此抗体套装包含Atg12 Antibody (Human Specific) #2010、LC3B (D11) XP® Rabbit mAb #3868、LC3A (D50G8) XP® Rabbit mAb #4599各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Autophagosome Marker Antibody Sampler 试剂盒提供了一种经济有效的方法，能够检测细胞中积累的自噬体。这种试剂盒含有足够的一抗和二抗，每个一抗可以进行四次western blots。Autophagosome Marker Antibody Sampler 试剂盒中的每个抗体可以检测到内源性的目标总蛋白。Atg12抗体（人源特异性）能够识别游离的Atg12或Atg12-Atg15复合物。LC3A (D50G8) XP® Rabbit mAb 兔单抗和 LC3B (D11) XP® Rabbit mAb 兔单抗能够与其它LC3异构体发生交叉反应，并且对于II型LC3的反应要更强。The Autophagosome Marker Antibody Sampler Kit provides an economical means to investigate the accumulation of autophagosomes within the cell. The kit contains enough primary and secondary antibodies to perform four western blots per primary antibody.Each antibody in the Autophagosome Marker Antibody Sampler Kit detects endogenous levels of total respective protein. Atg12 Antibody (Human Specific) recognizes free Atg12 and the Atg12-Atg5 conjugate. LC3A (D50G8) XP® Rabbit mAb and LC3B (D11) XP® Rabbit mAb may cross-react with other LC3 isoforms and have stronger reactivity with the type II form.

此kit包含Oct-4A (C30A3) Rabbit mAb #2840、Sox2 (D6D9) XP® Rabbit mAb #3579、Nanog (D73G4) XP® Rabbit mAb #4903各20µl。StemLight多潜能性转录因子抗体试剂盒包括一系列能够检测ES和iPS细胞中主要转录网络中关键组分蛋白包括Oct-4, Nanog, 和Sox2的抗体。本试剂盒可用于示踪人胚胎干细胞（ES）和诱导的多潜能干细胞（iPS）的多潜能。失去这些标志性蛋白意味着多潜能性的丧失或细胞分化。试剂盒中组分已经优化为标准稀释度用于在免疫荧光分析中配套使用，但是这些组件也能用于其他实验——请参阅单独的使用说明以寻找具体建议。试剂足够进行160次反应体系为100ul的免疫荧光实验。Nanog (D73G4) XP® Rabbit mAb能够检测内源性的总Nanog蛋白。Oct-4A (C30A3) Rabbit mAb能够识别内源性的总Oct-4A蛋白。Sox2 (D6D9) XP® Rabbit mAb能够识别内源性的总Sox2蛋白。StemLight™ Pluripotency Transcription Factor Antibody Kit contains a panel of antibodies for the detection of Oct-4, Nanog, and Sox2, key components of the core pluripotency transcription network in embryonic stem (ES) and induced pluripotent stem (iPS) cells. The kit can be used to track the pluripotent potential of human ES or iPS cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis at a standard dilution, but components are also validated for use in other applications - please refer to individual datasheet information for application specific recommendations. Enough reagents are provided for 160 immunofluorescent assays based on a working volume of 100 μl.Nanog (D73G4) XP® Rabbit mAb detects endogenous levels of total Nanog protein. Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. Sox2 (D6D9) XP® Rabbit mAb detects endogenous levels of total Sox2 protein.

此kit包含TRA-1-81 (TRA-1-81) Mouse mAb #4745、TRA-1-60(S) (TRA-1-60(S)) Mouse mAb #4746、SSEA4 (MC813) Mouse mAb #4755 各20µl。StemLight™表面标记试剂盒包括一系列抗体，能够识别特异性表达于人多潜能干细胞表面的抗原。本试剂盒可用于示踪人胚胎干细胞（ES）和诱导的多潜能干细胞（iPS）的多潜能。失去这些标志性蛋白意味着多潜能性的丧失或细胞分化。本试剂盒所包含的成分已经提前优化使其也适用于免疫荧光分析。TRA-1-60(S) (TRA-1-60(S)) Mouse mAb鼠单抗可识别内源性神经氨酸酶敏感的TRA-1-60抗原蛋白。TRA-1-81 (TRA-1-81) Mouse mAb鼠单抗识别内源性的TRA-1-81抗原蛋白。SSEA4 (MC813) Mouse mAb鼠单抗识别内源性SSEA4抗原蛋白。The StemLight™ Surface Marker Kit contains a panel of antibodies for the detection of antigens that are specifically expressed on the surface of human pluripotent cells. The kit can be used to track the pluripotent potential of human embryonic stem (ES) or induced pluripotent (iPS) cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis.TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen. TRA-1-81 (TRA-1-81) Mouse mAb detects endogenous levels of TRA-1-81 antigen. SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen.

此抗体套装包含MAPKAPK-2 (D1E11) Rabbit mAb #12155、Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007、Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。MAPKAPK-2 Antibody Sampler Kit为评估特定位点磷酸化的MAPKAPK-2蛋白水平，以及总MAPKAPK-2蛋白水平提供了一个经济的方法。该试剂盒所提供的一抗和二抗可以完成四次Western blot实验。MAPKAPK-2 Antibody Sampler Kit中每个抗体都能检测它所对应的内源性靶点蛋白水平。活化状态的抗体只能检测所列物种中的磷酸化靶蛋白。MAPKAPK-2 Antibody不能与MAPKAPK-3 、MAPKAPK-5发生交叉反应。The MAPKAPK-2 Antibody Sampler Kit provides an economical means of evaluating levels of MAPKAPK-2 protein phosphorylated at specific sites, as well as total MAPKAPK-2 protein levels. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.Each antibody in the MAPKAPK-2 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. MAPKAPK-2 Antibody does not cross-react with MAPKAPK-3 or -5.

此抗体套装包含GCN5L2 (C26A10) Rabbit mAb #3305、PCAF (C14G9) Rabbit mAb #3378、Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771、CBP (D6C5) Rabbit mAb #7389各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Lysine Acetyltransferase Antibody Sampler Kit可以提供一种快速经济的方法来检测内源性赖氨酸乙酰转移酶的水平，包括Acetyl-CBP, CBP, GCN5L2, 和 PCAF。试剂盒提供的每种一抗都足够进行4次western blot实验。Lysine Acetyltransferase Antibody Sampler Kit中的每种抗体能够识别各自的内源性的目的蛋白。该抗体不会与其他家族成员产生交叉反应。The Lysine Acetyltransferase Antibody Sampler Kit provides an economical means to examine several lysine acetyltrasferases, including: Acetyl-CBP, CBP, GCN5L2, and PCAF. The kit contains enough primary antibody to perform four western blots per primary.Each antibody in the Lysine Acetyltransferase Antibody Sampler Kit recognizes endogenous levels of respective target protein. The antibodies do not cross-react with other family members.

kit包括Histone H3 (D1H2) XP® Rabbit mAb 499T*1、AIF (D39D2) XP® Rabbit mAb 5318T*1、Vimentin (D21H3) XP® Rabbit mAb 5741T*1、Anti-rabbit IgG, HRP-linked Antibody 7074P2*1和 MEK1/2 (D1A5) Rabbit mAb 8727T*1 。Cell Fractionation Antibody Sampler Kit中的每种抗体都能识别内源性水平的它们各自的目标蛋白。抗体与其他家族成员没有交叉反应。每种抗体都用Cell Fractionation Kit #9038进行了验证。Cell Fractionation Antibody Sampler Kit提供了一种经济的方法来检测每个分离的亚细胞成分的纯度。 Each antibody in the Cell Fractionation Antibody Sampler Kit recognizes endogenous levels of its respective target protein. The antibodies do not cross-react with other family members.The Cell Fractionation Antibody Sampler Kit provides an economical means for determining the purity of each distinctly separated subcellular fraction by western blot using Cell Signaling Technology's Cell Fractionation Kit #9038.

