即用型FPLC预装柱，预装Protino® Glutathione Agarose 4B，用于亲和纯化GST-tag蛋白 Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B，the cost-effective alternative for GST-tag protein purification • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns • Simply replace your current products, no optimization or protocol change necessary • Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use • Market-leading performance for an unbeatable price
FPLC column (5 mL) for purification of GST-tag proteins pack of 1
更具性价比的GST-tag蛋白亲和纯化填料，适用范围广 • Aqueous suspension, the cost-effective alternative for GST-tag protein purification • For batch binding, gravity flow, and FPLC™ → highest flexibility of applications • Empty Protino® columns for gravity flow available • Simply replace your current products, no optimization or protocol change necessary • Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use • Market-leading performance for an unbeatable price
Technology Affinity chromatography Chelating ligand Glutathione, linked via sulfur atom Format 75 % (v/v) aqueous suspension containing 20 % ethanol Matrix: 4 % beaded agarose Bead size: 90 µm Binding capacity* :> 8 mg/mL Recommended flow rates: 1 mL bed volume (column with 6.6 mm inner diameter): Sample loading**: 0.2–1.0 mL/min. Washing / elution: 1.0 mL/min 10 mL bed volume (column with 16 mm inner diameter): Sample loading**: 0.5–5.0 mL/min. Washing / elution: 5.0 mL/min Max. linear flow rate 250 cm/h Chemical stability: Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4, 0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield Storage temperature 4–8 °C Note Do not autoclave the gel * Binding capacity will vary for each GST-tagged protein ** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein
即用型FPLC预装柱，预装Protino® Glutathione Agarose 4B，用于亲和纯化GST-tag蛋白 Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B，the cost-effective alternative for GST-tag protein purification • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns • Simply replace your current products, no optimization or protocol change necessary • Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use • Market-leading performance for an unbeatable price
FPLC columns (1 mL) for purification of GST-tag proteins pack of 5
即用型FPLC预装柱，预装Protino® Glutathione Agarose 4B，用于亲和纯化GST-tag蛋白 Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B，the cost-effective alternative for GST-tag protein purification • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns • Simply replace your current products, no optimization or protocol change necessary • Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use • Market-leading performance for an unbeatable price
FPLC columns (5 mL) for purification of GST-tag proteins pack of 5
更具性价比的GST-tag蛋白亲和纯化填料，适用范围广 • Aqueous suspension, the cost-effective alternative for GST-tag protein purification • For batch binding, gravity flow, and FPLC™ → highest flexibility of applications • Empty Protino® columns for gravity flow available • Simply replace your current products, no optimization or protocol change necessary • Suitable for small proteins, large protein complexes, proteins with low expression rates → universal use • Market-leading performance for an unbeatable price
Technology Affinity chromatography Chelating ligand Glutathione, linked via sulfur atom Format 75 % (v/v) aqueous suspension containing 20 % ethanol Matrix: 4 % beaded agarose Bead size: 90 µm Binding capacity* :> 8 mg/mL Recommended flow rates: 1 mL bed volume (column with 6.6 mm inner diameter): Sample loading**: 0.2–1.0 mL/min. Washing / elution: 1.0 mL/min 10 mL bed volume (column with 16 mm inner diameter): Sample loading**: 0.5–5.0 mL/min. Washing / elution: 5.0 mL/min Max. linear flow rate 250 cm/h Chemical stability: Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4, 0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield Storage temperature 4–8 °C Note Do not autoclave the gel * Binding capacity will vary for each GST-tagged protein ** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein
