VRDye 蛋白标记试剂盒经过优化，可标记一抗或二抗，用于显微镜检查、流式细胞术、免疫组织化学 (IHC) 或其他需要荧光团偶联抗体的应用。该试剂盒还可用于标记分子量范围为 50 kDa 至 200 kDa 的蛋白质。试剂盒中包含的可见染料被 N-羟基琥珀酰亚胺 (NHS) 酯激活，这是标记蛋白质最常用的反应基团。 NHS 酯与伯胺反应，形成稳定的共价键。该试剂盒针对标记 100 µg 一抗或二抗 IgG 抗体进行了优化。
产品组份：
3 x 0.15 mg of VRDye 549 Reactive Dye (store at -20 °C)
0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C)
25 mL PBS (store at 4 °C)
0.5 mL Ultra Pure Water (store at 4 °C)
3 Zeba Desalting Spin Columns (store at 4 °C)

The minimum recommended protein molecular weight for these columns is 7 kDa. Pierce Zeba Desalting Spin Column instructions Protocol for VRDye Protein Labeling Kit – High MW-Microscale Quick Reference Guide.
VRDye™ Protein Labeling Kits – High MW-Microscale are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 100 µg of an IgG antibody.

IRDye 680LT Protein Labeling Kits can be used to label peptides, antibodies and other proteins for applications such as Western blots and microscopy. These dyes and labeling kits are not recommended for labeling small animal imaging agents or for performing In-Cell Western™ assays. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

VRDye 蛋白标记试剂盒经过优化，可标记一抗或二抗，用于显微镜检查、流式细胞术、免疫组织化学 (IHC) 或其他需要荧光团偶联抗体的应用。该试剂盒还可用于标记分子量范围为 50 kDa 至 200 kDa 的蛋白质。试剂盒中包含的可见染料被 N-羟基琥珀酰亚胺 (NHS) 酯激活，这是标记蛋白质最常用的反应基团。 NHS 酯与伯胺反应，形成稳定的共价键。该试剂盒针对标记 100 µg 一抗或二抗 IgG 抗体进行了优化。
产品组份：
3 x 0.15 mg of VRDye 490 Reactive Dye (store at -20 °C)
0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C)
25 mL PBS (store at 4 °C)
0.5 mL Ultra Pure Water (store at 4 °C)
3 x Pierce Zeba Desalting Spin Columns (store at 4 °C)

The minimum recommended protein molecular weight for these columns is 7 kDa. Pierce Zeba Desalting Spin Column instructions Protocol for VRDye Protein Labeling Kit – High MW-Microscale Quick Reference Guide.
VRDye™ Protein Labeling Kits – High MW-Microscale are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 100 µg of an IgG antibody.

IRDye® 800CW Protein Labeling Kits can be used to label peptides, antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. All LI-COR IRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

IRDye® 680RD Protein Labeling Kits can be used to label antibodies and other proteins for small animal imaging and microscopy applications. All LI-COR IRDye protein labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

IRDye® 680RD Protein Labeling Kits can be used to label antibodies and other proteins for small animal imaging and microscopy applications. All LI-COR IRDye protein labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

IRDye 680LT Protein Labeling Kits can be used to label peptides, antibodies and other proteins for applications such as Western blots and microscopy. These dyes and labeling kits are not recommended for labeling small animal imaging agents or for performing In-Cell Western™ assays. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

VRDye 蛋白标记试剂盒经过优化，可标记一抗或二抗，用于显微镜检查、流式细胞术、免疫组织化学 (IHC) 或其他需要荧光团偶联抗体的应用。该试剂盒还可用于标记分子量范围为 50 kDa 至 200 kDa 的蛋白质。试剂盒中包含的可见染料被 N-羟基琥珀酰亚胺 (NHS) 酯激活，这是标记蛋白质最常用的反应基团。 NHS 酯与伯胺反应，形成稳定的共价键。该试剂盒针对标记 100 µg 一抗或二抗 IgG 抗体进行了优化。
产品组份：
3 x 0.15 mg of VRDye 490 Reactive Dye (store at -20 °C)
0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C)
25 mL PBS (store at 4 °C)
0.5 mL Ultra Pure Water (store at 4 °C)
3 x Pierce® Zeba™ Desalting Spin Columns (store at 4 °C)

The minimum recommended protein molecular weight for these columns is 7 kDa. Pierce Zeba Desalting Spin Column instructions Protocol for VRDye Protein Labeling Kit – High MW Quick Reference Guide.
VRDye™ Protein Labeling Kits – High MW are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 1 mg of an IgG antibody.

IRDye® 800CW Protein Labeling Kits can be used to label peptides, antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. All LI-COR IRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

VRDye 蛋白标记试剂盒经过优化，可标记一抗或二抗，用于显微镜检查、流式细胞术、免疫组织化学 (IHC) 或其他需要荧光团偶联抗体的应用。该试剂盒还可用于标记分子量范围为 50 kDa 至 200 kDa 的蛋白质。试剂盒中包含的可见染料被 N-羟基琥珀酰亚胺 (NHS) 酯激活，这是标记蛋白质最常用的反应基团。 NHS 酯与伯胺反应，形成稳定的共价键。该试剂盒针对标记 1 mg 一抗或二抗 IgG 抗体进行了优化。
产品组份：
3 x 0.15 mg of VRDye 549 Reactive Dye (store at -20 °C)
0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C)
25 mL PBS (store at 4 °C)
0.5 mL Ultra Pure Water (store at 4 °C)
3 x Pierce® Zeba™ Desalting Spin Columns (store at 4 °C)

The minimum recommended protein molecular weight for these columns is 7 kDa. Pierce Zeba Desalting Spin Column instructions Protocol for VRDye Protein Labeling Kit – High MW Quick Reference Guide.
VRDye™ Protein Labeling Kits – High MW are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 1 mg of an IgG antibody.

IRDye 680LT Protein Labeling Kits can be used to label peptides, antibodies and other proteins for applications such as Western blots and microscopy. These dyes and labeling kits are not recommended for labeling small animal imaging agents or for performing In-Cell Western™ assays. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

IRDye® 680RD Protein Labeling Kits can be used to label antibodies and other proteins for small animal imaging and microscopy applications. All LI-COR IRDye protein labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

IRDye® 800CW Protein Labeling Kits can be used to label peptides, antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. All LI-COR IRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services. IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary. IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

VRDye™ Protein Labeling Kits – High MW-Microscale are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 100 µg of an IgG antibody.

VRDye™ Protein Labeling Kits – High MW are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 1 mg of an IgG antibody.