此抗体组合kit包含2402T*1、2404T*1、2405T*1、7074P2*1、7076P2*1、9709T*1。The HSP27 Antibody Kit provides an economical means to evaluate the activation status of the HSP27 protein. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.Each antibody in the HSP27 Antibody Sampler Kit recognizes endogenous levels of its specific target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site.Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27 and to full-length human HSP27 protein. Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser15 or Ser78 of human HSP27 protein. Antibodies are purified by protein A and peptide affinity chromatography.

此抗体套装包含Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818T、Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676T、Acetyl-CoA Carboxylase 1 Antibody #4190T、Anti-rabbit IgG, HRP-linked Antibody #7074P2、Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb #8578T。Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler试剂盒提供了一种经济的方式以区分两种acetyl-CoA carboxylase亚型，以及total acetyl-CoA carboxylase 和 phosphorylated acetyl-CoA carboxylase。该试剂盒提供了足以进行4次western blot分析的一抗。每个抗体都可以识别其内源性的靶蛋白。Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗 可以识别Ser79被磷酸化的内源性acetyl-CoA carboxylase。合成对应human acetyl-CoA carboxylase 1蛋白序列的肽段免疫动物获得多抗。多抗经过蛋白A和肽段亲和层析纯化获得。合成磷酸肽段免疫动物获得单抗，磷酸肽段和human acetyl-CoA carboxylase蛋白的Ser79及其邻近氨基酸序列一致，或和human acetyl-CoA carboxylase α1蛋白的Ser523或human acetyl-CoA carboxylase 2的Val1416获得单抗，磷酸肽段和human acetyl-CoA carboxylase蛋白的Ser79。The Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit provides an economical means of distinguishing between the two acetyl-CoA carboxylase isoforms, and between total acetyl-CoA carboxylase and phosphorylated acetyl-CoA carboxylase. The kit includes enough antibody to perform four western blot experiments per primary antibody.Each antibody recognizes endogenous levels of their target protein. Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human acetyl-CoA carboxylase 1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein, or a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1, or Val1416 of human acetyl-CoA carboxylase 2 protein.

该抗体套装含Notch1 (D1E11) XP® Rabbit mAb #3608T、Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147T、Notch3 (D11B8) Rabbit mAb #5276T、Notch2 (D76A6) XP® Rabbit mAb #5732T和Anti-rabbit IgG, HRP-linked Antibody #7074P2各一。Notch Isoform Antibody Sampler Kit提供经济合适的方式检测Notch信号通路。试剂盒含有足够做四次小的Western blot所需的一抗和二抗。Cleaved Notch1 (Val1744) (D3B8) XP® Rabbit mAb兔单抗只识别1743位甘氨酸和1744位缬氨酸之间被剪切的内源性Notch1蛋白的胞质结构域。抗体不识别全长Notch1蛋白或者被其他位点的剪切型Notch1。在不同细胞中存在Notch1的远处羧基末端的突变，因此此抗体识别大小不同的剪切型Notch1（见参考文献6）Notch1 (D6F11) XP® Rabbit mAb兔源抗体检测内源性的总Notch1蛋白。它识别全长(~300 kDa)和跨膜/胞内区域NTM(~120 kDa)。Notch2 (D67C8) XPTM兔源单抗识别内源性的Notch2蛋白。它识别全长(~300 kDa)和跨膜/胞内区域NTM(~110 kDa)。Notch3 (D11B8) Rabbit mAb兔抗体识别内源性的总Notch3蛋白。此抗体识别全长270kDa的Notch3和截短的蛋白(NTM)，包含短的胞外区域，跨膜区域和胞内区域（90kDa）。The Notch Isoform Antibody Sampler Kit provides an economical means to investigate Notch Signaling. The kit contains primary and secondary antibodies to perform four western mini-blots with each antibody.Cleaved Notch1 (Val1744) (D3B8) XP® Rabbit mAb detects endogenous levels of the cytosolic domain of Notch1 only when cleaved between Gly1743 and Val1744. The antibody does not recognize full length Notch1 or Notch1 cleaved at other positions. It detects cleaved Notch1 with different sizes in different cell lines due to mutations at the distant C-termini of Notch1 (see ref 6). Notch1 (D6F11) XP® Rabbit detects endogenous level of total notch1 protein. It recognizes both the full-length (~300 kDa) and the transmembrane/intracellular region NTM (~120 kDa). Notch2 (D67C8) XPTM Rabbit mAb detects endogenous levels of total Notch2 protein. It recognizes both the full-length (~ 300 kDa) and the transmembrane/intracellular region NTM (~110 kDa). Notch3 (D11B8) Rabbit mAb detects endogenous levels of total Notch3 protein. The antibody recognizes both full-length (FL) Notch3 at 270 kDa and a truncated protein (NTM) containing a short extracellullar region, the transmembrane domain and the intracellular region at 90 kDa.

