此抗体套装包含Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb #3073、c-Kit (D13A2) XP® Rabbit mAb #3074、Phospho-c-Kit (Tyr719) Antibody #3391各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。c-Kit Antibody Sampler Kit是检测特异位点磷酸化的c-Kit蛋白和总c-Kit蛋白水平的快速、经济的选择。本试剂盒的一抗和二抗足够四次免疫印记检测实验使用。磷酸化c-Kit的抗体在c-Kit的特殊位点被磷酸化时能够检测到内源c-Kit的表达。抗c-Kit的兔源单克隆抗体 (D13A2) XP® 能检测内源总c-kit蛋白的水平。The c-Kit Antibody Sampler Kit provides a fast and economical means of evaluating levels of c-Kit receptor protein phosphorylated at the specified sites, as well as total c-Kit receptor levels. The kit contains enough primary and secondary antibody to perform four Western blot experiments.Phospho-c-Kit antibodies detect endogenous levels of c-Kit when phosphorylated at the specified sites. c-Kit (D13A2) XP® Rabbit mAb detects endogenous levels of total c-kit receptor protein.

此抗体套装包含MAPKAPK-2 (D1E11) Rabbit mAb #12155、Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007、Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。MAPKAPK-2 Antibody Sampler Kit为评估特定位点磷酸化的MAPKAPK-2蛋白水平，以及总MAPKAPK-2蛋白水平提供了一个经济的方法。该试剂盒所提供的一抗和二抗可以完成四次Western blot实验。MAPKAPK-2 Antibody Sampler Kit中每个抗体都能检测它所对应的内源性靶点蛋白水平。活化状态的抗体只能检测所列物种中的磷酸化靶蛋白。MAPKAPK-2 Antibody不能与MAPKAPK-3 、MAPKAPK-5发生交叉反应。The MAPKAPK-2 Antibody Sampler Kit provides an economical means of evaluating levels of MAPKAPK-2 protein phosphorylated at specific sites, as well as total MAPKAPK-2 protein levels. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.Each antibody in the MAPKAPK-2 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. MAPKAPK-2 Antibody does not cross-react with MAPKAPK-3 or -5.

此抗体套装包含Atg12 Antibody (Human Specific) #2010、LC3B (D11) XP® Rabbit mAb #3868、LC3A (D50G8) XP® Rabbit mAb #4599各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Autophagosome Marker Antibody Sampler 试剂盒提供了一种经济有效的方法，能够检测细胞中积累的自噬体。这种试剂盒含有足够的一抗和二抗，每个一抗可以进行四次western blots。Autophagosome Marker Antibody Sampler 试剂盒中的每个抗体可以检测到内源性的目标总蛋白。Atg12抗体（人源特异性）能够识别游离的Atg12或Atg12-Atg15复合物。LC3A (D50G8) XP® Rabbit mAb 兔单抗和 LC3B (D11) XP® Rabbit mAb 兔单抗能够与其它LC3异构体发生交叉反应，并且对于II型LC3的反应要更强。The Autophagosome Marker Antibody Sampler Kit provides an economical means to investigate the accumulation of autophagosomes within the cell. The kit contains enough primary and secondary antibodies to perform four western blots per primary antibody.Each antibody in the Autophagosome Marker Antibody Sampler Kit detects endogenous levels of total respective protein. Atg12 Antibody (Human Specific) recognizes free Atg12 and the Atg12-Atg5 conjugate. LC3A (D50G8) XP® Rabbit mAb and LC3B (D11) XP® Rabbit mAb may cross-react with other LC3 isoforms and have stronger reactivity with the type II form.

