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                  10X DNA 上样缓冲液:10X ORANGE LOADING DYE 3 ml 3 mL

                  价格:

                  货号:927-10100
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  Use 10X Orange Loading Dye as a loading buffer for DNA gel electrophoresis. This dye is not visualized when imaged on Odyssey Imagers. The primary application is electrophoretic mobility shift assays.

                  HIF-1 IRDYE 700 近红外荧光寡核苷酸探针:IRDye 700 HIF-1 Consensus Oligonucleotide 25 μl of 50 nm dsDNA 25 uL

                  价格:

                  货号:829-07929
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

                  AP-1 IRDYE 700 近红外荧光寡核苷酸探针:IRDye 700 AP-1 Consensus Oligonucleotide 25 μl of 50 nm dsDNA 25 uL

                  价格:

                  货号:829-07925
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

                  STAT3 IRDYE 700 近红外荧光寡核苷酸探针:IRDye 700 STAT3 Consensus Oligonucleotide 25 μl of 50 nm dsDNA 25 uL

                  价格:

                  货号:829-07922
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

                  p53 IRDYE 700近红外荧光寡核苷酸探针:IRDye 700 p53 Consensus Oligonucleotide 25 μl of 50 nm dsDNA 25 uL

                  价格:

                  货号:829-07921
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

                  NFkB IRDYE 700 近红外荧光寡核苷酸探针:IRDye 700 NFkB Consensus Oligonucleotide 25 μl of 50 nm dsDNA 25 uL

                  价格:

                  货号:829-07924
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

                  CREB IRDYE 700 近红外荧光寡核苷酸探针:IRDye 700 CREB Consensus Oligonucleotide 25 μl of 50 nm dsDNA 25 uL

                  价格:

                  货号:829-07923
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

                  Sp-1 IRDYE 700 近红外荧光寡核苷酸探针:IRDye 700 Sp-1 Consensus Oligonucleotide 25 μl of 50 nm dsDNA 25 uL

                  价格:

                  货号:829-07926
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

                  Odyssey® 凝胶迁移率实验( EMSA)试剂盒:ODYSSEY EMSA BUFFER KIT 1 kit

                  价格:

                  货号:829-07910
                  品牌:Licor

                  经销:基因生物技术国际贸易(上海)有限公司

                  通过用 IRDye®红外染料末端标记的寡核苷酸替换现有的 DNA 寡核苷酸,迁移率变动分析方案可以轻松转换为红外荧光分析。结合和电泳条件与任何其他 EMSA 检测方法相同。
                  用 IRDye 700 红外染料末端标记的 DNA 寡核苷酸是蛋白质结合的良好底物。使用 Odyssey®红外成像系统,IRDye 红外染料标记的 DNA 检测在 9.1 fmol 至 0.18 fmol 的 50 倍稀释范围内呈线性。Odyssey EMSA 试剂盒与 IRDye 700 EMSA 寡核苷酸相结合用于 EMSA 分析和可视化,是放射性同位素和化学发光检测方法的绝佳替代方法。使用 IRDye EMSA 试剂,可以在不到两小时内完成检测,无需凝胶转移或胶片曝光。凝胶甚至不需要从玻璃板中取出来进行成像。如果您对电泳进展不够满意,可以将凝胶放回电泳单元并运行更长时间。
                  试剂盒组成:
                  10X Binding Buffer (100 mM Tris, 500 mM KCl, 10 mM DTT, pH 7.5), 500 μL
                  25 mM DTT, 2.5% Tween® 20, 500 µL
                  Poly (dI•dC), 1 µg/µL in 10 mM Tris, 1 mM EDTA, pH 7.5, 125 µL
                  Sheared salmon sperm DNA, 0.5 µg/µL in 10 mM Tris, 1 mM EDTA, pH 7.5, 125 µL
                  50% Glycerol, 500 µL
                  1% NP-40, 500 µL
                  1 M KCl, 500 µL
                  100 mM MgCl2, 500 µL
                  200 mM EDTA, pH 8.0, 500 µL
                  10X Orange Loading Dye, 500 µL
                  储存条件:-20℃

                  Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method. A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

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