该抗体套装包含Aurora A (D3E4Q) Rabbit mAb #14475、Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914T、Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079T、Aurora B/AIM1 Antibody #3094T和Anti-rabbit IgG, HRP-linked Antibody #7074P2。Aurora Antibody Sampler Kit为研究细胞周期G2/M期提供了一个经济的方式。该试剂盒内含足够4次western blot实验的一抗和二抗。Aurora Antibody Sampler Kit中的每个抗体都能够检测其内源性靶蛋白，且不与家族其他成员发生交叉反应。The Aurora Antibody Sampler Kit provides an economical means to investigate the G2/M phase of the cell cycle. The kit contains enough primary and secondary antibodies to perform four Western blots with each antibody.Each antibody in the Aurora Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.

此抗体套装包含IRAK4 Antibody #4363、IRAK2 Antibody #4367、IRAK-M Antibody #4369、IRAK1 (D51G7) Rabbit mAb #4504各20µl，和Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl。IRAK Isoform Antibody Sampler Kit 中的每一个抗体都能检测其目标蛋白的内源水平蛋白。在内源水平上并未发现抗体与其它家族成员有交叉反应。IRAK Isoform Antibody Sampler Kit为研究4个IL-1受体相关激酶家族成员 IRAK1, IRAK2, IRAK3/IRAK-M 和 IRAK4的总蛋白水平提供了经济有效的方法。Each antibody in the IRAK Isoform Antibody Sampler Kit detects endogenous levels of its indicated target. Cross-reactivity has not been detected with other family members at endogenous levels.The IRAK Isoform Antibody Sampler Kit provides an economical means to examine total protein levels of the four Interleukin-1 Receptor Associated Kinase family members: IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4.

此抗体套装包含Presenilin 1 (D39D1) Rabbit mAb #5643、Nicastrin (D38F9) Rabbit mAb #5665、PEN2 (D6G8) Rabbit mAb #8598、Presenilin 2 (D30G3) Rabbit mAb #9979 各20µl，以及Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Presenilin 1 (D39D1) Rabbit mAb 兔单抗识别内源性的Presenilin 1 总蛋白，该抗体也可以识别非特异的60kDa条带。Presenilin 2 Antibody 兔多抗识别内源性的Presenilin 2的羧基末端片段（约23 kDa），稍弱的识别全长蛋白（54kDa）。它不与内源性的presenilin 1发生交叉反应。Nicastrin (D38F9) Rabbit mAb识别内源性的Nicastrin总蛋白。PEN2 Antibody 兔多抗识别内源性的PEN2总蛋白。γ Secretase Antibody Sampler Kit 小包装抗体试剂盒提供了一个经济的手段来研究γ-分泌酶复合体。该试剂盒包含了足够的一抗和二抗以满足每款抗体用于4张膜的免疫印迹实验。resenilin 1 (D39D1) Rabbit mAb recognizes endogenous levels of total Presenilin 1 protein. The antibody may detect a nonspecific band at 60 kDa. Presenilin 2 Antibody detects endogenous levels of the carboxy-terminal fragment of Presenilin 2 (23 kDa) and the full length protein (54 kDa) to a lesser extent. It does not cross-react with endogenous levels of presenilin 1. Nicastrin (D38F9) Rabbit mAb recognizes endogenous levels of total Nicastrin protein. PEN2 Antibody detects endogenous levels of total PEN2 protein.The γ Secretase Antibody Sampler Kit provides an economical means of evaluating components of the gamma secretase complex. The kit contains enough primary and secondary antibodies to perform four western miniblot experiments.

