protino® columns available for use with Protino® Ni-IDA Resin;
• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin.
His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies);
• High protein yield / recovery even from diluted samples;
• High protein concentration;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature .
Protino® Ni-IDA Packed Column.
• Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin;
• Buffers included.
His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-TED, the purest alternative for His-tag protein purification.
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies);
• High stability against reducing / chelating agents;
• Low metal leaching;
• High protein purity;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature;
Protino® Ni-TED Packed Columns.
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin;
• Buffers included;
Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。
Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification;
• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies);
• High capacity and high affinity;
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary;
• Purification under native and denaturing conditions;
• Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use;
• Market-leading performance for an unbeatable price ;
Protino® Ni-NTA Columns;
• Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.
Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。
Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification;
• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies);
• High capacity and high affinity;
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary;
• Purification under native and denaturing conditions;
• Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use;
• Market-leading performance for an unbeatable price ;
Protino® Ni-NTA Columns;
• Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.
Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。
Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification;
• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies);
• High capacity and high affinity;
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary;
• Purification under native and denaturing conditions;
• Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use;
• Market-leading performance for an unbeatable price ;
Protino® Ni-NTA Columns;
• Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.
Protino Ni-NTA镍柱专用树脂，可用于大量样本的同时操作，独立分管，可避免交叉污染。
High-throughput purification of His-tag proteins
• Efficient protein purification using chelating group NTA (nitrilotriacetic acid)
• Unique Protino® Purification Plate for leak-free incubation during all steps
• Plate outlet and MN Wash Plate prevent cross-contamination
• High well-to-well reproducibility
• High capacity and high affinity
• Purification under native and denaturing conditions
• Processing under vacuum or by centrifugation
Protino Ni-IVD镍柱专用树脂，可用于大量样本的同时操作，独立分管，可避免交叉污染。
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites.
• High protein yield/recovery even from diluted samples.
• High protein concentration.
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices.
• Dry resin – storage at room temperature.
• Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin.
• Buffers included.
His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-TED, the purest alternative for His-tag protein purification.
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies);
• High stability against reducing / chelating agents;
• Low metal leaching;
• High protein purity;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature;
Protino® Ni-TED Packed Columns.
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin;
• Buffers included;
His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies);
• High protein yield / recovery even from diluted samples;
• High protein concentration;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature .
Protino® Ni-IDA Packed Column.
• Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin;
• Buffers included.
His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6×His-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators (10 µM). The binding of His-tags is stoichiometric and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA).
biotechrabbit™ Tris-NTA is available with a free amine group or conjugated to biotin. It can be used in a large range of applications, including protein detection and labeling, coupling proteins, lipids or cells to surfaces, protein purification and reversible protein modification.
His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies);
• High protein yield / recovery even from diluted samples;
• High protein concentration;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature .
Protino® Ni-IDA Packed Column.
• Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin;
• Buffers included.
His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies);
• High protein yield / recovery even from diluted samples;
• High protein concentration;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature .
Protino® Ni-IDA Packed Column.
• Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin;
• Buffers included.
His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-TED, the purest alternative for His-tag protein purification.
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies);
• High stability against reducing / chelating agents;
• Low metal leaching;
• High protein purity;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature;
Protino® Ni-TED Packed Columns.
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin;
• Buffers included;
His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。
Protino® Ni-TED, the purest alternative for His-tag protein purification.
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies);
• High stability against reducing / chelating agents;
• Low metal leaching;
• High protein purity;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature;
Protino® Ni-TED Packed Columns.
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin;
• Buffers included;
Protino Ni-TED镍柱专用树脂，
Protino® Ni-TED, the pure alternative for His-tag protein purification.
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies).
• High stability against reducing / chelating agents;
• Low metal leaching;
• High protein purity;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature ;
Protino® Ni-TED Resin
• Dry bulk material;
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility;
• Batch procedure, gravity-flow column chromatography, FPLC™ applications;
• Empty Protino® columns available for use with Protino® Ni-TED Resin;
• Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin.
Protino Ni-IVD镍柱专用树脂，
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites;
• High protein yield / recovery even from diluted samples;
• High protein concentration;
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices;
• Dry resin – storage at room temperature.
Protino® Ni-IDA Resin;
• Dry bulk material;
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility;
• Batch procedure, gravity-flow column chromatography, FPLC™ applications;
• Empty Protino® columns available for use with Protino® Ni-IDA Resin;
• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin.
His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6×His-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators (10 µM). The binding of His-tags is stoichiometric and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA).
biotechrabbit™ Tris-NTA is available with a free amine group or conjugated to biotin. It can be used in a large range of applications, including protein detection and labeling, coupling proteins, lipids or cells to surfaces, protein purification and reversible protein modification.
Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。
Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification;
• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies);
• High capacity and high affinity;
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary;
• Purification under native and denaturing conditions;
• Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use;
• Market-leading performance for an unbeatable price ;
Protino® Ni-NTA Columns;
• Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.
Protino Ni-IVD镍柱专用树脂，可用于大量样本的同时操作，独立分管，可避免交叉污染。
Protino® Ni-IDA, best choice for higher yields of His-tag proteins.
• Chelating group IDA (iminodiacetic acid), three protein binding sites.
• High protein yield/recovery even from diluted samples.
• High protein concentration.
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices.
• Dry resin – storage at room temperature.
• Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin.
• Buffers included.
Protino Ni-NTA镍柱专用树脂，可用于大量样本的同时操作，独立分管，可避免交叉污染。
High-throughput purification of His-tag proteins
• Efficient protein purification using chelating group NTA (nitrilotriacetic acid)
• Unique Protino® Purification Plate for leak-free incubation during all steps
• Plate outlet and MN Wash Plate prevent cross-contamination
• High well-to-well reproducibility
• High capacity and high affinity
• Purification under native and denaturing conditions
• Processing under vacuum or by centrifugation
