产地：USA (美国)
存储条件：常温
该产品是 RNA 纯化浓缩试剂盒，采用了简单快速的硅胶膜离心技术，适用于酶学反应后 RNA，如：体外转录（IVT）、 DNaseI 处理、加帽和标记等；也适用于 RNA提取后的样本，如：苯酚/氯仿提取的 RNA。NEB 提供三种不同结合力的试剂盒：10 μg、50 μg 和500μg，每一款试剂盒所用纯化柱都是经过独特设计以确保无缓冲液、无残留污染、低洗脱体积，可获得高纯度RNA。按着标准说明书，适合纯化RNA ≥25 nt的片段。通过调整说明书，可将回收片段大小从 25 nt降低到 15 nt（包括miRNAs）。 The Monarch RNA Cleanup Kit (50 µg) rapidly and reliably purifies up to 50 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).
试剂盒成分：Monarch® Collection Tubes II、Monarch® RNA Cleanup Binding Buffer、Monarch® RNA Cleanup Wash Buffer、Monarch® RNA Cleanup Columns (50 μg) 储存温度：25°C