biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.
The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
biotechrabbit™ GenUP PCR Cleanup Kit has been developed for quick and easy cleanup or concentration of PCR fragments from reaction mixtures. High-yield PCR product with excellent quality is purified from the PCR mixtures in a simple two-step procedure. DNA is bound to a Mini Filter using a novel buffer and eluted. The need for a washing step has been eliminated, reducing hands-on-time. The procedure takes approximately 3 min, compared to 8 min required to other kits. The purified DNA is ready for use in all demanding molecular biology applications, including restriction digestion, ligation and sequencing.
biotechrabbit™ GenUP PCR/Gel Cleanup Kit has been specially developed for a quick and easy cleanup or concentration of PCR fragments from reaction mixtures as well as extraction of DNA from both TAE and TBE agarose gels. The DNA is bound to a Mini Filter using a novel buffer, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including restriction digestion, ligation, sequencing, transfection into mammalian cells and in vitro transcription.
biotechrabbit™ GenUP Blood DNA Kit is designed for fast isolation of genomic DNA from up to 400 µl whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. After an efficient lysis step, genomic DNA is bound to a Mini Filter, washed and eluted. The isolation chemistry and extraction protocol are optimized for maximum yield. Including lysis, isolated DNA is available in approximately 24 min. The isolated DNA is suitable for a wide range of different molecular biology applications.
Protocols are available for isolating DNA from 200 µl or 400 µl whole blood samples.
The GenUP Blood DNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP gDNA Kit (cat. no. BR0700601), GenUP Bacteria gDNA Kit (cat. no. BR0700701), GenUP Plant DNA Kit (cat. no. BR0700801) or GenUP Virus DNA/RNA Kit (cat. no. BR0701101).
biotechrabbit GenUP™ gDNA Kit is designed for a fast and efficient genomic DNA isolation from various sources and different amounts of starting material, such as mammalian tissues (including paraffin-embedded), buccal swabs and eukaryotic cell cultures. After a few initial procedures, the genomic DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. High yields of pure, unshared genomic DNA are ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
biotechrabbit™ GenUP Bacterial gDNA Kit has been specially developed for quick and easy purification of bacterial genomic DNA from both Gram-negative and difficult to process Gram-positive bacteria.
A combined lysozyme and proteolytic lysis steps allow efficient cell disruption. The DNA is bound to a high-capacity filter, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including PCR, enzymatic digestions, cloning and other.
biotechrabbit™ GenUP Plant DNA Kit has been specially developed for quick and easy purification of genomic DNA from a wide variety of plant materials, including fresh, frozen or dried samples from leaves, roots, stems and flowers.
The kit includes an advanced prefiltration step to remove unlysed tissue. Subsequently, DNA is bound to a Mini Filter and is subsequently washed and eluted in a separate tube. The purified DNA is ready for use in any demanding molecular biology application, including PCR, enzymatic digestions and cloning.
This kit provides three buffers for optimized processing with different plant materials. To determine optimal lysis conditions, side-by-side preparation using the three provided protocols are prepared.
biotechrabbit™ GenUP FFPE Paraffin Removal Solution is an odor-free, biofriendly and innovative solution to deparaffinize FFPE slices within minutes in a single step. This product replaces conventional procedures that involve toxic and/or flammable solvents. After removal of paraffin from FFPE slices with GenUP FFPE Paraffin Removal Solution, continue with the genomic DNA or RNA purification kit of your choice.
biotechrabbit™ GenUP Blood RNA Kit is designed for fast isolation of total RNA from up to 1 ml whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. Erythrocytes are removed in an initial lysis step. After an second lysis step, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. Including lysis, isolated RNA is available in approximately 45 min. The isolated RNA is suitable for a wide range of different molecular biology applications, including RT-PCR.
The GenUP Blood RNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP Total RNA Kit (BR07009), GenUP Virus RNA Kit (BR07010), GenUP Virus DNA/RNA Kit (BR07011) or UPzol RNA Isolation Solution (BR07001).
biotechrabbit™ GenUP Total RNA Kit has been specially developed for a quick and easy purification of total RNA from eukaryotic cell suspensions, tissues and biopsies, Gram-negative (e.g., E. coli) and Gram-positive bacteria and other sources. After few initial procedures, the RNA is bound to a filter, washed and then eluted in a separate tube. DNA is removed physically by binding to a filter without any DNAse treatment or the use of toxic β‑mercaptoethanol. The purified RNA is ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis and others.
biotechrabbit™ GenUP Virus DNA/RNA Kit has been specially developed for quick and easy isolation of viral RNA and DNA. The kit is especially useful when the origin of the virus is unknown. Viral double-stranded DNA and single-stranded RNA are simultaneously isolated from eukaryotic samples, including plasma, serum, and other body fluids as well as cell cultures, tissues, and buccal swabs.
