产地：USA (美国)
存储条件：RT, 2-8 & -20
VRDye 蛋白标记试剂盒经过优化，可标记一抗或二抗，用于显微镜检查、流式细胞术、免疫组织化学 (IHC) 或其他需要荧光团偶联抗体的应用。该试剂盒还可用于标记分子量范围为 50 kDa 至 200 kDa 的蛋白质。试剂盒中包含的可见染料被 N-羟基琥珀酰亚胺 (NHS) 酯激活，这是标记蛋白质最常用的反应基团。 NHS 酯与伯胺反应，形成稳定的共价键。该试剂盒针对标记 100 µg 一抗或二抗 IgG 抗体进行了优化。
产品组份：
3 x 0.15 mg of VRDye 490 Reactive Dye (store at -20 °C)
0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C)
25 mL PBS (store at 4 °C)
0.5 mL Ultra Pure Water (store at 4 °C)
3 x Pierce® Zeba™ Desalting Spin Columns (store at 4 °C)

The minimum recommended protein molecular weight for these columns is 7 kDa. Pierce Zeba Desalting Spin Column instructions Protocol for VRDye Protein Labeling Kit – High MW Quick Reference Guide.
VRDye™ Protein Labeling Kits – High MW are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.The kits are optimized for labeling 1 mg of an IgG antibody.