快速,精准,方便的蛋白检测试剂盒,包含内参BSA。
Fast, sensitive, and convenient assay for protein quantification.
• Highest sensitivity and flexibility;
• Reducing agent and detergent compatible;
• Fast procedure allows protein quantification in only 40 min;
• The perfect supplement for NucleoSpin® TriPrep, NucleoSpin® RNA/Protein, and NucleoSpin® miRNA;
• Reference protein (BSA) included.
0.5 ml Reagent A (avidin)
0.5 ml Reagent B (biotinylated-HRP (horseradish peroxidase))
Sufficient for 200 slides
Recommended when using the Avidin-Biotin Complex (ABC) staining method for immunoperoxidase staining of tissues (IHC) or cells (ICC)
ImmunoCruz® ABC Kit provides increased sensitivity with decreased background
Recommended for use with conventional species-specific biotinylated secondary antibodies
Note: this kit is not species-specific. Secondary antibody is not included.
For complementary reagents to complete your ABC staining protocol, see also: UltraCruz® Blocking Reagent (sc-516214), Hydrogen Peroxide (sc-203336) and DAB, 50X (sc-24982)
Store at 4° C
快速,精准,方便的蛋白检测试剂盒,包含内参BSA。
Fast, sensitive, and convenient assay for protein quantification.
• Highest sensitivity and flexibility;
• Reducing agent and detergent compatible;
• Fast procedure allows protein quantification in only 40 min;
• The perfect supplement for NucleoSpin® TriPrep, NucleoSpin® RNA/Protein, and NucleoSpin® miRNA;
• Reference protein (BSA) included.
该检测试剂是为标准的western blotting 应用中检测化学发光的蛋白而设计的。蛋白和生物素化的分子量标记(已提供)可以在SDS-PAGE中分离,再被转移到膜上。与抗血清的一抗和辣根过氧化物酶(HRP) 标记的二抗孵育后,HRP标记的抗生物素抗体被结合,然后与LumiGLO ® 试剂反应,发出的光随后被捕获到X胶片上。The Phototope®-HRP Western Blot Detection System is designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film.