The unique binding membrane of our high-capacity Mini Filters guaranties high yields. A high concentration of purified nucleic acid can be achieved with flexible elution volumes. The kit includes carrier RNA.
After a few initial procedures, the viral nucleic acids are bound to a Mini Filter, washed and then eluted in a separate tube. The purified nucleic acids are ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis, qPCR and RT-PCR.
biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.
The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Heat Labile Exonuclease 1 (HL-ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.
Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 3' - 5' exonuclease activity of Exonuclease 1 degrades single-stranded primers.
biotechrabbit™ GenUP Plant RNA Kit has been developed for quick and easy purification of total RNA from plant materials. After initial homogenization and lysis, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. The purified RNA is ready for use in any demanding molecular biology application, including RT-PCR.
Two lysis buffers, Buffer LYSIS LR and Buffer LYSIS LT, are provided to maximize yield. Most plant material can be processed with Buffer LYSIS LR. In the cases that yield using Buffer LYSIS LR is low, use Buffer LYSIS LT.
biotechrabbit™ GenUP Micro RNA Kit is designed for high yields of small RNA molecules and total RNA, including snRNA, miRNA, siRNA, tRNA, rRNA and mRNA, without the use of highly toxic β-mercaptoethanol. After using well established filter-technology to selectively remove genomic DNA, the RNA is bound, washed and eluted from the filter membrane using RNase-free water.
biotechrabbit™ GenUP PCR Cleanup Kit has been developed for quick and easy cleanup or concentration of PCR fragments from reaction mixtures. High-yield PCR product with excellent quality is purified from the PCR mixtures in a simple two-step procedure. DNA is bound to a Mini Filter using a novel buffer and eluted. The need for a washing step has been eliminated, reducing hands-on-time. The procedure takes approximately 3 min, compared to 8 min required to other kits. The purified DNA is ready for use in all demanding molecular biology applications, including restriction digestion, ligation and sequencing.
biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.
The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
biotechrabbit™ GenUP PCR/Gel Cleanup Kit has been specially developed for a quick and easy cleanup or concentration of PCR fragments from reaction mixtures as well as extraction of DNA from both TAE and TBE agarose gels. The DNA is bound to a Mini Filter using a novel buffer, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including restriction digestion, ligation, sequencing, transfection into mammalian cells and in vitro transcription.
biotechrabbit™ GenUP Blood DNA Kit is designed for fast isolation of genomic DNA from up to 400 µl whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. After an efficient lysis step, genomic DNA is bound to a Mini Filter, washed and eluted. The isolation chemistry and extraction protocol are optimized for maximum yield. Including lysis, isolated DNA is available in approximately 24 min. The isolated DNA is suitable for a wide range of different molecular biology applications.
Protocols are available for isolating DNA from 200 µl or 400 µl whole blood samples.
The GenUP Blood DNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP gDNA Kit (cat. no. BR0700601), GenUP Bacteria gDNA Kit (cat. no. BR0700701), GenUP Plant DNA Kit (cat. no. BR0700801) or GenUP Virus DNA/RNA Kit (cat. no. BR0701101).
biotechrabbit GenUP™ gDNA Kit is designed for a fast and efficient genomic DNA isolation from various sources and different amounts of starting material, such as mammalian tissues (including paraffin-embedded), buccal swabs and eukaryotic cell cultures. After a few initial procedures, the genomic DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. High yields of pure, unshared genomic DNA are ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
biotechrabbit™ GenUP Bacterial gDNA Kit has been specially developed for quick and easy purification of bacterial genomic DNA from both Gram-negative and difficult to process Gram-positive bacteria.
A combined lysozyme and proteolytic lysis steps allow efficient cell disruption. The DNA is bound to a high-capacity filter, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including PCR, enzymatic digestions, cloning and other.
biotechrabbit™ GenUP Plant DNA Kit has been specially developed for quick and easy purification of genomic DNA from a wide variety of plant materials, including fresh, frozen or dried samples from leaves, roots, stems and flowers.
