IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength. This infrared dye is ideal for labeling proteins, peptides, and antibodies.The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

IRDye 800RS is an excellent choice for nucleic acid applications. IRDye 800RS is available as an NHS ester. 

Assays that use IRDye® 800CW conjugates (such as in vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.The carboxylate dye has no reactive group and cannot be used for labeling

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging instruments with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively. The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

IRDye 750 is a symmetric dye with a peak excitation at 756 nm and maximum emission at 776 nm in 1X PBS. It is spectrally similar to other 750 dyes that are used for flow cytometry, microscopy and other applications. IRDye 750 Maleimide is an infrared dye with detection near 750 nm. Maleimide reactive group provides the functionality for labeling molecules that contain free sulfhydryl (-SH thiol) groups. This reactive group allows conjugation reactions to be performed at physiological pH.

IRDye 800CW Maleimide provides the functionality for labeling molecules that contain free sulfhydryl (-SH) groups with IRDye 800CW. The reactive group of the IRDye 800CW Maleimide allows conjugation reactions to be performed very efficiently at physiological pH. IRDye 800CW Maleimide will label molecules with free sulfhydryl (-SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength. This infrared dye is ideal for labeling proteins, peptides, and antibodies.IRDye® 680RD Maleimide will label molecules with free sulfhydryl (-SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

The first nonfluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500 – 800 nm).Used for FRET (fluorescence resonance energy transfer) applications such as protease assays.Efficient nonfluorescent quenching of near-infrared (NIR) dyes.Widest available quenching range – no need to carefully match the donor's fluorescence spectrum with the acceptor's absorbance.Pair IRDye QC-1 with any visible or near-infrared fluorophore within its wide wavelength range.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging instruments with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively.IRDye® 680LT Maleimide will label molecules with free sulfhydryl (—SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

Assays that use IRDye® 680RD conjugates (such as small animal imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.Carboxylate (non-reactive) form of IRDye 680RD is an ideal control. The carboxylate dye has no reactive group and cannot be used for labeling.

IRDye 750 is a symmetric dye with a peak excitation at 756 nm and maximum emission at 776 nm in 1X PBS. It is spectrally similar to other 750 dyes that are used for flow cytometry, microscopy and other applications. IRDye 750 NHS Ester is an infrared dye with detection near 750 nm. The NHS ester reactive group provides the functionality for labeling primary amino groups.

IRDye 800CW is ideal for protein and antibody labeling, or nucleic acid applications with high labeling density. The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

Alkyne groups preferentially label molecules containing azide groups (-N3). Copper-catalyzed Click Chemistry is used for initiating reactions between alkynes and azides. Within physiological temperature and pH ranges, the alkyne group does not react with amines or hydroxyls, which are naturally present in many biomolecules. However, at higher pH or upon exposure to short-wave ultraviolet (UV) light, the alkyne group may become unstable towards sulfhydryls (-SH, thiols), organophosphines such as tris(2-carboxylethyl)phosphine (TCEP), and dihalogens such as iodine (I2).

Alkyne groups preferentially label molecules containing azide groups (-N3). Copper-catalyzed Click Chemistry is used for initiating reactions between alkynes and azides. Within physiological temperature and pH ranges, the alkyne group does not react with amines or hydroxyls, which are naturally present in many biomolecules. However, at higher pH or upon exposure to short-wave ultraviolet (UV) light, the alkyne group may become unstable towards sulfhydryls (-SH, thiols), organophosphines such as tris(2-carboxylethyl)phosphine (TCEP), and dihalogens such as iodine (I2).

Azides preferentially label molecules containing either alkynes or DBCO groups. Copper-catalyzed Click Chemistry is used for initiating reactions between azides and alkynes, whereas azides spontaneously react with DBCO groups. Within physiological temperature and pH ranges, the azide group does not react with amines or hydroxyls, which are naturally present in many biomolecules. However, the azide group is unstable towards sulfhydryls (-SH, thiols) and organophosphines such as tris(2-carboxylethyl)phosphine (TCEP).

Azides preferentially label molecules containing either alkynes or DBCO groups. Copper-catalyzed Click Chemistry is used for initiating reactions between azides and alkynes, whereas azides spontaneously react with DBCO groups. Within physiological temperature and pH ranges, the azide group does not react with amines or hydroxyls, which are naturally present in many biomolecules. However, the azide group is unstable towards sulfhydryls (-SH, thiols) and organophosphines such as tris(2-carboxylethyl)phosphine (TCEP).

