PNPP 是一类磷酸酶非特异性底物，非蛋白质成分，常用语对蛋白碱性和酸性磷酸酶进行分析；常借助于对磷酸酶催化其水解的能力对酶进行单点或者连续的分光光度测量分析。p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases. The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol, a chromogenic product with absorbance at 405 nm. The assay can be used for the quick analysis of the protein phosphatase activity under any non-standard conditions.
PNPP 是一类磷酸酶非特异性底物，非蛋白质成分，常用语对蛋白碱性和酸性磷酸酶进行分析；常借助于对磷酸酶催化其水解的能力对酶进行单点或者连续的分光光度测量分析。p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases. The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol, a chromogenic product with absorbance at 405 nm. The assay can be used for the quick analysis of the protein phosphatase activity under any non-standard conditions.