此kit包含Oct-4A (C30A3) Rabbit mAb #2840、Sox2 (D6D9) XP® Rabbit mAb #3579、Nanog (D73G4) XP® Rabbit mAb #4903各20µl。StemLight多潜能性转录因子抗体试剂盒包括一系列能够检测ES和iPS细胞中主要转录网络中关键组分蛋白包括Oct-4, Nanog, 和Sox2的抗体。本试剂盒可用于示踪人胚胎干细胞（ES）和诱导的多潜能干细胞（iPS）的多潜能。失去这些标志性蛋白意味着多潜能性的丧失或细胞分化。试剂盒中组分已经优化为标准稀释度用于在免疫荧光分析中配套使用，但是这些组件也能用于其他实验——请参阅单独的使用说明以寻找具体建议。试剂足够进行160次反应体系为100ul的免疫荧光实验。Nanog (D73G4) XP® Rabbit mAb能够检测内源性的总Nanog蛋白。Oct-4A (C30A3) Rabbit mAb能够识别内源性的总Oct-4A蛋白。Sox2 (D6D9) XP® Rabbit mAb能够识别内源性的总Sox2蛋白。StemLight™ Pluripotency Transcription Factor Antibody Kit contains a panel of antibodies for the detection of Oct-4, Nanog, and Sox2, key components of the core pluripotency transcription network in embryonic stem (ES) and induced pluripotent stem (iPS) cells. The kit can be used to track the pluripotent potential of human ES or iPS cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis at a standard dilution, but components are also validated for use in other applications - please refer to individual datasheet information for application specific recommendations. Enough reagents are provided for 160 immunofluorescent assays based on a working volume of 100 μl.Nanog (D73G4) XP® Rabbit mAb detects endogenous levels of total Nanog protein. Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. Sox2 (D6D9) XP® Rabbit mAb detects endogenous levels of total Sox2 protein.

此kit包含TRA-1-81 (TRA-1-81) Mouse mAb #4745、TRA-1-60(S) (TRA-1-60(S)) Mouse mAb #4746、SSEA4 (MC813) Mouse mAb #4755 各20µl。StemLight™表面标记试剂盒包括一系列抗体，能够识别特异性表达于人多潜能干细胞表面的抗原。本试剂盒可用于示踪人胚胎干细胞（ES）和诱导的多潜能干细胞（iPS）的多潜能。失去这些标志性蛋白意味着多潜能性的丧失或细胞分化。本试剂盒所包含的成分已经提前优化使其也适用于免疫荧光分析。TRA-1-60(S) (TRA-1-60(S)) Mouse mAb鼠单抗可识别内源性神经氨酸酶敏感的TRA-1-60抗原蛋白。TRA-1-81 (TRA-1-81) Mouse mAb鼠单抗识别内源性的TRA-1-81抗原蛋白。SSEA4 (MC813) Mouse mAb鼠单抗识别内源性SSEA4抗原蛋白。The StemLight™ Surface Marker Kit contains a panel of antibodies for the detection of antigens that are specifically expressed on the surface of human pluripotent cells. The kit can be used to track the pluripotent potential of human embryonic stem (ES) or induced pluripotent (iPS) cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis.TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen. TRA-1-81 (TRA-1-81) Mouse mAb detects endogenous levels of TRA-1-81 antigen. SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen.

此抗体套装包含GCN5L2 (C26A10) Rabbit mAb #3305、PCAF (C14G9) Rabbit mAb #3378、Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771、CBP (D6C5) Rabbit mAb #7389各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Lysine Acetyltransferase Antibody Sampler Kit可以提供一种快速经济的方法来检测内源性赖氨酸乙酰转移酶的水平，包括Acetyl-CBP, CBP, GCN5L2, 和 PCAF。试剂盒提供的每种一抗都足够进行4次western blot实验。Lysine Acetyltransferase Antibody Sampler Kit中的每种抗体能够识别各自的内源性的目的蛋白。该抗体不会与其他家族成员产生交叉反应。The Lysine Acetyltransferase Antibody Sampler Kit provides an economical means to examine several lysine acetyltrasferases, including: Acetyl-CBP, CBP, GCN5L2, and PCAF. The kit contains enough primary antibody to perform four western blots per primary.Each antibody in the Lysine Acetyltransferase Antibody Sampler Kit recognizes endogenous levels of respective target protein. The antibodies do not cross-react with other family members.

