产地：USA (美国)
存储条件：RT, 2-8 & -20
VRDye 蛋白标记试剂盒经过优化，可标记一抗或二抗，用于显微镜检查、流式细胞术、免疫组织化学 (IHC) 或其他需要荧光团偶联抗体的应用。该试剂盒还可用于标记分子量范围为 50 kDa 至 200 kDa 的蛋白质。试剂盒中包含的可见染料被 N-羟基琥珀酰亚胺 (NHS) 酯激活，这是标记蛋白质最常用的反应基团。 NHS 酯与伯胺反应，形成稳定的共价键。该试剂盒针对标记 100 µg 一抗或二抗 IgG 抗体进行了优化。
产品组份：
3 x 0.15 mg of VRDye 549 Reactive Dye (store at -20 °C)
0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C)
25 mL PBS (store at 4 °C)
0.5 mL Ultra Pure Water (store at 4 °C)
3 Zeba Desalting Spin Columns (store at 4 °C)

The minimum recommended protein molecular weight for these columns is 7 kDa. Pierce Zeba Desalting Spin Column instructions Protocol for VRDye Protein Labeling Kit – High MW-Microscale Quick Reference Guide.
VRDye™ Protein Labeling Kits – High MW-Microscale are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 100 µg of an IgG antibody.