The dibenzocyclooctyne group (DBCO) allows Copper-free Click Chemistry to be done with live cells, whole organisms, and non-living samples. DBCO groups will preferentially and spontaneously label molecules containing azide groups (–N3). Within physiological temperature and pH ranges, the DBCO group does not react with amines or hydroxyls, which are naturally present in many biomolecules. Reaction of the DBCO group with the azide group is significantly faster than with the sulfhydryl group (–SH, thiol).

The dibenzocyclooctyne group (DBCO) allows Copper-free Click Chemistry to be done with live cells, whole organisms, and non-living samples. DBCO groups will preferentially and spontaneously label molecules containing azide groups (–N3). Within physiological temperature and pH ranges, the DBCO group does not react with amines or hydroxyls, which are naturally present in many biomolecules. Reaction of the DBCO group with the azide group is significantly faster than with the sulfhydryl group (–SH, thiol).

Assays that use IRDye® 800CW conjugates (such as in vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.The carboxylate dye has no reactive group and cannot be used for labeling

IRDye 750 is a symmetric dye with a peak excitation at 756 nm and maximum emission at 776 nm in 1X PBS. It is spectrally similar to other 750 dyes that are used for flow cytometry, microscopy and other applications. IRDye 750 NHS Ester is an infrared dye with detection near 750 nm. The NHS ester reactive group provides the functionality for labeling primary amino groups.

IRDye 800RS is an excellent choice for nucleic acid applications. IRDye 800RS is available as an NHS ester. 

IRDye 800CW Maleimide provides the functionality for labeling molecules that contain free sulfhydryl (-SH) groups with IRDye 800CW. The reactive group of the IRDye 800CW Maleimide allows conjugation reactions to be performed very efficiently at physiological pH. IRDye 800CW Maleimide will label molecules with free sulfhydryl (-SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

IRDye 800CW is ideal for protein and antibody labeling, or nucleic acid applications with high labeling density. The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

The dibenzocyclooctyne group (DBCO) allows Copper-free Click Chemistry to be done with live cells, whole organisms, and non-living samples. DBCO groups will preferentially and spontaneously label molecules containing azide groups (–N3). Within physiological temperature and pH ranges, the DBCO group does not react with amines or hydroxyls, which are naturally present in many biomolecules. Reaction of the DBCO group with the azide group is significantly faster than with the sulfhydryl group (–SH, thiol).

The dibenzocyclooctyne group (DBCO) allows Copper-free Click Chemistry to be done with live cells, whole organisms, and non-living samples. DBCO groups will preferentially and spontaneously label molecules containing azide groups (–N3). Within physiological temperature and pH ranges, the DBCO group does not react with amines or hydroxyls, which are naturally present in many biomolecules. Reaction of the DBCO group with the azide group is significantly faster than with the sulfhydryl group (–SH, thiol).

IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength. This infrared dye is ideal for labeling proteins, peptides, and antibodies.The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging instruments with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively. The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

IRDye 750 is a symmetric dye with a peak excitation at 756 nm and maximum emission at 776 nm in 1X PBS. It is spectrally similar to other 750 dyes that are used for flow cytometry, microscopy and other applications. IRDye 750 Maleimide is an infrared dye with detection near 750 nm. Maleimide reactive group provides the functionality for labeling molecules that contain free sulfhydryl (-SH thiol) groups. This reactive group allows conjugation reactions to be performed at physiological pH.

Azides preferentially label molecules containing either alkynes or DBCO groups. Copper-catalyzed Click Chemistry is used for initiating reactions between azides and alkynes, whereas azides spontaneously react with DBCO groups. Within physiological temperature and pH ranges, the azide group does not react with amines or hydroxyls, which are naturally present in many biomolecules. However, the azide group is unstable towards sulfhydryls (-SH, thiols) and organophosphines such as tris(2-carboxylethyl)phosphine (TCEP).

Azides preferentially label molecules containing either alkynes or DBCO groups. Copper-catalyzed Click Chemistry is used for initiating reactions between azides and alkynes, whereas azides spontaneously react with DBCO groups. Within physiological temperature and pH ranges, the azide group does not react with amines or hydroxyls, which are naturally present in many biomolecules. However, the azide group is unstable towards sulfhydryls (-SH, thiols) and organophosphines such as tris(2-carboxylethyl)phosphine (TCEP).