此抗体套装包含Atg12 Antibody (Human Specific) #2010、LC3B (D11) XP® Rabbit mAb #3868、LC3A (D50G8) XP® Rabbit mAb #4599各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Autophagosome Marker Antibody Sampler 试剂盒提供了一种经济有效的方法，能够检测细胞中积累的自噬体。这种试剂盒含有足够的一抗和二抗，每个一抗可以进行四次western blots。Autophagosome Marker Antibody Sampler 试剂盒中的每个抗体可以检测到内源性的目标总蛋白。Atg12抗体（人源特异性）能够识别游离的Atg12或Atg12-Atg15复合物。LC3A (D50G8) XP® Rabbit mAb 兔单抗和 LC3B (D11) XP® Rabbit mAb 兔单抗能够与其它LC3异构体发生交叉反应，并且对于II型LC3的反应要更强。The Autophagosome Marker Antibody Sampler Kit provides an economical means to investigate the accumulation of autophagosomes within the cell. The kit contains enough primary and secondary antibodies to perform four western blots per primary antibody.Each antibody in the Autophagosome Marker Antibody Sampler Kit detects endogenous levels of total respective protein. Atg12 Antibody (Human Specific) recognizes free Atg12 and the Atg12-Atg5 conjugate. LC3A (D50G8) XP® Rabbit mAb and LC3B (D11) XP® Rabbit mAb may cross-react with other LC3 isoforms and have stronger reactivity with the type II form.

标记：Purified

此kit包含Oct-4A (C30A3) Rabbit mAb #2840、Sox2 (D6D9) XP® Rabbit mAb #3579、Nanog (D73G4) XP® Rabbit mAb #4903各20µl。StemLight多潜能性转录因子抗体试剂盒包括一系列能够检测ES和iPS细胞中主要转录网络中关键组分蛋白包括Oct-4, Nanog, 和Sox2的抗体。本试剂盒可用于示踪人胚胎干细胞（ES）和诱导的多潜能干细胞（iPS）的多潜能。失去这些标志性蛋白意味着多潜能性的丧失或细胞分化。试剂盒中组分已经优化为标准稀释度用于在免疫荧光分析中配套使用，但是这些组件也能用于其他实验——请参阅单独的使用说明以寻找具体建议。试剂足够进行160次反应体系为100ul的免疫荧光实验。Nanog (D73G4) XP® Rabbit mAb能够检测内源性的总Nanog蛋白。Oct-4A (C30A3) Rabbit mAb能够识别内源性的总Oct-4A蛋白。Sox2 (D6D9) XP® Rabbit mAb能够识别内源性的总Sox2蛋白。StemLight™ Pluripotency Transcription Factor Antibody Kit contains a panel of antibodies for the detection of Oct-4, Nanog, and Sox2, key components of the core pluripotency transcription network in embryonic stem (ES) and induced pluripotent stem (iPS) cells. The kit can be used to track the pluripotent potential of human ES or iPS cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis at a standard dilution, but components are also validated for use in other applications - please refer to individual datasheet information for application specific recommendations. Enough reagents are provided for 160 immunofluorescent assays based on a working volume of 100 μl.Nanog (D73G4) XP® Rabbit mAb detects endogenous levels of total Nanog protein. Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. Sox2 (D6D9) XP® Rabbit mAb detects endogenous levels of total Sox2 protein.

标记：Purified

此kit包含TRA-1-81 (TRA-1-81) Mouse mAb #4745、TRA-1-60(S) (TRA-1-60(S)) Mouse mAb #4746、SSEA4 (MC813) Mouse mAb #4755 各20µl。StemLight™表面标记试剂盒包括一系列抗体，能够识别特异性表达于人多潜能干细胞表面的抗原。本试剂盒可用于示踪人胚胎干细胞（ES）和诱导的多潜能干细胞（iPS）的多潜能。失去这些标志性蛋白意味着多潜能性的丧失或细胞分化。本试剂盒所包含的成分已经提前优化使其也适用于免疫荧光分析。TRA-1-60(S) (TRA-1-60(S)) Mouse mAb鼠单抗可识别内源性神经氨酸酶敏感的TRA-1-60抗原蛋白。TRA-1-81 (TRA-1-81) Mouse mAb鼠单抗识别内源性的TRA-1-81抗原蛋白。SSEA4 (MC813) Mouse mAb鼠单抗识别内源性SSEA4抗原蛋白。The StemLight™ Surface Marker Kit contains a panel of antibodies for the detection of antigens that are specifically expressed on the surface of human pluripotent cells. The kit can be used to track the pluripotent potential of human embryonic stem (ES) or induced pluripotent (iPS) cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis.TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen. TRA-1-81 (TRA-1-81) Mouse mAb detects endogenous levels of TRA-1-81 antigen. SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen.

