Protino® Ni-NTA 琼脂糖，用于His-Tag 蛋白纯化 Protino® Ni-NTA Agarose; • Aqueous suspension; • For batch binding, gravity flow, and FPLC™ – highest flexibility of applications; • Empty Protino® Columns for gravity flow available.

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

Protino Ni-TED镍柱专用树脂， Protino® Ni-TED, the pure alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies). • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature ; Protino® Ni-TED Resin • Dry bulk material; • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility; • Batch procedure, gravity-flow column chromatography, FPLC™ applications; • Empty Protino® columns available for use with Protino® Ni-TED Resin; • Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin.

Protino Ni-IVD镍柱专用树脂， Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites; • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature. Protino® Ni-IDA Resin; • Dry bulk material; • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility; • Batch procedure, gravity-flow column chromatography, FPLC™ applications; • Empty Protino® columns available for use with Protino® Ni-IDA Resin; • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin.

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。 Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification; • Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies); • High capacity and high affinity; • Simply replace your current Ni-NTA products, no optimization or protocol change necessary; • Purification under native and denaturing conditions; • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use; • Market-leading performance for an unbeatable price ; Protino® Ni-NTA Columns; • Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.

Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。 Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification; • Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies); • High capacity and high affinity; • Simply replace your current Ni-NTA products, no optimization or protocol change necessary; • Purification under native and denaturing conditions; • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use; • Market-leading performance for an unbeatable price ; Protino® Ni-NTA Columns; • Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.

Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。 Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification; • Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies); • High capacity and high affinity; • Simply replace your current Ni-NTA products, no optimization or protocol change necessary; • Purification under native and denaturing conditions; • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use; • Market-leading performance for an unbeatable price ; Protino® Ni-NTA Columns; • Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.

Protino Ni-NTA镍柱专用树脂，可用于大量样本的同时操作，独立分管，可避免交叉污染。 High-throughput purification of His-tag proteins    • Efficient protein purification using chelating group NTA (nitrilotriacetic acid) • Unique Protino® Purification Plate for leak-free incubation during all steps • Plate outlet and MN Wash Plate prevent cross-contamination • High well-to-well reproducibility • High capacity and high affinity • Purification under native and denaturing conditions • Processing under vacuum or by centrifugation

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

Protino Ni-IVD镍柱专用树脂，可用于大量样本的同时操作，独立分管，可避免交叉污染。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites. • High protein yield/recovery even from diluted samples. • High protein concentration. • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices. • Dry resin – storage at room temperature. • Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin. • Buffers included.

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

His-Tag 镍柱，His标签蛋白纯化的更佳选择，已TED作为螯合剂制备树脂，低金属浸出，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-TED, the purest alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies); • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature; Protino® Ni-TED Packed Columns. • Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin; • Buffers included;

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

His-Tag 镍柱，以IDA作为螯合剂制备树脂,His标签蛋白纯化的高得率更佳选择，低非特异结合，蛋白纯度高。包含镍柱，树脂，缓冲液。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies); • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature . Protino® Ni-IDA Packed Column. • Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin; • Buffers included.

His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6×His-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators (10 µM). The binding of His-tags is stoichiometric and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA). biotechrabbit™ Tris-NTA is available with a free amine group or conjugated to biotin. It can be used in a large range of applications, including protein detection and labeling, coupling proteins, lipids or cells to surfaces, protein purification and reversible protein modification.

Protino Ni-TED镍柱专用树脂， Protino® Ni-TED, the pure alternative for His-tag protein purification. • Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding site provides highest selectivity and purity (also see Technologies). • High stability against reducing / chelating agents; • Low metal leaching; • High protein purity; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature ; Protino® Ni-TED Resin • Dry bulk material; • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility; • Batch procedure, gravity-flow column chromatography, FPLC™ applications; • Empty Protino® columns available for use with Protino® Ni-TED Resin; • Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin.

Protino Ni-IVD镍柱专用树脂， Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites; • High protein yield / recovery even from diluted samples; • High protein concentration; • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices; • Dry resin – storage at room temperature. Protino® Ni-IDA Resin; • Dry bulk material; • Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell – highest flexibility; • Batch procedure, gravity-flow column chromatography, FPLC™ applications; • Empty Protino® columns available for use with Protino® Ni-IDA Resin; • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin.

Protino® Ni-NTA 琼脂糖，用于His-Tag 蛋白纯化 Protino® Ni-NTA Agarose; • Aqueous suspension; • For batch binding, gravity flow, and FPLC™ – highest flexibility of applications; • Empty Protino® Columns for gravity flow available.

His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit Tris-NTA complexes three NTA groups that together bind a 6×His-tag with an affinity that is four orders of magnitude higher (1 nM) than is possible with conventional chelators (10 µM). The binding of His-tags is stoichiometric and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA). biotechrabbit™ Tris-NTA is available with a free amine group or conjugated to biotin. It can be used in a large range of applications, including protein detection and labeling, coupling proteins, lipids or cells to surfaces, protein purification and reversible protein modification.

Protino Ni-NTA 镍柱, 已填充Protino® Ni-NTA 琼脂糖。 Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification; • Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies); • High capacity and high affinity; • Simply replace your current Ni-NTA products, no optimization or protocol change necessary; • Purification under native and denaturing conditions; • Suitable for small proteins, large protein complexes, proteins with low expression rates – universal use; • Market-leading performance for an unbeatable price ; Protino® Ni-NTA Columns; • Ready-to-use FPLC™ columns filled with Protino® Ni-NTA Agarose.

Protino Ni-IVD镍柱专用树脂，可用于大量样本的同时操作，独立分管，可避免交叉污染。 Protino® Ni-IDA, best choice for higher yields of His-tag proteins. • Chelating group IDA (iminodiacetic acid), three protein binding sites. • High protein yield/recovery even from diluted samples. • High protein concentration. • High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices. • Dry resin – storage at room temperature. • Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin. • Buffers included.

Protino Ni-NTA镍柱专用树脂，可用于大量样本的同时操作，独立分管，可避免交叉污染。 High-throughput purification of His-tag proteins    • Efficient protein purification using chelating group NTA (nitrilotriacetic acid) • Unique Protino® Purification Plate for leak-free incubation during all steps • Plate outlet and MN Wash Plate prevent cross-contamination • High well-to-well reproducibility • High capacity and high affinity • Purification under native and denaturing conditions • Processing under vacuum or by centrifugation