kit包括Histone H3 (D1H2) XP® Rabbit mAb 499T*1、AIF (D39D2) XP® Rabbit mAb 5318T*1、Vimentin (D21H3) XP® Rabbit mAb 5741T*1、Anti-rabbit IgG, HRP-linked Antibody 7074P2*1和 MEK1/2 (D1A5) Rabbit mAb 8727T*1 。Cell Fractionation Antibody Sampler Kit中的每种抗体都能识别内源性水平的它们各自的目标蛋白。抗体与其他家族成员没有交叉反应。每种抗体都用Cell Fractionation Kit #9038进行了验证。Cell Fractionation Antibody Sampler Kit提供了一种经济的方法来检测每个分离的亚细胞成分的纯度。 Each antibody in the Cell Fractionation Antibody Sampler Kit recognizes endogenous levels of its respective target protein. The antibodies do not cross-react with other family members.The Cell Fractionation Antibody Sampler Kit provides an economical means for determining the purity of each distinctly separated subcellular fraction by western blot using Cell Signaling Technology's Cell Fractionation Kit #9038.

此抗体组合kit包含2402T*1、2404T*1、2405T*1、7074P2*1、7076P2*1、9709T*1。The HSP27 Antibody Kit provides an economical means to evaluate the activation status of the HSP27 protein. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.Each antibody in the HSP27 Antibody Sampler Kit recognizes endogenous levels of its specific target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site.Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27 and to full-length human HSP27 protein. Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser15 or Ser78 of human HSP27 protein. Antibodies are purified by protein A and peptide affinity chromatography.

此抗体套装包含Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818T、Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676T、Acetyl-CoA Carboxylase 1 Antibody #4190T、Anti-rabbit IgG, HRP-linked Antibody #7074P2、Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb #8578T。Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler试剂盒提供了一种经济的方式以区分两种acetyl-CoA carboxylase亚型，以及total acetyl-CoA carboxylase 和 phosphorylated acetyl-CoA carboxylase。该试剂盒提供了足以进行4次western blot分析的一抗。每个抗体都可以识别其内源性的靶蛋白。Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗 可以识别Ser79被磷酸化的内源性acetyl-CoA carboxylase。合成对应human acetyl-CoA carboxylase 1蛋白序列的肽段免疫动物获得多抗。多抗经过蛋白A和肽段亲和层析纯化获得。合成磷酸肽段免疫动物获得单抗，磷酸肽段和human acetyl-CoA carboxylase蛋白的Ser79及其邻近氨基酸序列一致，或和human acetyl-CoA carboxylase α1蛋白的Ser523或human acetyl-CoA carboxylase 2的Val1416获得单抗，磷酸肽段和human acetyl-CoA carboxylase蛋白的Ser79。The Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit provides an economical means of distinguishing between the two acetyl-CoA carboxylase isoforms, and between total acetyl-CoA carboxylase and phosphorylated acetyl-CoA carboxylase. The kit includes enough antibody to perform four western blot experiments per primary antibody.Each antibody recognizes endogenous levels of their target protein. Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human acetyl-CoA carboxylase 1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein, or a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1, or Val1416 of human acetyl-CoA carboxylase 2 protein.

该抗体套装含Notch1 (D1E11) XP® Rabbit mAb #3608T、Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147T、Notch3 (D11B8) Rabbit mAb #5276T、Notch2 (D76A6) XP® Rabbit mAb #5732T和Anti-rabbit IgG, HRP-linked Antibody #7074P2各一。Notch Isoform Antibody Sampler Kit提供经济合适的方式检测Notch信号通路。试剂盒含有足够做四次小的Western blot所需的一抗和二抗。Cleaved Notch1 (Val1744) (D3B8) XP® Rabbit mAb兔单抗只识别1743位甘氨酸和1744位缬氨酸之间被剪切的内源性Notch1蛋白的胞质结构域。抗体不识别全长Notch1蛋白或者被其他位点的剪切型Notch1。在不同细胞中存在Notch1的远处羧基末端的突变，因此此抗体识别大小不同的剪切型Notch1（见参考文献6）Notch1 (D6F11) XP® Rabbit mAb兔源抗体检测内源性的总Notch1蛋白。它识别全长(~300 kDa)和跨膜/胞内区域NTM(~120 kDa)。Notch2 (D67C8) XPTM兔源单抗识别内源性的Notch2蛋白。它识别全长(~300 kDa)和跨膜/胞内区域NTM(~110 kDa)。Notch3 (D11B8) Rabbit mAb兔抗体识别内源性的总Notch3蛋白。此抗体识别全长270kDa的Notch3和截短的蛋白(NTM)，包含短的胞外区域，跨膜区域和胞内区域（90kDa）。The Notch Isoform Antibody Sampler Kit provides an economical means to investigate Notch Signaling. The kit contains primary and secondary antibodies to perform four western mini-blots with each antibody.Cleaved Notch1 (Val1744) (D3B8) XP® Rabbit mAb detects endogenous levels of the cytosolic domain of Notch1 only when cleaved between Gly1743 and Val1744. The antibody does not recognize full length Notch1 or Notch1 cleaved at other positions. It detects cleaved Notch1 with different sizes in different cell lines due to mutations at the distant C-termini of Notch1 (see ref 6). Notch1 (D6F11) XP® Rabbit detects endogenous level of total notch1 protein. It recognizes both the full-length (~300 kDa) and the transmembrane/intracellular region NTM (~120 kDa). Notch2 (D67C8) XPTM Rabbit mAb detects endogenous levels of total Notch2 protein. It recognizes both the full-length (~ 300 kDa) and the transmembrane/intracellular region NTM (~110 kDa). Notch3 (D11B8) Rabbit mAb detects endogenous levels of total Notch3 protein. The antibody recognizes both full-length (FL) Notch3 at 270 kDa and a truncated protein (NTM) containing a short extracellullar region, the transmembrane domain and the intracellular region at 90 kDa.

该抗体套装包含Aurora A (D3E4Q) Rabbit mAb #14475、Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914T、Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079T、Aurora B/AIM1 Antibody #3094T和Anti-rabbit IgG, HRP-linked Antibody #7074P2。Aurora Antibody Sampler Kit为研究细胞周期G2/M期提供了一个经济的方式。该试剂盒内含足够4次western blot实验的一抗和二抗。Aurora Antibody Sampler Kit中的每个抗体都能够检测其内源性靶蛋白，且不与家族其他成员发生交叉反应。The Aurora Antibody Sampler Kit provides an economical means to investigate the G2/M phase of the cell cycle. The kit contains enough primary and secondary antibodies to perform four Western blots with each antibody.Each antibody in the Aurora Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.

此套装包含pCMV-Tag4A-mBad/GrpE 20µg，Phospho-Bad (Ser136) (D25H8) Rabbit mAb #4366、Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284、Bad (D24A9) Rabbit mAb #9239、Phospho-Bad (Ser155) Antibody #9297各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl.Phospho-Bad Antibody Sampler Kit 提供了一种经济的方法研究Bad蛋白在凋亡中的作用。试剂盒中所包含的一抗和二抗，足够用于四次Western blots免疫印记。Bad (D24A9) Rabbit mAb 兔单抗能够检测内源性的Bad总蛋白。Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗只能检测内源性的Ser112位点磷酸化的Bad。Ser112的系统命名法是基于小鼠蛋白序列。在人和大鼠中，其同工酶对应的分别是Ser75和Ser113位点。Phospho-Bad (Ser136) (185D10) Rabbit mAb 兔单抗能够检测免疫共沉淀的或者转染水平的Ser136 磷酸化的 Bad 蛋白。Ser136系统命名法是基于小鼠的蛋白序列，其同工蛋白在人和大鼠中的位点分别是Ser99和Ser137。Phospho-Bad (Ser155) Antibody 能够检测转染的Ser155位点磷酸化的Bad蛋白。Ser155 的系统命名法是基于小鼠的序列，其在人和大鼠中同工酶的位点分别是Ser118 和 Ser156 位点。The Phospho-Bad Antibody Sampler Kit provides an economical means to investigate the role of Bad protein in apoptosis. The kit contains primary and secondary antibodies to perform four Western blots with each antibody.Bad (D24A9) Rabbit mAb detects endogenous levels of total Bad protein. Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation sites are Ser75 in human and Ser113 in rat. Phospho-Bad (Ser136) (185D10) Rabbit mAb detects immunoprecipitated or transfected levels of Bad protein only when phosphorylated at Ser136. The Ser136 nomenclature is based upon the mouse sequence. The analogous phosphorylation sites are Ser99 in human and Ser137 in rat. Phospho-Bad (Ser155) Antibody detects transfected levels of Bad only when phosphorylated at Ser155. The Ser155 nomenclature is based upon the mouse sequence. The analogous phosphorylation sites are Ser118 in human and Ser156 in rat.