此抗体套装包含MAPKAPK-2 (D1E11) Rabbit mAb #12155、Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007、Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。MAPKAPK-2 Antibody Sampler Kit为评估特定位点磷酸化的MAPKAPK-2蛋白水平，以及总MAPKAPK-2蛋白水平提供了一个经济的方法。该试剂盒所提供的一抗和二抗可以完成四次Western blot实验。MAPKAPK-2 Antibody Sampler Kit中每个抗体都能检测它所对应的内源性靶点蛋白水平。活化状态的抗体只能检测所列物种中的磷酸化靶蛋白。MAPKAPK-2 Antibody不能与MAPKAPK-3 、MAPKAPK-5发生交叉反应。The MAPKAPK-2 Antibody Sampler Kit provides an economical means of evaluating levels of MAPKAPK-2 protein phosphorylated at specific sites, as well as total MAPKAPK-2 protein levels. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.Each antibody in the MAPKAPK-2 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. MAPKAPK-2 Antibody does not cross-react with MAPKAPK-3 or -5.

此抗体套装包含Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb #3073、c-Kit (D13A2) XP® Rabbit mAb #3074、Phospho-c-Kit (Tyr719) Antibody #3391各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。c-Kit Antibody Sampler Kit是检测特异位点磷酸化的c-Kit蛋白和总c-Kit蛋白水平的快速、经济的选择。本试剂盒的一抗和二抗足够四次免疫印记检测实验使用。磷酸化c-Kit的抗体在c-Kit的特殊位点被磷酸化时能够检测到内源c-Kit的表达。抗c-Kit的兔源单克隆抗体 (D13A2) XP® 能检测内源总c-kit蛋白的水平。The c-Kit Antibody Sampler Kit provides a fast and economical means of evaluating levels of c-Kit receptor protein phosphorylated at the specified sites, as well as total c-Kit receptor levels. The kit contains enough primary and secondary antibody to perform four Western blot experiments.Phospho-c-Kit antibodies detect endogenous levels of c-Kit when phosphorylated at the specified sites. c-Kit (D13A2) XP® Rabbit mAb detects endogenous levels of total c-kit receptor protein.

此抗体套装包含TCF1/TCF7 (C63D9) Rabbit mAb #2203、LEF1 (C12A5) Rabbit mAb #2230、TCF4/TCF7L2 (C48H11) Rabbit mAb #2569、TCF3/TCF7L1 (D15G11) Rabbit mAb #2883各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。TCF/LEF1 Antibody Sampler 试剂盒提供了经济合算的方式检测TCF/LEF1家族成员总蛋白。本试剂盒包含有足够4次western实验的一抗和二抗。本试剂盒中每个抗体均可以特异识别对应的内源性总蛋白并与其他家族成员没有交叉反应。TCF1 (C63D9) Rabbit mAb不识别缺乏氨基端β-catenin结合结构域的显性负亚型TCF1。LEF1 (C12A5) Rabbit mAb不识别由替代启动子产生的显性负亚型TCF1。The TCF/LEF1 Antibody Sampler Kit provides an economical means of detecting total protein from the TCF/LEF1 family members. The kit contains enough primary and secondary antibodies to perform four Western blots with each antibody.Each antibody in this kit recognizes endogenous levels of total protein for the specified target and does not cross-react with other family members. TCF1 (C63D9) Rabbit mAb does not recognize the dominant negative isoforms of TCF1 lacking the amino-terminal β-catenin binding domain. LEF1 (C12A5) Rabbit mAb does not recognize the dominant negative forms of LEF1 generated by an alternative promoter.