Use 10X Orange Loading Dye as a loading buffer for DNA gel electrophoresis. This dye is not visualized when imaged on Odyssey Imagers. The primary application is electrophoretic mobility shift assays.

Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

WesternSure PREMIUM Chemiluminescent Substrate is a highly sensitive enhanced substrate for detecting horseradish peroxidase (HRP) on immunoblots. Sensitivity comparable with SuperSignal West Dura for greater than 60 minutes post-incubation;Most intense signal with longest duration;Ldeal for use with digital imaging systems. The substrate pack contains Luminol Enhancer Solution and Stable Peroxidase Solution.

不含哺乳动物蛋白，与哺乳动物抗体的交叉反应性更低；
即用型配方；
 针对Odyssey近红外荧光成像系统优化，同时也可以用于化学发光检测；
 适用于磷酸化指标检测。    

不含哺乳动物蛋白，与哺乳动物抗体的交叉反应性更低；
即用型配方；
针对Odyssey近红外荧光成像系统优化，同时也可以用于化学发光检测。

The Chameleon Vue Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for molecular weight estimation. The pre-stained protein ladder also: Provides a vibrant color scheme that allows you to quickly orient your membrane；Is compatible for use with WesternSure® Pen for chemiluminescent detection。 Optimized for use with Bis-Tris and Tris-Glycine gels. Use for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly. The ladder consists of:Nine recombinant protein bands (8 – 260 kDa)；One green reference band (8 kDa)；Three orange reference bands (38, 70, and 260 kDa)； Five blue reference bands (15, 25, 50, 90, and 125 kDa)。

IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength. This infrared dye is ideal for labeling proteins, peptides, and antibodies.The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

IRDye 800RS is an excellent choice for nucleic acid applications. IRDye 800RS is available as an NHS ester. 

Assays that use IRDye® 800CW conjugates (such as in vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.The carboxylate dye has no reactive group and cannot be used for labeling

IRDye 800CW Maleimide provides the functionality for labeling molecules that contain free sulfhydryl (-SH) groups with IRDye 800CW. The reactive group of the IRDye 800CW Maleimide allows conjugation reactions to be performed very efficiently at physiological pH. IRDye 800CW Maleimide will label molecules with free sulfhydryl (-SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging instruments with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively. The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

IRDye 750 is a symmetric dye with a peak excitation at 756 nm and maximum emission at 776 nm in 1X PBS. It is spectrally similar to other 750 dyes that are used for flow cytometry, microscopy and other applications. IRDye 750 Maleimide is an infrared dye with detection near 750 nm. Maleimide reactive group provides the functionality for labeling molecules that contain free sulfhydryl (-SH thiol) groups. This reactive group allows conjugation reactions to be performed at physiological pH.

IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength. This infrared dye is ideal for labeling proteins, peptides, and antibodies.IRDye® 680RD Maleimide will label molecules with free sulfhydryl (-SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

The first nonfluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500 – 800 nm).Used for FRET (fluorescence resonance energy transfer) applications such as protease assays.Efficient nonfluorescent quenching of near-infrared (NIR) dyes.Widest available quenching range – no need to carefully match the donor's fluorescence spectrum with the acceptor's absorbance.Pair IRDye QC-1 with any visible or near-infrared fluorophore within its wide wavelength range.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging instruments with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively.IRDye® 680LT Maleimide will label molecules with free sulfhydryl (—SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

Assays that use IRDye® 680RD conjugates (such as small animal imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.Carboxylate (non-reactive) form of IRDye 680RD is an ideal control. The carboxylate dye has no reactive group and cannot be used for labeling.

IRDye 750 is a symmetric dye with a peak excitation at 756 nm and maximum emission at 776 nm in 1X PBS. It is spectrally similar to other 750 dyes that are used for flow cytometry, microscopy and other applications. IRDye 750 NHS Ester is an infrared dye with detection near 750 nm. The NHS ester reactive group provides the functionality for labeling primary amino groups.

IRDye 800CW is ideal for protein and antibody labeling, or nucleic acid applications with high labeling density. The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

he Chameleon 800 Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for both visible and 800 channel near-infrared detection. Includes 6 protein bands from 8 to 260 kDa (see figure). Use for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly.

The Chameleon 700 Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for both visible and 700 channel near-infrared detection. Includes 7 protein bands from 8 to 260 kDa . Use for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly.