该检测试剂是为标准的western blotting 应用中检测化学发光的蛋白而设计的。蛋白和生物素化的分子量标记（已提供）可以在SDS-PAGE中分离，再被转移到膜上。与抗血清的一抗和辣根过氧化物酶（HRP） 标记的二抗孵育后，HRP标记的抗生物素抗体被结合，然后与LumiGLO ® 试剂反应，发出的光随后被捕获到X胶片上。The Phototope®-HRP Western Blot Detection System is designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film.