Ready-to-Package Catalog Plasmid Library Agilent’s catalog CRISPR guide libraries target all exonic sequences in the human and mouse genome and are ideal for genome-wide functional screening. These libraries contain content developed by and licensed from the Broad Institute at MIT. In comparison to other GeCKO libraries, Agilent’s GeCKO libraries contain fewer missed guides and more uniform representation across the library. Both the mouse and human GeCKO libraries come as plasmids, with a puromycin selection marker and a GFP reporter, that can be used to generate high-titers of lentiviral particles. Benefits Exome-wide knock-down screening - Validated guides targeting all known genes Highest quality, sequence-verified GeCKOv2 libraries Lentiviral, ready-to-package plasmid libraries Human and mouse libraries - Common model organisms/cell lines for genome-wide screening Full flexibility to develop your research applications For Research Use Only. Not for use in diagnostic procedures.

Ready-to-Package Catalog Plasmid Library Agilent’s catalog CRISPR guide libraries target all exonic sequences in the human and mouse genome and are ideal for genome-wide functional screening. These libraries contain content developed by and licensed from the Broad Institute at MIT. In comparison to other GeCKO libraries, Agilent’s GeCKO libraries contain fewer missed guides and more uniform representation across the library. Both the mouse and human GeCKO libraries come as plasmids, with a puromycin selection marker and a GFP reporter, that can be used to generate high-titers of lentiviral particles. Benefits Exome-wide knock-down screening - Validated guides targeting all known genes Highest quality, sequence-verified GeCKOv2 libraries Lentiviral, ready-to-package plasmid libraries Human and mouse libraries - Common model organisms/cell lines for genome-wide screening Full flexibility to develop your research applications For Research Use Only. Not for use in diagnostic procedures.

The library cloning kit uses Agilent’s SureVector chemistry to allow the rapid generation of plasmid libraries from an amplified oligo pool. Included in the kit are Agilent’s linearized pSGLenti vector backbone, competent cells, media, and all buffers and reagents required to clone Agilent’s pooled ready-to-clone or ready-to-amplify libraries. pSGLenti is directly compatible with Agilent’s ready-to-clone libraries while alternative backbones, supplied separately, can also be used to clone fully custom, ready-to-amplify libraries. Each kit contains sufficient amounts of the above materials to generate four plasmid libraries of 60,000 guides. For Research Use Only. Not for Use in Diagnostic Procedures.