The Quick Western Kit provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step. This reduces the overall time to complete a Western blot and provides the advantages of near-infrared fluorescence detection. The kit also serves as a detection solution for post-immunoprecipitation samples by Western blot because it does not bind to denatured mouse monoclonal or rabbit monoclonal antibodies. (See Detection of Post-IP Proteins with Western blots using the Quick Western Kit). The key benefit is the ability to use the same antibody for immunoprecipitation and post-immunoprecipitation detection by Western blot. Kit Components： IRDye 680RD Detection Reagent Intercept® (PBS) Blocking Buffer Quick Reference Card

Kit Components： IRDye® 800CW Goat anti-Mouse Secondary Antibody (0.1 mg, 1 mg/mL) IRDye 680RD Goat anti-Rabbit Secondary Antibody (0.1 mg, 1 mg/mL) 500 mL Intercept® (PBS) Blocking Buffer 10 Immobilon®-FL PVDF Membranes (0.45 µm, 10 cm x 10 cm)

Kit Components： IRDye® 800CW Goat anti-Rabbit Secondary Antibody (0.1 mg, 1 mg/mL) IRDye 680RD Goat anti-Mouse Secondary Antibody (0.1 mg, 1 mg/mL) 500 mL Intercept® (PBS) Blocking Buffer 10 Immobilon®-FL PVDF Membranes (0.45 µm, 10 cm x 10 cm)

Kit Components： IRDye® 800CW Goat anti-Mouse Secondary Antibody (0.1 mg, 1 mg/mL) IRDye 680RD Goat anti-Rabbit Secondary Antibody (0.1 mg, 1 mg/mL) 500 mL Intercept® (PBS) Blocking Buffer 10 Odyssey Nitrocellulose Membranes (0.22 µm, 7 cm x 8.5 cm)

Kit Components： IRDye® 800CW Goat anti-Rabbit Secondary Antibody (0.1 mg, 1 mg/mL) IRDye 680RD Goat anti-Mouse Secondary Antibody (0.1 mg, 1 mg/mL) 500 mL Intercept® (PBS) Blocking Buffer 10 Odyssey Nitrocellulose Membranes (0.22 µm, 7 cm x 8.5 cm)

Kit Components： IRDye® 800CW Goat anti-Mouse Secondary Antibody (0.1 mg, 1 mg/mL) IRDye 680RD Goat anti-Rabbit Secondary Antibody (0.1 mg, 1 mg/mL) 500 mL Intercept® (TBS) Blocking Buffer 10 Immobilon®-FL PVDF Membranes (0.45 µm, 10 cm x 10 cm)

Kit Components： IRDye® 800CW Goat anti-Rabbit Secondary Antibody (0.1 mg, 1 mg/mL) IRDye 680RD Goat anti-Mouse Secondary Antibody (0.1 mg, 1 mg/mL) 500 mL Intercept® (TBS) Blocking Buffer 10 Immobilon®-FL PVDF Membranes (0.45 µm, 10 cm x 10 cm)

Kit Components： IRDye® 800CW Goat anti-Mouse Secondary Antibody (0.1 mg, 1 mg/mL) IRDye 680RD Goat anti-Rabbit Secondary Antibody (0.1 mg, 1 mg/mL) 500 mL Intercept® (TBS) Blocking Buffer 10 Odyssey Nitrocellulose Membranes (0.22 µm, 7 cm x 8.5 cm)

Kit Components： IRDye® 800CW Goat anti-Rabbit Secondary Antibody (0.1 mg, 1 mg/mL) IRDye 680RD Goat anti-Mouse Secondary Antibody (0.1 mg, 1 mg/mL) 500 mL Intercept® (TBS) Blocking Buffer 10 Odyssey Nitrocellulose Membranes (0.22 µm, 7 cm x 8.5 cm)

The non-mammalian Intercept® Blocker in these kits is in PBS (phosphate-buffered saline) containing 0.06% Kathon II. The Immobilon®-FL PVDF membrane included in these kits has been tested to ensure a low-background for infrared Western blot success. The Single Buffer Kit (P/N 926-32098) contains: Intercept (PBS) Blocking Buffer, 500 mL Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m)

The non-mammalian Intercept® Blocker in these kits is in TBS (Tris-buffered saline) containing 0.06% Kathon II. The Immobilon®-FL PVDF membrane included in these kits has been tested to ensure a low background for infrared Western blot success. The Single Buffer Kit (P/N 926-32099) contains: Intercept (TBS) Blocking Buffer, 500 mL Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m)

The non-mammalian Intercept® Blocker in these kits is in TBS (Tris-buffered saline) containing 0.06% Kathon II. The Immobilon®-FL PVDF membrane included in these kits has been tested to ensure a low background for infrared Western blot success. The Bulk Buffer Kit (P/N 926-32100) contains: Intercept (TBS) Blocking Buffer, 10 x 500 mL Millipore Immobilon FL PVDF Membrane (1 roll, 0.45 µm; 26.5 cm x 3.75 m)

The non-mammalian Intercept® Blocker in these kits is in PBS (phosphate-buffered saline) containing 0.06% Kathon II. The Immobilon®-FL PVDF membrane included in these kits has been tested to ensure a low-background for infrared Western blot success. The Bulk Buffer Kit (P/N 829-32080) contains: Intercept (PBS) Blocking Buffer, 10 x 500 mL Millipore Immobilon-FL PVDF Membrane (1 roll, 0.45 µm; 26.5 cm x 3.75m)