Shelf Life(month)：24
产地：USA (美国)
存储条件：-20
此起始(顶端)caspase蛋白小包装抗体套装包含Caspase-2 (C2) Mouse mAb 2224T、Anti-rabbit IgG, HRP-linked Antibody 7074P2、Anti-mouse IgG, HRP-linked Antibody 7076P2、Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 7237T、 Caspase-9 (C9) Mouse mAb 9508T、 Caspase-3 (8G10) Rabbit mAb 9665T 和 Caspase-8 (1C12) Mouse mAb 9746T。Initiator Caspases Antibody Sampler试剂盒提供了一种经济的方法，检测起始(顶端)caspase蛋白。该试剂盒含有的一抗，足够进行四次western blot实验。Initiator Caspases Antibody Sampler试剂盒中的每个抗体都能识别对应的内源性靶蛋白。Caspase-3 (8G10) Rabbit mAb 兔单抗能够检测caspase-3全长(35 kDa)和在Asp175位点裂解产生的caspase-3大片段(17/19 kDa)。Caspase-8 (1C12) Mouse mAb 鼠单抗能够检测caspase-8的全长(57 kDa)，及裂解产生的中间体p43/p41以及p18片段。Caspase-9 (C9) Antibody能够检测全长caspase-9，以及Asp315和 Asp330裂解产生的片段。Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 兔单抗仅能够检测在Asp330处裂解产生的caspase-9片段。Caspase-2 (C2) Mouse mAb 鼠单抗能够识别procaspase-2，以及其14和12 kDa的裂解片段。单克隆抗体由重组人caspase-9蛋白或合成的肽段免疫动物产生，这些肽段与人caspase-3的氨基端残基Asp175附近序列，人capase-9的Asp330附近序列，人caspase-8的p18片段羧基端序列，或人caspase-2的羧基端序列一致。The Initiator Caspases Antibody Sampler Kit provides an economical means of evaluating initiator (apical) caspase proteins. The kit contains enough primary antibody to perform four western blots with each primary antibody.Each antibody in the Initiator Caspases Antibody Sampler Kit recognizes endogenous levels of its respective target. Caspase-3 (8G10) Rabbit mAb recognizes full-length (35 kDa) and the large fragment (17/19 kDa) of caspase-3 resulting from cleavage at aspartic acid 175. Caspase-8 (1C12) Mouse mAb recognizes full length (57 kDa), the cleaved intermediate p43/p41, and the p18 fragment of caspase-8. Caspase-9 (C9) Antibody recognizes full-length caspase-9, as well as the large fragments resulting from cleavage at Asp315 and Asp330. Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb recognizes caspase-9 only when cleaved at Asp330. Caspase-2 (C2) Mouse mAb recognizes procaspase-2, as well as its 14 and 12 kDa cleaved fragments.Monoclonal antibodies are produced by immunizing animals with a recombinant human caspase-9 protein or with a synthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3, residues surrounding Asp330 of human caspase-9, the carboxy-terminal sequence of the p18 fragment of human caspase-8, or the carboxy-terminal portion of human caspase-2.