此抗体套装包含Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb #13944、Acetyl-Histone H4 (Lys8) Antibody #2594、Histone H4 (L64C1) Mouse mAb #2935、Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb #8647各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 和Anti-mouse IgG, HRP-linked Antibody #7076各100µl。Acetyl-Histone H4 Antibody Sampler Kit提供了一个经济的方法去检测histone H4总蛋白和在Lys12、Lys5和Lys8位点乙酰化的histone H4蛋白。该试剂盒包含的一抗和二抗可以进行四次免疫印迹实验。每一个抗体仅可检测特异性靶蛋白，并且不能与其它家族成员发生交叉反应。The Acetyl-Histone H4 Antibody Sampler Kit provides an economical means of detecting total histone H4 as well as histone H4 acetylated at various residues including Lys12, Lys5, and Lys8. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.Each antibody in this kit recognizes only its specific target protein and does not cross-react with other family members.

此抗体套装包含TRAF1 (45D3) Rabbit mAb #4715、TRAF2 (C192) Antibody #4724、TRAF3 Antibody #4729、TRAF6 (D21G3) Rabbit mAb #8028各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。TRAF Antibody Sampler Kit 提供了一种经济有效的方法评测内源性水平的TRAF1，2，3和6。试剂盒包括足够的一级和二级抗体来进行4个迷你免疫印迹实验。TRAF1 (45D3) Rabbit mAb能够检测内源性水平TRAF1总蛋白。TRAF2 (C192) Antibody 能够检测内源性水平人TRAF2总蛋白。TRAF3 Antibody能够检测内源性水平TRAF3总蛋白。TRAF6 (D21G3) Rabbit mAb能够检测内源性水平TRAF6总蛋白。未检测到生理条件下其它家族成员的交叉反应。The TRAF Antibody Sampler Kit provides an economical means to evaluate endogenous levels of TRAF1, 2, 3, and 6. The kit contains enough primary and secondary antibodies to perform four western mini-blot experiments.TRAF1 (45D3) Rabbit mAb detects endogenous levels of total TRAF1 protein. TRAF2 (C192) Antibody detects endogenous levels of total human TRAF2 protein. TRAF3 Antibody detects endogenous levels of total TRAF3 protein. TRAF6 (D21G3) Rabbit mAb detects endogenous levels of total TRAF6 protein. Cross-reactivity was not detected with other family members at physiological conditions.

此抗体套装包含Phospho-Tuberin/TSC2 (Ser939) Antibody #3615、Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb #3617、Tuberin/TSC2 (D93F12) XP® Rabbit mAb #4308、Phospho-Tuberin/TSC2 (Ser1387) Antibody #5584各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。The Phospho-TSC2 Antibody Sampler Kit 为研究细胞内蛋白的折叠提供了经济的手段。本试剂盒包含足够的一抗和二抗来进行4次western blot实验。Phospho-TSC2 Antibody Sampler Kit中的每一个抗体可以识别其特异的靶目标，不会与其他家族成员发生交叉反应，除非已经说明。Phospho-Tuberin/TSC2 (Ser1387) Antibody可以检测Ser1387磷酸化的内源性Tuberin水平。本抗体可以与一个未知的140KD的蛋白发生交叉反应。The Phospho-TSC2 Antibody Sampler Kit provides an economical means to investigate protein folding within the cell. The kit contains enough primary and secondary antibodies to perform four western blot experiments per primary antibody.Each antibody in the Phospho-TSC2 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members unless otherwise indicated. Phospho-Tuberin/TSC2 (Ser1387) Antibody detects endogenous levels of tuberin protein only when phosphorylated at Ser1387. This antibody also cross-reacts with an unidentified 140 kDa protein.