The CellVue Burgundy cell linker kit uses proprietary membrane labeling technology to stably incorporate a fluorescent dye with long aliphatic tails (CellVue Burgundy) into lipid regions of the cell membrane. The labeling vehicle provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The pattern of staining is dependent upon the cell type being labeled and the membranes of the cells. CellVue products have been reported to be useful for short-term in vitro cell proliferation studies, cell tracking in isolated organ preparation applications and cell tracking using the Pearl® Small Animal Imaging System or Odyssey® CLx or Odyssey Classic Infrared Imaging System.

The CellVue Burgundy cell linker kit uses proprietary membrane labeling technology to stably incorporate a fluorescent dye with long aliphatic tails (CellVue Burgundy) into lipid regions of the cell membrane. The labeling vehicle provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The pattern of staining is dependent upon the cell type being labeled and the membranes of the cells. CellVue products have been reported to be useful for short-term in vitro cell proliferation studies, cell tracking in isolated organ preparation applications and cell tracking using the Pearl® Small Animal Imaging System or Odyssey® CLx or Odyssey Classic Infrared Imaging System.

The CellVue NIR815 cell linker kit uses proprietary membrane labeling technology to stably incorporate a fluorescent dye with long aliphatic tails (CellVue NIR815) into lipid regions of the cell membrane. The labeling vehicle provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The pattern of staining is dependent upon the cell type being labeled and the membranes of the cells. CellVue NIR815, a near-infrared fluorescent cell linker has been reported to be useful for short-term in vitro cell proliferation studies, cell tracking in isolated organ preparation applications and cell tracking using the Pearl® Small Animal Imaging System or Odyssey® CLx or Odyssey Classic Infrared Imaging System.

IRDye® 800CW 2-DG is a fluorescent optical imaging agent that has been shown to be reactive with implanted tumors derived from a number of cell lines including A431, SW620, 3T3-L1, and PC3LMN4. An example is shown in Figure 1. The optical agent exhibits the expected dose response with increasing concentrations of the agent in both cell based and animal experiments. Uptake of the agent can be effectively blocked with either unlabeled 2-DG or glucose, confirming its specificity. Confocal microscopy demonstrated conclusively that IRDye® 800CW 2-DG is taken up by the cell and localized in the cytoplasm. While the exact mechanism of IRDye® 800CW 2-DG uptake is not known, there is strong evidence for the involvement of the GLUT transporter system.

IRDye® 800CW BoneTag Optical Probe and IRDye 680RD BoneTag Optical Probe are calcium-chelating compounds conjugated to IRDye 800CW or IRDye 680RD near-infrared dye. Use of IRDye NIR dyes extends fluorescence signal detection to the NIR region of the spectrum without affecting the compound’s ability to function as a marker of the mineralization process. NIR fluorescence detection improves depth of penetration due to low tissue autofluorescence, translating to low background interference.The ability to visualize bone anatomy and structures for an extended period facilitates use of the IRDye BoneTag in conjunction with a second optical agent specific for a primary target (i.e, tumor tissue). In addition, IRDye 800CW BoneTag may prove effective for studies dealing with bone metastasis and bone remodeling studies.

PSVue 794 is a near-infrared fluorescent probe for detection of apoptotic and necrotic cells, bacteria and other anionic membranes.The PSVue 794 reagent kit contains components to provide a 1 mM solution of PSVue 794 in aqueous solution. The compound exhibits fluorescence excitation maximum at 794nm and emission maximum at 810 nm and through its zinc(II)-dipicolylamine (Zn-DPA) moiety, it has been found to bind strongly to negatively charged bacterial cell walls (e.g. S. aureus, E. coli) and necrotic regions present in various tumors (e.g. mammary, prostate, glioma) in vitro and in vivo. In particular, it has also been found to bind to the phosphatidylserine (PS) residues exposed on the cell surface of apoptotic cells, making it a more cost-effective alternative to fluorescently labeled Annexin V in various cell death assays. The labeling vehicle provided with the kit (Diluent X) is designed to maximize dye solubility and is suitable for in vitro and in vivo use.

IRDye® 800CW RGD Optical Probe is a BrightSite™ near-infrared (NIR) fluorescently-labeled RGD imaging agent specifically designed to target the overexpression of integrins on tumors. Integrins are cell surface heterodimeric glycoproteins involved in cell-to-cell and cell-to-matrix interactions. Integrins are cell surface heterodimeric glycoproteins involved in cell-to-cell and cell-to-matrix interactions. The recognition motif, RGD (Arg-Gly-Asp), is a tripeptide sequence used to bind integrin receptors including αvbeta;3. Interest in using a labeled RGD peptide ligand for the study and/or monitoring of diseases related to the αvbeta;3 receptor over-expression is increasing. Several groups have labeled RGD successfully with fluorescent dyes for in vitro and in vivo imaging.

IRDye® 800CW EGF Optical Probe is a BrightSite™ near-infrared (NIR) fluorescently-labeled recombinant human epidermal growth factor (EGF). Epidermal growth factor receptor (EGFR) is one of a family of receptor tyrosine kinases found on the surface of epithelial cells, to which EGF binds. Many types of cancer cells have abnormally high EGFR levels on the cell surface. In vivo assays of EGF binding have proven effective in monitoring specific solid tumor models that exhibit overexpression of EGFR. EGF conjugated to the LI-COR NIR fluorophore, IRDye 800CW provides a versatile probes that can be used for in vitro and in vivo assays, as well as whole organ and tissue section analysis.

IRDye® 800CW BoneTag Optical Probe and IRDye 680RD BoneTag Optical Probe are calcium-chelating compounds conjugated to IRDye 800CW or IRDye 680RD near-infrared dye. Use of IRDye NIR dyes extends fluorescence signal detection to the NIR region of the spectrum without affecting the compound’s ability to function as a marker of the mineralization process. NIR fluorescence detection improves depth of penetration due to low tissue autofluorescence, translating to low background interference.The ability to visualize bone anatomy and structures for an extended period facilitates use of the IRDye BoneTag in conjunction with a second optical agent specific for a primary target (i.e, tumor tissue).

Enhanced permeability and retention (EPR) is a common characteristic of tumor vasculature. The vascular endothelium in the tumor microenvironment is often discontinuous, allowing molecules to diffuse into the surrounding tumor tissue. In addition to EPR, the lymphatic drainage for these regions is poor . Hence, larger molecules tend to accumulate. IRDye 800CW PEG Contrast Agent (25-60 kDa) is a non-specific imaging agent intended to exploit EPR in tumor biology.