The kit includes an advanced prefiltration step to remove unlysed tissue. Subsequently, DNA is bound to a Mini Filter and is subsequently washed and eluted in a separate tube. The purified DNA is ready for use in any demanding molecular biology application, including PCR, enzymatic digestions and cloning.
This kit provides three buffers for optimized processing with different plant materials. To determine optimal lysis conditions, side-by-side preparation using the three provided protocols are prepared.
biotechrabbit™ GenUP Blood RNA Kit is designed for fast isolation of total RNA from up to 1 ml whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. Erythrocytes are removed in an initial lysis step. After an second lysis step, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. Including lysis, isolated RNA is available in approximately 45 min. The isolated RNA is suitable for a wide range of different molecular biology applications, including RT-PCR.
The GenUP Blood RNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP Total RNA Kit (BR07009), GenUP Virus RNA Kit (BR07010), GenUP Virus DNA/RNA Kit (BR07011) or UPzol RNA Isolation Solution (BR07001).
biotechrabbit™ GenUP Total RNA Kit has been specially developed for a quick and easy purification of total RNA from eukaryotic cell suspensions, tissues and biopsies, Gram-negative (e.g., E. coli) and Gram-positive bacteria and other sources. After few initial procedures, the RNA is bound to a filter, washed and then eluted in a separate tube. DNA is removed physically by binding to a filter without any DNAse treatment or the use of toxic β‑mercaptoethanol. The purified RNA is ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis and others.
biotechrabbit™ GenUP Virus DNA/RNA Kit has been specially developed for quick and easy isolation of viral RNA and DNA. The kit is especially useful when the origin of the virus is unknown. Viral double-stranded DNA and single-stranded RNA are simultaneously isolated from eukaryotic samples, including plasma, serum, and other body fluids as well as cell cultures, tissues, and buccal swabs.
The unique binding membrane of our high-capacity Mini Filters guaranties high yields. A high concentration of purified nucleic acid can be achieved with flexible elution volumes. The kit includes carrier RNA.
After a few initial procedures, the viral nucleic acids are bound to a Mini Filter, washed and then eluted in a separate tube. The purified nucleic acids are ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis, qPCR and RT-PCR.
biotechrabbit™ GenUP FFPE Paraffin Removal Solution is an odor-free, biofriendly and innovative solution to deparaffinize FFPE slices within minutes in a single step. This product replaces conventional procedures that involve toxic and/or flammable solvents. After removal of paraffin from FFPE slices with GenUP FFPE Paraffin Removal Solution, continue with the genomic DNA or RNA purification kit of your choice.
biotechrabbit™ GenUP Plant RNA Kit has been developed for quick and easy purification of total RNA from plant materials. After initial homogenization and lysis, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. The purified RNA is ready for use in any demanding molecular biology application, including RT-PCR.
Two lysis buffers, Buffer LYSIS LR and Buffer LYSIS LT, are provided to maximize yield. Most plant material can be processed with Buffer LYSIS LR. In the cases that yield using Buffer LYSIS LR is low, use Buffer LYSIS LT.
biotechrabbit™ GenUP Micro RNA Kit is designed for high yields of small RNA molecules and total RNA, including snRNA, miRNA, siRNA, tRNA, rRNA and mRNA, without the use of highly toxic β-mercaptoethanol. After using well established filter-technology to selectively remove genomic DNA, the RNA is bound, washed and eluted from the filter membrane using RNase-free water.
biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.
The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Heat Labile Exonuclease 1 (HL-ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.
Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 3' - 5' exonuclease activity of Exonuclease 1 degrades single-stranded primers.
biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.
The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
biotechrabbit™ GenUP PCR Cleanup Kit has been developed for quick and easy cleanup or concentration of PCR fragments from reaction mixtures. High-yield PCR product with excellent quality is purified from the PCR mixtures in a simple two-step procedure. DNA is bound to a Mini Filter using a novel buffer and eluted. The need for a washing step has been eliminated, reducing hands-on-time. The procedure takes approximately 3 min, compared to 8 min required to other kits. The purified DNA is ready for use in all demanding molecular biology applications, including restriction digestion, ligation and sequencing.