此抗体套装包含FAK (D2R2E) Rabbit mAb #13009、Phospho-FAK (Tyr576/577) Antibody #3281、Phospho-FAK (Tyr925) Antibody #3284、Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。FAK抗体样品试剂盒提供经济手段评估总的FAK蛋白水平，以及FAK在特异位点的磷酸化，试剂盒含有足够和第二抗体，每个抗体能做四次western blots。FAK抗体样品试剂盒中的每个抗体检测内源性靶标蛋白的水平，不典型与其他蛋白交叉反应。 激活状态抗体仅仅在指定残基磷酸化时识别靶标蛋白， 磷酸化FAK (酪氨酸397)抗体肯那个与过表达酪氨酸磷酸化的EGF受体交叉反应。The FAK Antibody Sampler Kit provides an economical means of evaluating total FAK protein levels as well as FAK phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.Each antibody in the FAK Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross react with other proteins. Activation state antibodies recognize target proteins only when phosphorylated at the indicated residue. Phospho-FAK (Tyr397) Antibody may cross-react with overexpressed tyrosine phosphorylated EGF receptors.

此抗体套装包含Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb #2992、Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb #3170、PDGF Receptor α (D1E1E) XP® Rabbit mAb #3174、Phospho-PDGF Receptor α (Tyr1018) Antibody #4547各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。PDGF Receptor α Antibody Sampler Kit为检测PDGF receptor α protein (PDGFRα)和特定位点磷酸化的PDGFRα提供了经济有效地手段。本试剂盒的一抗和二抗足够四次免疫印迹检测实验使用。PDGF Receptor α Antibody Sampler Kit中的每一种抗体能够检测其特异的靶分子在体内的表达水平，激活态抗体仅在特定位点磷酸化时识别靶蛋白。磷酸化PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) 兔单抗和 磷酸化PDGF Receptor α (Tyr1018) k抗体它激活的酪氨酸激酶蛋白发生交叉反应。PDGF Receptor α (D1E1E) XP® 兔单抗可能与高表达的PDGFRβ发生交叉反应。The PDGF Receptor α Antibody Sampler Kit provides an economical means of evaluating total PDGF receptor α protein (PDGFRα) levels as well as PDGFRα phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.Each antibody in the PDGF Receptor α Antibody Sampler Kit detects endogenous levels of its target protein, with activation state antibodies recognizing target proteins only when phosphorylated at the indicated residues. Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb and Phospho-PDGF Receptor α (Tyr1018) Antibody may cross-react with other activated tyrosine kinases; PDGF Receptor α (D1E1E) XP® Rabbit mAb may cross-react with PDGFRβ at overexpressed levels.

此抗体套装包含Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb #11989 、Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb #8753、Phospho-p90RSK (Thr573) Antibody #9346、RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355各20µl，并含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。p90RSK Antibody Sampler Kit提供了一个经济的方法评价特异位点磷酸化的p90RSK蛋白水平，以及总p90RSK蛋白水平。该试剂盒包含足够的一抗、二抗完成四个免疫印记实验。RSK1/RSK2/RSK3 (32D7) Rabbit mAb兔单抗能检测内源性总RSK1-3蛋白水平。每个针对活化状态的单克隆抗体都能识别它的特异性靶点，并可能与相关磷酸化的RSK蛋白发生交叉反应。The p90RSK Antibody Sampler Kit provides an economical means of evaluating levels of p90RSK protein phosphorylated at specific sites, as well as total p90RSK protein levels. The kit contains enough primary and secondary antibody to perform four Western blot experiments.RSK1/RSK2/RSK3 (32D7) Rabbit mAb detects endogenous levels of total RSK1-3 proteins. Each activation state monoclonal antibody recognizes its specific target; some cross-reactivity with related phosphorylated RSK proteins may occur.